公开(公告)号：US10557807B2

公开(公告)日：2020-02-11

申请号：US16328262

申请日：2018-05-22

Applicant: ARIZONA BOARD OF REGENTS ON BEHALF OF ARIZONA STATE UNIVERSITY

Inventor： Alexandra Ros ,  Gerrit Brehm ,  Garrett Nelson ,  John Spence ,  Uwe Weierstall ,  Austin Echelmeier

IPC: G01N23/2005 ,  B01F13/00 ,  B33Y80/00 ,  G01N23/20025 ,  B01L3/00

Abstract: A 3D printed hybrid nozzle device combining a microfluidic mixer with a liquid jet injector that addresses the bottleneck of investigating substrate-initiated biological reaction paths employing serial crystallography with XFELs. The hybrid nozzle provides for injecting aqueous protein crystal jets after fast mixing (

公开(公告)号：US11485632B2

公开(公告)日：2022-11-01

申请号：US17497675

申请日：2021-10-08

Applicant: ARIZONA BOARD OF REGENTS ON BEHALF OF ARIZONA STATE UNIVERSITY

Inventor： Alexandra Ros ,  John Spence ,  Diandra Doppler ,  Garrett Nelson ,  Richard Kirian ,  Reza Nazari ,  Ana Egatz-Gomez ,  Mukul Sonker ,  Mohammad Rabbani

IPC: B33Y80/00 ,  B81C3/00 ,  B81B1/00

Abstract: A microfluidic device for use in a serial crystallography apparatus includes a modular 3D-printed nozzle having an inlet, an outlet, and a first snap engagement feature. The microfluidic device further includes a modular 3D-printed fiber holder having an outlet and a second snap engagement feature. The first snap engagement feature is configured to engage the second snap engagement feature to removably couple the nozzle to the fiber holder. The outlet of the fiber holder is aligned with the inlet of the nozzle when the first snap engagement feature is coupled to the second snap engagement feature.

公开(公告)号：US09821325B2

公开(公告)日：2017-11-21

申请号：US14780766

申请日：2014-04-28

Applicant: ARIZONA BOARD OF REGENTS on behalf of ARIZONA STATE UNIVERSITY

Inventor： Uwe Weierstall ,  John Spence ,  R. Bruce Doak

IPC: B05B1/02 ,  B05B1/10 ,  B05B7/06 ,  B05B7/08 ,  C30B7/00 ,  C30B29/58 ,  B05B7/24 ,  C30B7/02

CPC classification number: B05B1/10 ,  B05B1/02 ,  B05B7/064 ,  B05B7/066 ,  B05B7/08 ,  B05B7/24 ,  C30B7/00 ,  C30B7/02 ,  C30B29/58

Abstract: An injector apparatus and methods for use, where the injector apparatus comprises: (a) hydraulic stage having first and second ends and including a housing defining a cavity, a primary plunger disposed in the cavity and a secondary plunger, (b) a pressurization system coupled to the hydraulic stage's first end, where the primary plunger is in fluid communication with the pressurization system and is in mechanical communication with the secondary plunger, (c) a reservoir bore defined in the hydraulic stage housing and configured to receive the primary plunger's second end, where the secondary plunger is disposed within the reservoir bore and (d) a nozzle assembly including a housing, a gas tube and a nozzle capillary, where the nozzle capillary is partially disposed in and is substantially coaxial with the gas tube, where the nozzle capillary's first end is in fluid communication with the reservoir bore's second end.