公开(公告)号：US20240018490A1

公开(公告)日：2024-01-18

申请号：US18464955

申请日：2023-09-11

申请人： Agilent Technologies, Inc.

发明人： Michelle Cayouette ,  Jeffrey Fox ,  Connie Hansen ,  Holly Hogrefe ,  Weidong Wu

IPC分类号： C12N9/12 ,  C12Q1/6869 ,  C12P19/34

CPC分类号： C12N9/1252 ,  C12Y207/07007 ,  C12Q1/6869 ,  C12P19/34

摘要： Mutant polymerases are provided that have improved ability to incorporate modified nucleotides, including 3′-OH unblocked reversible terminators. The mutant polymerases may be used in a variety of applications, such as for polynucleotide sequencing, primer extension reactions, and template-independent enzymatic oligonucleotide synthesis.

公开(公告)号：US11001886B2

公开(公告)日：2021-05-11

申请号：US16020190

申请日：2018-06-27

申请人： AGILENT TECHNOLOGIES, INC.

发明人： Brian P. Stupi ,  Hong Li ,  Weidong Wu ,  Megan N. Hersh ,  David Hertzog ,  Sidney E. Morris ,  Michael L. Metzker

IPC分类号： C07H19/073 ,  C07H19/10 ,  C07H19/14 ,  C07H19/173 ,  C07H19/20 ,  C12Q1/6869 ,  C12Q1/6883 ,  B01L3/00 ,  C12Q1/6874

摘要： The present invention relates generally to 3′-OH unblocked nucleotides and nucleosides labeled and unlabeled with 5-methoxy-substituted nitrobenzyl-based photocleavable terminating groups for use in methods and systems related to DNA and RNA sequencing and analysis. These compounds may be used as reversible terminators as they exhibit fast nucleotide incorporation kinetics, single-base termination, high nucleotide selectivity, and rapid terminating group cleavage that results in a naturally occurring nucleotide.

公开(公告)号：US20240294886A1

公开(公告)日：2024-09-05

申请号：US18652554

申请日：2024-05-01

申请人： Agilent Technologies, Inc.

发明人： Michelle Cayouette ,  Jeffrey Fox ,  Connie Hansen ,  Holly Hogrefe ,  Weidong Wu

IPC分类号： C12N9/12 ,  C12P19/34 ,  C12Q1/6869

CPC分类号： C12N9/1252 ,  C12P19/34 ,  C12Q1/6869 ,  C12Y207/07007

摘要： Mutant polymerases are provided that have improved ability to incorporate modified nucleotides, including 3′—OH unblocked reversible terminators. The mutant polymerases may be used in a variety of applications, such as for polynucleotide sequencing, primer extension reactions, and template-independent enzymatic oligonucleotide synthesis.

公开(公告)号：US10942176B2

公开(公告)日：2021-03-09

申请号：US15230876

申请日：2016-08-08

申请人： AGILENT TECHNOLOGIES, INC.

发明人： Mimi Healy ,  Xiyi Chen ,  Jinchun Wang ,  Weidong Wu ,  Sophia Petrichenko ,  Su Zhang ,  Stacie Frye

IPC分类号： G01N33/543 ,  G01N33/53 ,  G01N33/58 ,  G01N33/533 ,  B01L3/00 ,  G01N1/30 ,  G01N1/31 ,  G01N35/00

摘要： Provided herein are methods of using photocleavable labels for multiplex and serial antigen detection. The methods comprise detecting the presence of photocleavable labels, which are conjugated through functional linkers to antigen-binding complexes, which in turn non-covalently bind to antigens. The presence of a photocleavable label is indicative of the presence of an antigen specifically or selectively bound by an antigen-binding complex. Also provided are apparatuses for using photocleavable labels for multiplex and serial antigen detection.

公开(公告)号：US11773380B1

公开(公告)日：2023-10-03

申请号：US17894854

申请日：2022-08-24

申请人： Agilent Technologies, Inc.

发明人： Michelle Cayouette ,  Jeffrey Fox ,  Connie Hansen ,  Holly Hogrefe ,  Weidong Wu

IPC分类号： C12N9/12 ,  C12P19/34 ,  C12Q1/6869

CPC分类号： C12N9/1252 ,  C12P19/34 ,  C12Q1/6869 ,  C12Y207/07007

摘要： Mutant polymerases are provided that have improved ability to incorporate modified nucleotides, including 3′-OH unblocked reversible terminators. The mutant polymerases may be used in a variety of applications, such as for polynucleotide sequencing, primer extension reactions, and template-independent enzymatic oligonucleotide synthesis.

公开(公告)号：US20230126241A1

公开(公告)日：2023-04-27

申请号：US17914700

申请日：2021-02-03

申请人： Agilent Technologies, Inc.

发明人： Jinchun Wang ,  Jurgen Del Favero ,  Weidong Wu ,  Holly Hogrefe

IPC分类号： C12Q1/686 ,  C12Q1/6876

摘要： Methods and compositions are described for multi-stage primer extension reactions such as multiplex polymerase chain reactions (PCR) and reverse transcriptase PCR. Primer extension stages are performed in a closed vessel without opening the vessel between stages. The multi-stage primer extension methods and compositions utilize earlier stage primers in an earlier stage and later stage primers in a later stage, wherein the later stage primers are blocked from extension during the earlier stage. The blocked primers of the present technology comprise photocleavable blocking groups and are substantially inactive until the blocking group is cleaved by exposure to ultraviolet light. The blocked primers can be activated by ultraviolet light without opening the vessel.

公开(公告)号：US12006519B2

公开(公告)日：2024-06-11

申请号：US18464955

申请日：2023-09-11

申请人： Agilent Technologies, Inc.

发明人： Michelle Cayouette ,  Jeffrey Fox ,  Connie Hansen ,  Holly Hogrefe ,  Weidong Wu

IPC分类号： C12N9/12 ,  C12P19/34 ,  C12Q1/6869

CPC分类号： C12N9/1252 ,  C12P19/34 ,  C12Q1/6869 ,  C12Y207/07007

摘要： Mutant polymerases are provided that have improved ability to incorporate modified nucleotides, including 3′-OH unblocked reversible terminators. The mutant polymerases may be used in a variety of applications, such as for polynucleotide sequencing, primer extension reactions, and template-independent enzymatic oligonucleotide synthesis.