公开(公告)号：US11332765B2

公开(公告)日：2022-05-17

申请号：US16931711

申请日：2020-07-17

Applicant: AGENCY FOR SCIENCE, TECHNOLOGY AND RESEARCH

Inventor： Congqiang Zhang ,  Xixian Chen ,  Sudha Shukal

IPC: C12P15/00 ,  C12N9/88 ,  C12N15/70

Abstract: A method of increasing terpenoid production in a host cell that produces one or more terpenoids, comprising: a) providing a host cell that produces one or more terpenoids, said host cell comprising a vector comprising a polynucleotide sequence encoding a terpene synthase enzyme; b) modifying the vector to:
i. introduce an inducible promoter operably linked to the polynucleotide sequence encoding the terpene synthase enzyme; and ii. introduce a polynucleotide sequence encoding a ribosomal binding site (RBS) that increases translation initiation rate of the terpenoid compared to a wild type ribosomal binding site; c) determining the dosage of an inducer capable of inducing the inducible promoter; d) culturing the host cell in a culture medium in the presence of the inducer at the dosage determined from step c); and e) isolating the terpenoid from the culture medium.

公开(公告)号：US20210040520A1

公开(公告)日：2021-02-11

申请号：US16931711

申请日：2020-07-17

Applicant: AGENCY FOR SCIENCE, TECHNOLOGY AND RESEARCH

Inventor： Congqiang Zhang ,  Xixian Chen ,  Sudha Shukal

IPC: C12P15/00 ,  C12N15/70 ,  C12N9/88

Abstract: A method of increasing terpenoid production in a host cell that produces one or more terpenoids, comprising: a) providing a host cell that produces one or more terpenoids, said host cell comprising a vector comprising a polynucleotide sequence encoding a terpene synthase enzyme; b) modifying the vector to:
i. introduce an inducible promoter operably linked to the polynucleotide sequence encoding the terpene synthase enzyme; and ii. introduce a polynucleotide sequence encoding a ribosomal binding site (RBS) that increases translation initiation rate of the terpenoid compared to a wild type ribosomal binding site; c) determining the dosage of an inducer capable of inducing the inducible promoter; d) culturing the host cell in a culture medium in the presence of the inducer at the dosage determined from step c); and e) isolating the terpenoid from the culture medium.

公开(公告)号：US11920137B2

公开(公告)日：2024-03-05

申请号：US16488504

申请日：2018-02-26

Applicant: AGENCY FOR SCIENCE, TECHNOLOGY AND RESEARCH

Inventor： Congqiang Zhang ,  Xixian Chen ,  Heng-Phon Too

IPC: C12N15/52 ,  C12N9/02 ,  C12N9/90 ,  C12N15/62 ,  C12N15/70 ,  C12P5/02 ,  C12P7/26 ,  C12P23/00

CPC classification number: C12N15/52 ,  C12N9/0069 ,  C12N9/90 ,  C12N15/62 ,  C12N15/70 ,  C12P5/026 ,  C12P7/26 ,  C12P23/00 ,  C12Y113/11063 ,  C12Y113/11068 ,  C12Y505/01018 ,  C12Y505/01019

Abstract: A method for producing a carotenoid or apocarotenoid is disclosed. The method comprises the step of expressing in a host cell an expression module comprising an expression vector having a coding region encoding at least one optimised carotenoid or apocarotenoid generating enzyme, the coding region being operably linked to a promoter. A host cell comprising an expression vector having a coding region encoding at least one optimised carotenoid or apocarotenoid generating enzyme, the coding region being operably linked to a promoter is also provided together with a kit.

公开(公告)号：US20210332345A1

公开(公告)日：2021-10-28

申请号：US17272375

申请日：2019-09-02

Applicant: AGENCY FOR SCIENCE, TECHNOLOGY AND RESEARCH ,  JUSTUS-LIEBIG-UNIVERSITAET GIESSEN

Inventor： Congqiang Zhang ,  Xixian Chen ,  Ruehl Martin ,  Heng-Phon Too

IPC: C12N9/88 ,  C12N9/10 ,  C12N9/90 ,  C12P5/00

Abstract: A bacterial strain comprising one or more vectors encoding a) one or more enzymes to produce one or more terpene precursors; and b) a fungal terpene synthase (FTPS). The present invention also relates to a method of producing a terpenoid comprising a) culturing the bacterial strain described herein in an expression medium; and b) isolating the terpenoid from said expression medium.

公开(公告)号：US20240175036A1

公开(公告)日：2024-05-30

申请号：US18384044

申请日：2023-10-26

Applicant: Agency for Science, Technology and Research

Inventor： Congqiang Zhang ,  Xixian Chen ,  Heng-Phon Too

IPC: C12N15/52 ,  C12N9/02 ,  C12N9/90 ,  C12N15/62 ,  C12N15/70 ,  C12P5/02 ,  C12P7/26 ,  C12P23/00

CPC classification number: C12N15/52 ,  C12N9/0069 ,  C12N9/90 ,  C12N15/62 ,  C12N15/70 ,  C12P5/026 ,  C12P7/26 ,  C12P23/00 ,  C12Y113/11063 ,  C12Y113/11068 ,  C12Y505/01018 ,  C12Y505/01019

Abstract: A method for producing a carotenoid or apocarotenoid is disclosed. The method comprises the step of expressing in a host cell an expression module comprising an expression vector having a coding region encoding at least one optimised carotenoid or apocarotenoid generating enzyme, the coding region being operably linked to a promoter. A host cell comprising an expression vector having a coding region encoding at least one optimised carotenoid or apocarotenoid generating enzyme, the coding region being operably linked to a promoter is also provided together with a kit.

公开(公告)号：US11680256B2

公开(公告)日：2023-06-20

申请号：US17272375

申请日：2019-09-02

Applicant: AGENCY FOR SCIENCE, TECHNOLOGY AND RESEARCH ,  JUSTUS-LIEBIG-UNIVERSITAET GIESSEN

Inventor： Congqiang Zhang ,  Xixian Chen ,  Ruehl Martin ,  Heng-Phon Too

IPC: C12N9/88 ,  C12N9/10 ,  C12N9/90 ,  C12P5/00 ,  C12N1/21

CPC classification number: C12N9/88 ,  C12N9/1022 ,  C12N9/90 ,  C12P5/002 ,  C12Y202/01007 ,  C12Y503/03002

Abstract: A bacterial strain comprising one or more vectors encoding a) one or more enzymes to produce one or more terpene precursors; and b) a fungal terpene synthase (FTPS). The present invention also relates to a method of producing a terpenoid comprising a) culturing the bacterial strain described herein in an expression medium; and b) isolating the terpenoid from said expression medium.