公开(公告)号：US20240110241A1

公开(公告)日：2024-04-04

申请号：US18256303

申请日：2021-12-07

Applicant: AGENCY FOR SCIENCE, TECHNOLOGY AND RESEARCH

Inventor： Lai Guan Ng ,  Shu Feng Melissa Ng ,  De Li Leonard Tan ,  Florent Benoit Claude Ginhoux

IPC: C12Q1/6881

CPC classification number: C12Q1/6881 ,  C12Q2600/158 ,  G01N2800/52 ,  G01N2800/56

Abstract: The invention relates to a method of detecting and/or characterising an immunosuppressive neutrophil comprising determining and/or measuring the expression of one or more anti-apoptotic markers in a neutrophil population. In an embodiment, the immunosuppressive neutrophil is a polymorphonuclear myeloid-derived suppressor cell (PMNMDSC) and the anti-apoptotic marker is TNF-related apoptosis-inducing ligand (TRAIL)-receptor 3 (TRAILR3/TNFRSF10C) and/or decoy TNF-related apoptosis-inducing ligand (TRAIL)-receptor (dcTRAILR1). Also disclosed are methods of evaluating the progression of a proliferative disease in a subject, methods of evaluating the efficacy of a treatment regimen for a proliferative disease in a subject, methods of treating a proliferative disease in a subject, and kits thereof.

公开(公告)号：US20230213513A1

公开(公告)日：2023-07-06

申请号：US18000559

申请日：2021-06-11

Applicant: Agency for Science, Technology and Research

Inventor： Lai Guan Ng ,  Immanuel Weng Han Kwok ,  De Li Leonard Tan ,  Florent Ginhoux

IPC: G01N33/569

CPC classification number: G01N33/56972 ,  G01N2333/705

Abstract: There is provided a method of identifying a neutrophil progenitor, the method comprising: determining an expression of at least one biomarker selected from the group consisting of: CD71, LOX-1, CD164, CD112, CD181, TACSTD2, CD11b and CD49d in a cell. In various embodiments, the cell is identified as a neutrophil progenitor when it is determined to have at least one of the following expression profiles: CD71hi/+, LOX-1int/lo/−, CD164hi/+, CD112hi/+, CD181int/lo/−, TACSTD2hi/+, CD11blo/− and/or CD49dint/hi/+. Also disclosed are a method of sorting and/or separating neutrophil progenitors from a cell population, a composition that is enriched in neutrophil progenitors and related uses and methods.

公开(公告)号：US12072335B2

公开(公告)日：2024-08-27

申请号：US17117455

申请日：2020-12-10

Applicant: Agency for Science, Technology and Research

Inventor： Guillaume Robert Carissimo ,  Immanuel Kwok Weng Han ,  Weili Xu ,  Lisa Fong Poh Ng ,  Laurent Renia ,  Anis Larbi ,  Lai Guan Ng

IPC: G01N33/569 ,  A61K49/00 ,  G01N33/50

CPC classification number: G01N33/56972 ,  A61K49/0004 ,  G01N33/5094 ,  G01N2333/165 ,  G01N2800/26 ,  G01N2800/50 ,  G01N2800/52 ,  G01N2800/56 ,  G01N2800/60

Abstract: There is provided a method of evaluating risk of severe outcome of an infectious disease and/or an inflammatory disease in a patient, the method comprising: determining/measuring the number of one or more immune cells selected from the group consisting of VD2 T cells, CD8 T cells, and immature neutrophils in a sample obtained from the patient, wherein the patient has an enhanced risk of severe infectious disease and/or inflammatory disease outcome when: (i) the ratio of immature neutrophils to VD2 T cell is at least 2:1, and/or (ii) the ratio of immature neutrophils to CD8 T cell is at least 0.5:1. Also disclosed are method of treating a patient with a severe infectious disease and/or inflammatory disease and kit for use in methods thereof.