公开(公告)号：US12018317B2

公开(公告)日：2024-06-25

申请号：US17546953

申请日：2021-12-09

Applicant: ARIZONA BOARD OF REGENTS ON BEHALF OF ARIZONA STATE UNIVERSITY

Inventor： Joseph Blattman ,  Louis Schoettle

IPC: C12Q1/68 ,  C12N15/87 ,  C12Q1/6806 ,  C12Q1/6869

CPC classification number: C12Q1/6806 ,  C12N15/87 ,  C12Q1/6869 ,  C12Q1/6869 ,  C12Q2563/159 ,  C12Q2563/179 ,  C12Q2565/133

Abstract: Methods for incorporation of unique bowtie-barcodes into a nucleic acid origami nanostructure (FIG. 1). In particular, provided herein are methods that facilitate pairing and analysis of nucleic acids from individual cells using, for example, high-throughput next-generation sequencing.

公开(公告)号：US20180216102A1

公开(公告)日：2018-08-02

申请号：US15937371

申请日：2018-03-27

Applicant: ARIZONA BOARD OF REGENTS ON BEHALF OF ARIZONA STATE UNIVERSITY

Inventor： Joseph Blattman ,  Hao Yan ,  Louis Schoettle ,  Xixi Wei

IPC: C12N15/10 ,  C12Q1/6806

CPC classification number: C12N15/1062 ,  B82Y5/00 ,  B82Y15/00 ,  C12Q1/6806 ,  C12Q1/6816 ,  C12Q1/6881 ,  C12Q2600/16 ,  C12Q2563/155

Abstract: Methods for construction of DNA origami nanostructures, as well as for binding, isolation, linking, and deep sequencing information, such as both of TCR alpha and beta CDR3 mRNA, from individual cells within a mixed population of cells without the need for single cell sorting (FIG. 1).

公开(公告)号：US20220162674A1

公开(公告)日：2022-05-26

申请号：US17546953

申请日：2021-12-09

Applicant: ARIZONA BOARD OF REGENTS ON BEHALF OF ARIZONA STATE UNIVERSITY

Inventor： Joseph Blattman ,  Louis Schoettle

IPC: C12Q1/6806 ,  C12Q1/6869 ,  C12N15/87

Abstract: Methods for incorporation of unique bowtie-barcodes into a nucleic acid origami nanostructure (FIG. 1). In particular, provided herein are methods that facilitate pairing and analysis of nucleic acids from individual cells using, for example, high-throughput next-generation sequencing.

公开(公告)号：US11466267B2

公开(公告)日：2022-10-11

申请号：US15937371

申请日：2018-03-27

Applicant: ARIZONA BOARD OF REGENTS ON BEHALF OF ARIZONA STATE UNIVERSITY

Inventor： Joseph Blattman ,  Hao Yan ,  Louis Schoettle ,  Xixi Wei

IPC: C12Q1/68 ,  C12N15/10 ,  C12Q1/6816 ,  C12Q1/6881 ,  C12Q1/6806 ,  B82Y5/00 ,  B82Y15/00

Abstract: Methods for construction of DNA origami nanostructures, as well as for binding, isolation, linking, and deep sequencing information, such as both of TCR alpha and beta CDR3 mRNA, from individual cells within a mixed population of cells without the need for single cell sorting (FIG. 1).

公开(公告)号：US11208681B2

公开(公告)日：2021-12-28

申请号：US16320962

申请日：2017-08-14

Applicant: ARIZONA BOARD OF REGENTS ON BEHALF OF ARIZONA STATE UNIVERSITY

Inventor： Joseph Blattman ,  Louis Schoettle

IPC: C12Q1/68 ,  C12Q1/6806 ,  C12Q1/6869 ,  C12N15/87

Abstract: Methods for incorporation of unique bowtie-barcodes into a nucleic acid origami nanostructure (FIG. 1). In particular, provided herein are methods that facilitate pairing and analysis of nucleic acids from individual cells using, for example, high-throughput next-generation sequencing.

公开(公告)号：US20190194724A1

公开(公告)日：2019-06-27

申请号：US16320962

申请日：2017-08-14

Applicant: ARIZONA BOARD OF REGENTS ON BEHALF OF ARIZONA STATE UNIVERSITY

Inventor： Joseph Blattman ,  Louis Schoettle

IPC: C12Q1/6806 ,  C12Q1/6869

CPC classification number: C12Q1/6806 ,  C12N15/87 ,  C12Q1/6869 ,  C12Q2563/159 ,  C12Q2563/179 ,  C12Q2565/133

Abstract: Methods for incorporation of unique bowtie-barcodes into a nucleic acid origami nanostructure (FIG. 1). In particular, provided herein are methods that facilitate pairing and analysis of nucleic acids from individual cells using, for example, high-throughput next-generation sequencing.