公开(公告)号：US20210163926A1

公开(公告)日：2021-06-03

申请号：US16954201

申请日：2019-01-03

Applicant: ARIZONA BOARD OF REGENTS ON BEHALF OF ARIZONA STATE UNIVERSITY

Inventor： Joshua LABAER ,  Jin PARK

IPC: C12N15/10

Abstract: Disclosed is a method of functional genomics determination including transducing a cell population with a set of nucleic acid molecules including a pooled library of genomic perturbagens to integrate multiple perturbagen cassettes into the genome. A phenotype of individual cells is determined and single cells of the population with targeted phenotypes are individually sorted into a set of compartments. Each compartment includes a forward primer with a nucleic acid sequence (NAS) that specifically binds a common nucleic acid sequence on the nucleic acid molecules and a compartment (cell)-specific nucleic acid barcode. Also included is a reverse primer with a NAS that specifically binds a common NAS on the nucleic acid molecules comprising a pooled library of genomic perturbagens. The genome-integrated perturbagen cassettes are create amplicons which are pooled and sequences determined. This method can be applied to other genome-level single-cell applications—immune receptor profiling, targeted DNA/RNA sequencing, and metagenomics.

公开(公告)号：US20230383339A1

公开(公告)日：2023-11-30

申请号：US18248215

申请日：2021-10-07

Applicant: ARIZONA BOARD OF REGENTS ON BEHALF OF ARIZONA STATE UNIVERSITY

Inventor： Joshua LABAER ,  Vel MURUGAN ,  Inam RIDHA

IPC: C12Q1/6855 ,  C12N15/10 ,  C12Q1/6876

CPC classification number: C12Q1/6855 ,  C12N15/1093 ,  C12Q1/6876 ,  C12Q2600/154

Abstract: The disclosure relates to methods for multiplexed detection of DNA methylation in pooled DNA libraries. In particular, provided herein are methods for detection of DNA methylation in pooled DNA libraries using enzymatic digestion by a micrococcal nuclease, methylated DNA immunoprecipitation, and quantitative amplification.

公开(公告)号：US20230375538A1

公开(公告)日：2023-11-23

申请号：US18017563

申请日：2021-07-22

Applicant: ARIZONA BOARD OF REGENTS ON BEHALF OF ARIZONA STATE UNIVERSITY

Inventor： Joshua LABAER ,  Jin PARK ,  Femina RAUF

IPC: G01N33/537 ,  G01N33/58

CPC classification number: G01N33/537 ,  G01N33/58 ,  G01N2458/10

Abstract: Provided herein are compositions comprising coordinated sets of unique DNA barcodes and methods for using the same for multiplex detection and measurement of multiple target molecules in multiple samples using a single next-generation sequencing reaction. In particular, methods are provided in which unique DNA barcodes linked to affinity reagents are contacted to a sample to bind antigens if present in said sample, and then a PCR-based amplification reaction adds barcoded index sequences that contain universal sequencing adaptors as well as unique barcode sequences and amplifies affinity reagent-bound targets for DNA sequencing.

公开(公告)号：US20180050038A1

公开(公告)日：2018-02-22

申请号：US15555241

申请日：2016-03-02

Applicant: Arizona Board of Regents on behalf of Arizona State University

Inventor： Fernanda FESTA ,  Joshua LABAER ,  Jin PARK ,  Femina RAUF

IPC: A61K31/519 ,  A61K31/517

CPC classification number: A61K31/519 ,  A61K31/517

Abstract: Disclosed herein are methods of treating diseases that exhibit over-activation of the ERBB4 pathway, such as breast cancer and lung cancer. The method comprises administering to a subject having a disease that exhibits over-activation of the ERBB4 pathway and a gene expression profile consistent with an ERBB4-sensitive profile a therapeutically effective amount of an ERBB4 inhibitor, wherein the disease is effectively treated. Gene expression profiles of ERBB4-sensitive cells and microarrays suitable for protein-tyrosine kinases are also provided.

公开(公告)号：US20240053336A1

公开(公告)日：2024-02-15

申请号：US18448685

申请日：2023-08-11

Applicant: Arizona Board of Regents on Behalf of Arizona State University ,  Mayo Foundation for Medical Education & Research

Inventor： Mahasish SHOME ,  Lusheng SONG ,  Yunro CHUNG ,  Jonathan LEIGHTON ,  Joshua LABAER ,  Ji QIU

IPC: G01N33/569 ,  G01N33/68

CPC classification number: G01N33/56911 ,  G01N33/686 ,  G01N2800/065

Abstract: The present invention relates anti-microbial antibody signatures of inflammatory bowel disease and their use in early and accurate diagnosis of disease including ulcerative colitis (UC) and Crohn's disease (CD).

公开(公告)号：US20230384306A1

公开(公告)日：2023-11-30

申请号：US18326837

申请日：2023-05-31

Applicant: Arizona Board of Regents on Behalf of Arizona State University

Inventor： Mahasish SHOME ,  Yunro CHUNG ,  Ramani CHAVAN ,  Jin PARK ,  Ji QIU ,  Joshua LABAER

IPC: G01N33/564 ,  G01N33/569 ,  G01N33/574

CPC classification number: G01N33/564 ,  G01N33/56983 ,  G01N33/57484

Abstract: The present invention relates autoantibody biomarkers, kits, and methods for increasing the accuracy of a cancer or autoimmune screening of a subject suspected of having cancer or an autoimmune condition.