公开(公告)号：US20170002075A1

公开(公告)日：2017-01-05

申请号：US15213130

申请日：2016-07-18

Applicant: ABBVIE DEUTSCHLAND GMBH & CO. KG ,  ABBVIE INC.

Inventor： Jijie GU ,  Chung-Ming HSIEH ,  Zhen WU ,  Enrico L. DiGIAMMARINO ,  Feng LUO ,  Gerard B. FOX ,  John E. HARLAN ,  Martin SCHMIDT ,  Ralf LOEBBERT ,  Reinhold MUELLER ,  Ulrich EBERT ,  Volker NIMMRICH

IPC: C07K16/28 ,  C07K16/46 ,  C12P21/00

CPC classification number: C07K16/2803 ,  A61K2039/505 ,  C07K16/28 ,  C07K16/464 ,  C07K2317/14 ,  C07K2317/24 ,  C07K2317/56 ,  C07K2317/565 ,  C07K2317/567 ,  C07K2317/76 ,  C07K2317/92 ,  C12P21/005 ,  Y02A50/466

Abstract: The present application relates to isolated proteins, particularly monoclonal antibodies, in particular CDR-grafted, humanized antibodies which bind to RAGE protein. Specifically, these antibodies have the ability to inhibit the binding of RAGE to its various ligands. The antibodies or portions thereof of described in the present application are useful for treating a disease or disorder characterized by or induced by pathophysiological ligands of RAGE, for example missfolded proteins like amyloid β and advanced glycation-end-products.

Abstract translation: 本申请涉及分离的蛋白质，特别是单克隆抗体，特别是结合RAGE蛋白质的CDR-移植的人源化抗体。 具体地说，这些抗体具有抑制RAGE与其各种配体结合的能力。 本申请中描述的抗体或其部分可用于治疗由RAGE的病理生理学配体表征或诱导的疾病或病症，例如错折叠蛋白，如淀粉样蛋白β和晚期糖基化终产物。

公开(公告)号：US20140235476A1

公开(公告)日：2014-08-21

申请号：US14141501

申请日：2013-12-27

Applicant: AbbVie, Inc.

Inventor： Jijie GU ,  Maria Cristina HARRIS ,  Lorenzo BENATUIL ,  Chung-Ming HSIEH

IPC: G01N33/566 ,  C07K16/18

CPC classification number: C07K16/18 ,  C07K16/22 ,  C07K16/244 ,  C07K16/245 ,  C07K2317/31 ,  C07K2317/55 ,  C07K2317/565 ,  C07K2317/626 ,  C07K2317/92 ,  G01N33/6845

Abstract: Provided are protein, nucleic acid, and cellular libraries of multivalent binding proteins (e.g., DVD-Fab or DVD-Ig molecules) and the use of these libraries for the screening of multivalent binding proteins using cell surface display technology (e.g., yeast display).

Abstract translation: 提供了多价结合蛋白（例如，DVD-Fab或DVD-Ig分子）的蛋白质，核酸和细胞文库，以及使用这些文库用于使用细胞表面显示技术（例如酵母展示）筛选多价结合蛋白的方法， 。

公开(公告)号：US20140213770A1

公开(公告)日：2014-07-31

申请号：US14136076

申请日：2013-12-20

Applicant: AbbVie, Inc.

Inventor： Feng DONG ,  Jijie GU ,  Chung-Ming HSIEH

IPC: C12N15/10 ,  C40B30/02 ,  C07K16/00

CPC classification number: C12N15/1089 ,  C07K16/465 ,  C40B30/02

Abstract: The present invention relates to improved methods for antibody engineering, e.g., humanization. In particular, the disclosure provides a high-throughput antibody humanization process that can be automated by computer-implementation.

Abstract translation: 本发明涉及用于抗体工程的改进方法，例如人源化。 具体地，本公开提供了可以通过计算机实现来自动化的高通量抗体人源化过程。

公开(公告)号：US20160031986A1

公开(公告)日：2016-02-04

申请号：US14798849

申请日：2015-07-14

Applicant: AbbVie Inc.

Inventor： Ming-Jiu CHEN ,  Chung-Ming HSIEH ,  Jijie GU ,  Susan E. MORGAN-LAPPE ,  Yingchun LI

IPC: C07K16/28 ,  A61K45/06 ,  A61K39/395

CPC classification number: C07K16/28 ,  A61K39/3955 ,  A61K45/06 ,  A61K2039/505 ,  C07K2317/24 ,  C07K2317/73 ,  C07K2317/76 ,  C07K2317/92

Abstract: DLL4 binding proteins are described herein, including antibodies, CDR-grafted antibodies, humanized antibodies, and DLL4 binding fragments thereof, proteins that bind DLL4 with high affinity, and DLL4 binding proteins that neutralize DLL4 and/or VEGF activity. The DLL4 binding proteins are useful for treating or preventing cancers and tumors and especially for treating or preventing tumor angiogenesis.

Abstract translation: 本文描述了DLL4结合蛋白，包括抗体，CDR-移植抗体，人源化抗体及其DLL4结合片段，以高亲和力结合DLL4的蛋白质，以及中和DLL4和/或VEGF活性的DLL4结合蛋白。 DLL4结合蛋白可用于治疗或预防癌症和肿瘤，特别是用于治疗或预防肿瘤血管生成。

公开(公告)号：US20140199319A1

公开(公告)日：2014-07-17

申请号：US14106116

申请日：2013-12-13

Applicant: AbbVie, Inc.

Inventor： Jane SEAGAL ,  Eve H. BARLOW ,  Chung-Ming HSIEH ,  Jeffrey Yen PAN ,  Shawn M. JENNINGS ,  Mary R. LEDDY ,  Archana B. THAKUR

IPC: C12N5/16 ,  G01N33/569 ,  C07K16/00 ,  C12N15/02

CPC classification number: G01N33/56972 ,  C07K16/00 ,  C07K2317/14 ,  C12N5/163 ,  C12N15/02

Abstract: Disclosed herein are methods for improving the efficiency of the production of hybridomas, e.g., through enrichment of IgG expressing B cells. Also disclosed are hybridoma compositions produced using these methods as well as methods for producing antibodies with the hybridomas.

Abstract translation: 本文公开了用于提高杂交瘤生产效率的方法，例如通过富集表达B细胞的IgG的方法。 还公开了使用这些方法生产的杂交瘤组合物以及用杂交瘤产生抗体的方法。

公开(公告)号：US20140243228A1

公开(公告)日：2014-08-28

申请号：US14141498

申请日：2013-12-27

Applicant: AbbVie, Inc.

Inventor： Lorenzo BENATUIL ,  Jennifer PEREZ ,  Chung-Ming HSIEH

IPC: G01N33/68

CPC classification number: G01N33/6854 ,  C07K16/005 ,  C07K16/18 ,  C07K16/22 ,  C07K16/244 ,  C07K16/245 ,  C07K16/468 ,  C07K2317/64 ,  C07K2317/92 ,  C12N15/1037 ,  C40B20/04 ,  C40B30/04

Abstract: System and methods are disclosed for identifying and isolating antibodies with specific affinity with an antigen of interest. Multiple DNA libraries encoding antibodies or their fragments are designed such that the encoded antibodies from different libraries are tagged differently. These libraries may be transformed into yeast cells. The variants of the antibodies are displayed on the surface of the yeast cells and flow cytometry may be used to sort the cells based on antigen affinity and the different tags on the antibodies. By allowing multiple libraries to be screened simultaneously, the disclosed methods help improve the efficiency of affinity.

Abstract translation: 公开了鉴定和分离与感兴趣的抗原具有特异性亲和力的抗体的系统和方法。 设计编码抗体或其片段的多个DNA文库，使得来自不同文库的编码抗体被不同地标记。 这些文库可以转化成酵母细胞。 抗体的变体显示在酵母细胞的表面上，并且流式细胞术可以用于基于抗体的抗原亲和力和不同标签对细胞进行分类。 通过允许同时筛选多个库，所公开的方法有助于提高亲和力的效率。

公开(公告)号：US20170088611A1

公开(公告)日：2017-03-30

申请号：US15252115

申请日：2016-08-30

Applicant: AbbVie Inc.

Inventor： Lorenzo BENATUIL ,  Chung-Ming HSIEH

IPC: C07K16/24 ,  C12N15/10 ,  C07K16/22

CPC classification number: C07K16/245 ,  C07K16/22 ,  C07K16/241 ,  C07K16/244 ,  C07K16/468 ,  C07K2317/35 ,  C07K2317/56 ,  C07K2317/622 ,  C07K2317/626 ,  C12N15/1082 ,  C40B40/10

Abstract: Provided are protein, nucleic acid, and cellular libraries of single chain multivalent binding proteins (e.g., scDVD and scDVDFab molecules) and methods of using these of these libraries for the screening of single chain multivalent binding proteins using cell surface display technology (e.g., yeast display).

公开(公告)号：US20150344566A1

公开(公告)日：2015-12-03

申请号：US14804834

申请日：2015-07-21

Applicant: AbbVie Inc.

Inventor： Chung-Ming HSIEH ,  Tariq GHAYUR ,  Carrie L. GOODREAU

IPC: C07K16/24 ,  A61K45/06 ,  G01N33/68 ,  A61K39/395

CPC classification number: C07K16/468 ,  A61K39/3955 ,  A61K45/06 ,  C07K16/244 ,  C07K2317/14 ,  C07K2317/24 ,  C07K2317/31 ,  C07K2317/52 ,  C07K2317/56 ,  C07K2317/565 ,  C07K2317/567 ,  C07K2317/64 ,  C07K2317/76 ,  C07K2317/92 ,  G01N33/6854 ,  G01N33/6869 ,  G01N2333/54 ,  G01N2333/5437

Abstract: Engineered multivalent and multispecific binding proteins that bind IL-13 and/or IL-17 are provided, along with methods of making and uses in the prevention, diagnosis, and/or treatment of disease.

Abstract translation: 提供结合IL-13和/或IL-17的工程化多价和多特异性结合蛋白，以及制备和用于预防，诊断和/或治疗疾病的方法。

公开(公告)号：US20140221621A1

公开(公告)日：2014-08-07

申请号：US14141500

申请日：2013-12-27

Applicant: AbbVie, Inc.

Inventor： Lorenzo BENATUIL ,  Chung-Ming HSIEH

IPC: C07K16/46 ,  A61K39/395 ,  A61K47/48

CPC classification number: C07K16/245 ,  C07K16/22 ,  C07K16/241 ,  C07K16/244 ,  C07K16/468 ,  C07K2317/35 ,  C07K2317/56 ,  C07K2317/622 ,  C07K2317/626 ,  C12N15/1082 ,  C40B40/10

Abstract: Provided are protein, nucleic acid, and cellular libraries of single chain multivalent binding proteins (e.g., scDVD and scDVDFab molecules) and methods of using these of these libraries for the screening of single chain multivalent binding proteins using cell surface display technology (e.g., yeast display).

Abstract translation: 提供单链多价结合蛋白（例如scDVD和scDVDFab分子）的蛋白质，核酸和细胞文库以及使用这些文库用于使用细胞表面显示技术筛选单链多价结合蛋白的方法（例如，酵母 显示）。