公开(公告)号：US20190129154A1

公开(公告)日：2019-05-02

申请号：US15942130

申请日：2018-03-30

申请人： AcuSolutions Inc.

发明人： Kuang-Yu HSU ,  Chien-Chung TSAI

IPC分类号： G02B21/04 ,  G02B21/16 ,  G02B21/00 ,  G02B21/06

摘要： An optical sectioning apparatus combining Mirau optical interference microscopy and fluorescence microscopy, including: a wide band light source; an optical circulator, facing the wide band light source for splitting an incident light beam into a reflected light beam and a transmitted light beam; a short wavelength light source; a first dichroic splitter, facing the short wavelength light source and the optical circulator respectively, and being capable of providing a light-blocking effect on a band of wavelengths shorter than a preset wavelength; a Mirau interference objective lens, having a collimated side facing the first dichroic splitter; a sample carrier unit facing a focal side of the Mirau interference objective lens; a projection lens, having a light entrance side facing the optical circulator; and a sensor unit facing a light exit side of the projection lens.

公开(公告)号：US20190302436A1

公开(公告)日：2019-10-03

申请号：US16356727

申请日：2019-03-18

申请人： AcuSolutions Inc.

发明人： Kuang-Yu HSU ,  Chien-Chung TSAI ,  Sey-En LIN

IPC分类号： G02B21/00 ,  G02B21/36 ,  G06T7/90 ,  A01N1/02

摘要： A method for producing image of biological sample, including steps: inputting a grayscale reflection image or a grayscale interference image of a biological sample into a first memory block; inputting a grayscale fluorescent image of the biological sample into a second memory block; using an information processing apparatus to convert the grayscale reflection image or the grayscale interference image into an RGB reflection image or an RGB interference image via first color transform operation and using the information processing apparatus to convert the grayscale fluorescent image into an RGB fluorescent image via second color transform operation; using the information processing apparatus to perform an image fusion operation and an intensity inversion operation on the RGB reflection image and the RGB fluorescent image or on the RGB interference image and the RGB fluorescent image to generate a pseudo H&E image; and outputting the pseudo H&E image to a display unit.

公开(公告)号：US20190353883A1

公开(公告)日：2019-11-21

申请号：US16531086

申请日：2019-08-04

申请人： AcuSolutions Inc.

发明人： Kuang-Yu HSU ,  Chien-Chung TSAI

IPC分类号： G02B21/04 ,  G02B21/00 ,  G02B21/36 ,  G02B21/16 ,  G02B21/06 ,  G01B9/02 ,  G02B21/14

摘要： An optical sectioning apparatus combining Mirau optical interference microscopy and fluorescence microscopy, including: a wide band light source; an optical circulator, facing the wide band light source for splitting an incident light beam into a reflected light beam and a transmitted light beam; a short wavelength light source; a first dichroic splitter, facing the short wavelength light source and the optical circulator respectively, and being capable of providing a light-blocking effect on a band of wavelengths shorter than a preset wavelength; a Mirau interference objective lens, having a collimated side facing the first dichroic splitter; a sample carrier unit facing a focal side of the Mirau interference objective lens; a projection lens, having a light entrance side facing the optical circulator; and a sensor unit facing a light exit side of the projection lens.

公开(公告)号：US20200043202A1

公开(公告)日：2020-02-06

申请号：US16597429

申请日：2019-10-09

申请人： AcuSolutions Inc.

发明人： Chien-Chung TSAI ,  Kuang-Yu HSU ,  Dong-Yo JHENG ,  Sey-En LIN

IPC分类号： G06T11/00 ,  G06T7/90

摘要： A pseudo H&E image producing method, including: inputting a grayscale interference image or a grayscale reflected image of a pathological sample to a first memory block of an information processing apparatus, and inputting a grayscale fluorescence image of the pathological sample to a second memory block of the information processing apparatus; using the information processing apparatus to perform a first color transform operation on the grayscale interference image or the grayscale reflected image to generate a first RGB image, and using the information processing apparatus to perform a second color transform operation on the grayscale fluorescence image to generate a second RGB image; and using the information processing apparatus to perform an image fusion operation and an intensity reversal operation on the first RGB image and the second RGB image to generate a pseudo H&E image. The present invention also discloses an optical system using the method.

公开(公告)号：US20200025744A1

公开(公告)日：2020-01-23

申请号：US16452473

申请日：2019-06-25

申请人： AcuSolutions Inc.

发明人： Chien-Chung TSAI ,  Kuang-Yu HSU ,  Dong-Yo JHENG

IPC分类号： G01N33/483 ,  G02B21/06 ,  G02B21/02 ,  G02B21/16 ,  G02B21/18 ,  G02B27/14 ,  G01N21/64 ,  G02B27/28 ,  G02B21/26 ,  G01N21/45

摘要： An optical sectioning apparatus including: a first white light source unit for generating a first white light beam; a light mixing unit facing the first white light source unit for dividing the first white light beam into a first partial beam and a second partial beam; a reference end unit for making the second partial beam travel a round trip along an adjustable optical path; a first objective lens having a collimated side facing the light mixing unit; a carrier unit facing a focal side of the first objective lens; a projection lens having a light entering side facing the light mixing unit; and a sensing unit facing a light exiting side of the projection lens.

公开(公告)号：US20190028687A1

公开(公告)日：2019-01-24

申请号：US15864323

申请日：2018-01-08

申请人： AcuSolutions Inc.

发明人： Chien-Chung TSAI ,  Kuang-Yu HSU

IPC分类号： H04N9/43 ,  G06T7/00 ,  G06T5/50

CPC分类号： H04N9/43 ,  G01N21/64 ,  G01N21/6458 ,  G06T5/50 ,  G06T7/0014 ,  G06T11/001 ,  G06T2207/10024 ,  G06T2207/10056 ,  G06T2207/10064 ,  G06T2207/20221 ,  G06T2207/30004 ,  G06T2207/30024

摘要： A pseudo H&E image producing method, including: inputting a grayscale interference image or a grayscale reflected image of a pathological sample to a first memory block of an information processing apparatus, and inputting a grayscale fluorescence image of the pathological sample to a second memory block of the information processing apparatus; using the information processing apparatus to perform a first color transform operation on the grayscale interference image or the grayscale reflected image to generate a first RGB image, and using the information processing apparatus to perform a second color transform operation on the grayscale fluorescence image to generate a second RGB image; and using the information processing apparatus to perform an image fusion operation and an intensity reversal operation on the first RGB image and the second RGB image to generate a pseudo H&E image. The present invention also discloses an optical system using the method.

公开(公告)号：US20180347960A1

公开(公告)日：2018-12-06

申请号：US15679478

申请日：2017-08-17

申请人： AcuSolutions Inc.

发明人： Chien-Chung TSAI ,  Kuang-Yu HSU

IPC分类号： G01B9/02 ,  H04N7/18 ,  A61B5/00

CPC分类号： G02B21/16 ,  G01B9/0203 ,  G01B9/02091 ,  G01B2290/70 ,  G02B21/14 ,  G02B21/367

摘要： An optical sectioning apparatus using optical interference microscopy and fluorescence microscopy, including: a beam splitter capable of splitting an incident light beam into a reflected light beam and a transmitted light beam; a wide band light source for providing the incident light beam; a reference arm unit for making the transmitted light beam travel a round trip along an adjustable optical path; a short wavelength light source; a first dichroic splitter, with a first side facing the short wavelength light source and a third side facing the beam splitter, being capable of providing a light-blocking effect on wavelengths shorter than a preset wavelength; an objective lens, with a collimated side facing a second side of the first dichroic splitter; a sample carrier unit facing a focal side of the objective lens; and a projection lens and a sensor units for receiving an output light beam.