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1.发明申请公开(公告)号：US20170145465A1
公开(公告)日：2017-05-25
申请号：US15425003
申请日：2017-02-06
Applicant: AbbVie Inc.
Inventor： Alane E. Wentz , Don Hemmavanh , Joseph G. Matuck
CPC classification number: C12P21/005 , C07K16/00 , C07K16/2863 , C07K2317/14 , C07K2317/24 , C07K2317/31 , C07K2317/41 , C07K2317/64 , C12N5/0018 , C12N5/005 , C12N2500/24 , C12N2510/02
Abstract: Protein glycosylation greatly influences the structure, function, and pharmacokinetics of recombinant proteins. Here, growth media supplemented with metal ions is shown to modulate the protein glycosylation profile of recombinant proteins expressed in a variety of eukaryotic cell lines. In particular, millimolar amounts of ferric salts (e.g. ferric nitrate, ferric citrate, etc.) significantly increased the percentage of galactosylated N-glycan species. The effect on protein glycosylation was concentration-dependent manner up to 1.0 mM across multiple cell lines without adverse effects on cell viability. The use of iron supplements in cell culture media provides an efficient and effective approach towards the specific re-targeting of N-glycan glycoform profiles of recombinantly expressed proteins.
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2.发明授权公开(公告)号：US09598667B2
公开(公告)日：2017-03-21
申请号：US14506372
申请日：2014-10-03
Applicant: AbbVie, Inc.
Inventor： Alane E. Wentz , Don Hemmavanh , Joseph G. Matuck
CPC classification number: C12P21/005 , C07K16/00 , C07K16/2863 , C07K2317/14 , C07K2317/24 , C07K2317/31 , C07K2317/41 , C07K2317/64 , C12N5/0018 , C12N5/005 , C12N2500/24 , C12N2510/02
Abstract: Protein glycosylation greatly influences the structure, function, and pharmacokinetics of recombinant proteins. Here, growth media supplemented with metal ions is shown to modulate the protein glycosylation profile of recombinant proteins expressed in a variety of eukaryotic cell lines. In particular, millimolar amounts of ferric salts (e.g. ferric nitrate, ferric citrate, etc.) significantly increased the percentage of galactosylated N-glycan species. The effect on protein glycosylation was concentration-dependent manner up to 1.0 mM across multiple cell lines without adverse effects on cell viability. The use of iron supplements in cell culture media provides an efficient and effective approach towards the specific re-targeting of N-glycan glycoform profiles of recombinantly expressed proteins.
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公开(公告)号：US20140206038A1
公开(公告)日：2014-07-24
申请号：US14226333
申请日：2014-03-26
Applicant: AbbVie Inc.
Inventor： Itzcoatl A. Pla , Joseph G. Matuck , John C. Fann , Christof Schulz , Nichole A. Roy , David F. Bruton , James McIntire , Yu-Hsiang David Chang , Thomas Seewoester
IPC: C07K16/24
CPC classification number: C12N5/0037 , C07K16/00 , C07K16/241 , C07K16/244 , C07K16/2869 , C07K2317/14 , C07K2317/21 , C12N5/0031 , C12N5/0603 , C12N2500/24 , C12N2500/25 , C12N2500/30 , C12N2500/32 , C12N2500/34 , C12N2500/38 , C12N2500/50 , C12N2500/60 , C12N2500/74 , C12N2500/76 , C12N2500/90 , C12N2501/10 , C12N2501/33 , C12N2510/02 , C12N2511/00
Abstract: The invention describes improved methods and compositions for producing a recombinant protein, e.g., an antibody, in mammalian cell culture. In addition, the invention provides improved cell culture media, including improved production media, feed solutions, and combination feeds, which may be used to improve protein productivity in mammalian cell culture.
Abstract translation: 本发明描述了用于在哺乳动物细胞培养物中产生重组蛋白例如抗体的改进的方法和组合物。 此外，本发明提供改进的细胞培养基，包括改良的生产培养基，饲料溶液和组合饲料，其可用于改善哺乳动物细胞培养物中的蛋白质生产力。
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公开(公告)号：US20170130191A1
公开(公告)日：2017-05-11
申请号：US15187425
申请日：2016-06-20
Applicant: AbbVie Inc.
Inventor： Itzcoatl A. Pla , Joseph G. Matuck , John C. Fann , Christof Schulz , Nichole A. Roy , David F. Bruton , James McIntire , Yu-hsiang D. Chang , Thomas Seewoester
IPC: C12N5/00
CPC classification number: C12N5/0037 , C07K16/00 , C07K16/241 , C07K16/244 , C07K16/2869 , C07K2317/14 , C07K2317/21 , C12N5/0031 , C12N5/0603 , C12N2500/24 , C12N2500/25 , C12N2500/30 , C12N2500/32 , C12N2500/34 , C12N2500/38 , C12N2500/50 , C12N2500/60 , C12N2500/74 , C12N2500/76 , C12N2500/90 , C12N2501/10 , C12N2501/33 , C12N2510/02 , C12N2511/00
Abstract: The invention describes improved methods and compositions for producing a recombinant protein, e.g., an antibody, in mammalian cell culture. In addition, the invention provides improved cell culture media, including improved production media, feed solutions, and combination feeds, which may be used to improve protein productivity in mammalian cell culture.
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公开(公告)号：US09234032B2
公开(公告)日：2016-01-12
申请号：US14157460
申请日：2014-01-16
Applicant: AbbVie Inc.
Inventor： Itzcoatl A. Pla , Joseph G. Matuck , John C. Fann , Christof Schulz , Nichole A. Roy , David F. Bruton , James McIntire , Yu-hsiang D. Chang , Thomas Seewoester
CPC classification number: C12N5/0037 , C07K16/00 , C07K16/241 , C07K16/244 , C07K16/2869 , C07K2317/14 , C07K2317/21 , C12N5/0031 , C12N5/0603 , C12N2500/24 , C12N2500/25 , C12N2500/30 , C12N2500/32 , C12N2500/34 , C12N2500/38 , C12N2500/50 , C12N2500/60 , C12N2500/74 , C12N2500/76 , C12N2500/90 , C12N2501/10 , C12N2501/33 , C12N2510/02 , C12N2511/00
Abstract: The invention describes improved methods and compositions for producing a recombinant protein, e.g., an antibody, in mammalian cell culture. In addition, the invention provides improved cell culture media, including improved production media, feed solutions, and combination feeds, which may be used to improve protein productivity in mammalian cell culture.
Abstract translation: 本发明描述了用于在哺乳动物细胞培养物中产生重组蛋白例如抗体的改进的方法和组合物。 此外，本发明提供改进的细胞培养基，包括改良的生产培养基，饲料溶液和组合饲料，其可用于改善哺乳动物细胞培养物中的蛋白质生产力。
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Patent Agency Ranking
6.发明申请Use of Metal Ions for Modulation of Protein Glycosylation Profiles of Recombinant Proteins 有权使用金属离子来调节重组蛋白质的蛋白质糖基化谱公开(公告)号：US20150133639A1
公开(公告)日：2015-05-14
申请号：US14506372
申请日：2014-10-03
Applicant: AbbVie, Inc.
Inventor： Alane E. Wentz , Don Hemmavanh , Joseph G. Matuck
CPC classification number: C12P21/005 , C07K16/00 , C07K16/2863 , C07K2317/14 , C07K2317/24 , C07K2317/31 , C07K2317/41 , C07K2317/64 , C12N5/0018 , C12N5/005 , C12N2500/24 , C12N2510/02
Abstract: Protein glycosylation greatly influences the structure, function, and pharmacokinetics of recombinant proteins. Here, growth media supplemented with metal ions is shown to modulate the protein glycosylation profile of recombinant proteins expressed in a variety of eukaryotic cell lines. In particular, millimolar amounts of ferric salts (e.g. ferric nitrate, ferric citrate, etc.) significantly increased the percentage of galactosylated N-glycan species. The effect on protein glycosylation was concentration-dependent manner up to 1.0 mM across multiple cell lines without adverse effects on cell viability. The use of iron supplements in cell culture media provides an efficient and effective approach towards the specific re-targeting of N-glycan glycoform profiles of recombinantly expressed proteins.
Abstract translation: 蛋白质糖基化大大影响重组蛋白的结构，功能和药代动力学。 在这里，补充有金属离子的生长培养基显示调节在各种真核细胞系中表达的重组蛋白质的蛋白质糖基化谱。 特别地，毫摩尔量的铁盐（例如硝酸铁，柠檬酸铁等）显着增加半乳糖基化的N-聚糖物质的百分比。 对蛋白质糖基化的影响是浓度依赖性方式，多达1.0mM，不影响细胞活力。 在细胞培养基中使用铁补充剂为重组表达蛋白质的N-聚糖糖基型的特异性再靶向提供了有效和有效的方法。
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公开(公告)号：US20190225934A1
公开(公告)日：2019-07-25
申请号：US16118405
申请日：2018-08-30
Applicant: AbbVie Inc.
Inventor： Itzcoatl A. Pla , Joseph G. Matuck , John C. Fann , Christof Schulz , Nichole A. Roy , David F. Bruton , James McIntire , Yu-hsiang D. Chang , Thomas Seewoester
Abstract: The invention describes improved methods and compositions for producing a recombinant protein, e.g., an antibody, in mammalian cell culture. In addition, the invention provides improved cell culture media, including improved production media, feed solutions, and combination feeds, which may be used to improve protein productivity in mammalian cell culture.
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公开(公告)号：US20170355760A1
公开(公告)日：2017-12-14
申请号：US15588211
申请日：2017-05-05
Applicant: AbbVie Inc.
Inventor： Patrick Hossler , Sean McDermott , Christopher Racicot , Joseph G. Matuck , Keith Cochran , Christopher M. Chumsae
CPC classification number: C07K16/241 , C07K16/00 , C07K2317/14 , C07K2317/24 , C07K2317/41 , C12N5/0037 , C12N2500/34 , C12N2510/02 , C12P21/005
Abstract: The present invention relates to the field of protein production, and in particular to methods and compositions for modulating glycosylation of recombinant proteins expressed in host cells.
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公开(公告)号：US20160186130A1
公开(公告)日：2016-06-30
申请号：US15069456
申请日：2016-03-14
Applicant: AbbVie Inc.
Inventor： Itzcoatl A. Pla , Joseph G. Matuck , John C. Fann , Christof Schulz , Nichole A. Roy , David F. Bruton , James McIntire , Yu-Hsiang D. Chang , Thomas Seewoester
IPC: C12N5/00
CPC classification number: C12N5/0037 , C07K16/00 , C07K16/241 , C07K16/244 , C07K16/2869 , C07K2317/14 , C07K2317/21 , C12N5/0031 , C12N5/0603 , C12N2500/24 , C12N2500/25 , C12N2500/30 , C12N2500/32 , C12N2500/34 , C12N2500/38 , C12N2500/50 , C12N2500/60 , C12N2500/74 , C12N2500/76 , C12N2500/90 , C12N2501/10 , C12N2501/33 , C12N2510/02 , C12N2511/00
Abstract: The invention describes improved methods and compositions for producing a recombinant protein, e.g., an antibody, in mammalian cell culture. In addition, the invention provides improved cell culture media, including improved production media, feed solutions, and combination feeds, which may be used to improve protein productivity in mammalian cell culture.
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公开(公告)号：US09284371B2
公开(公告)日：2016-03-15
申请号：US14920452
申请日：2015-10-22
Applicant: AbbVie Inc.
Inventor： Itzcoatl A. Pla , Joseph G. Matuck , John C. Fann , Christof Schulz , Nichole A. Roy , David F. Bruton , James McIntire , Yu-Hsiang D. Chang , Thomas Seewoester
IPC: C07K16/24
CPC classification number: C12N5/0037 , C07K16/00 , C07K16/241 , C07K16/244 , C07K16/2869 , C07K2317/14 , C07K2317/21 , C12N5/0031 , C12N5/0603 , C12N2500/24 , C12N2500/25 , C12N2500/30 , C12N2500/32 , C12N2500/34 , C12N2500/38 , C12N2500/50 , C12N2500/60 , C12N2500/74 , C12N2500/76 , C12N2500/90 , C12N2501/10 , C12N2501/33 , C12N2510/02 , C12N2511/00
Abstract: The invention describes improved methods and compositions for producing a recombinant protein, e.g., an antibody, in mammalian cell culture. In addition, the invention provides improved cell culture media, including improved production media, feed solutions, and combination feeds, which may be used to improve protein productivity in mammalian cell culture.
Abstract translation: 本发明描述了用于在哺乳动物细胞培养物中产生重组蛋白例如抗体的改进的方法和组合物。 此外，本发明提供改进的细胞培养基，包括改良的生产培养基，饲料溶液和组合饲料，其可用于改善哺乳动物细胞培养物中的蛋白质生产力。
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