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公开(公告)号：US20110151567A1
公开(公告)日：2011-06-23
申请号：US12530135
申请日：2008-04-08
申请人： Shenghao Liu , Keiji Endo , Katsutoshi Ara
发明人： Shenghao Liu , Keiji Endo , Katsutoshi Ara
CPC分类号： C12P21/02
摘要： A recombinant microorganism having improved productivity for a protein or a polypeptide, and a method for producing a protein or a polypeptide using the recombinant microorganism, are provided. A recombinant microorganism obtained by transfecting a gene for encoding a desired protein or polypeptide into a microorganism strain which is obtained by genetically constructing to overexpress secY gene of Bacillus subtilis or a gene corresponding to the secY gene, and deleting or inactivating one or more genes selected from sporulation-associated genes and genes corresponding to the sporulation-associated genes from the genome.
摘要翻译： 提供了具有提高的蛋白质或多肽的生产力的重组微生物，以及使用该重组微生物生产蛋白质或多肽的方法。 将通过将所需蛋白质或多肽编码的基因转染到通过遗传构建以过表达枯草芽孢杆菌的secY基因或相应于所述secY基因的基因而获得的微生物菌株获得的重组微生物，以及删除或灭活所选择的一种或多种基因 来自与来自基因组的孢子形成相关基因相对应的孢子形成相关基因和基因。
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2.发明授权Recombinant microorganism that expresses a secY gene with deletion of sporulation-associated genes and method of producing thereof 有权表达具有孢子形成相关基因缺失的secY基因的重组微生物及其制备方法公开(公告)号：US08389264B2
公开(公告)日：2013-03-05
申请号：US12530135
申请日：2008-04-08
申请人： Shenghao Liu , Keiji Endo , Katsutoshi Ara
发明人： Shenghao Liu , Keiji Endo , Katsutoshi Ara
IPC分类号： C12N1/21
CPC分类号： C12P21/02
摘要： A recombinant microorganism having improved productivity for a protein or a polypeptide, and a method for producing a protein or a polypeptide using the recombinant microorganism, are provided.A recombinant microorganism obtained by transfecting a gene for encoding a desired protein or polypeptide into a microorganism strain which is obtained by genetically constructing to overexpress secY gene of Bacillus subtilis or a gene corresponding to the secY gene, and deleting or inactivating one or more genes selected from sporulation-associated genes and genes corresponding to the sporulation-associated genes from the genome.
摘要翻译： 提供了具有提高的蛋白质或多肽的生产力的重组微生物，以及使用该重组微生物生产蛋白质或多肽的方法。 将通过将所需蛋白质或多肽编码的基因转染到通过遗传构建以过表达枯草芽孢杆菌的secY基因或相应于所述secY基因的基因而获得的微生物菌株获得的重组微生物，以及删除或灭活所选择的一种或多种基因 来自与来自基因组的孢子形成相关基因相对应的孢子形成相关基因和基因。
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公开(公告)号：US20090029417A1
公开(公告)日：2009-01-29
申请号：US12279271
申请日：2007-02-15
申请人： Keiji Endo , Shenghao Liu , Katsutoshi Ara
发明人： Keiji Endo , Shenghao Liu , Katsutoshi Ara
CPC分类号： C12N9/2437
摘要： The object of the invention is to provide a microorganism which enhances productivity of cellulase and is useful for industrial production of cellulase, and to provide a method for producing cellulase by use of the microorganism. The present invention provides a recombinant microorganism produced by transferring a gene encoding cellulase to a parental microorganism which has been genetically modified so as to overexpress a Bacillus subtilis secY gene or a gene corresponding thereto.
摘要翻译： 本发明的目的是提供一种提高纤维素酶生产率并可用于纤维素酶的工业生产的微生物，并提供使用微生物生产纤维素酶的方法。 本发明提供了一种通过将编码纤维素酶的基因转移到已被遗传修饰以使其过表达枯草芽孢杆菌SecY基因或相应基因的亲本微生物而产生的重组微生物。
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4.发明授权公开(公告)号：US08460893B2
公开(公告)日：2013-06-11
申请号：US12279271
申请日：2007-02-15
申请人： Keiji Endo , Shenghao Liu , Katsutoshi Ara
发明人： Keiji Endo , Shenghao Liu , Katsutoshi Ara
CPC分类号： C12N9/2437
摘要： The object of the invention is to provide a microorganism which enhances productivity of cellulase and is useful for industrial production of cellulase, and to provide a method for producing cellulase by use of the microorganism. The present invention provides a recombinant microorganism produced by transferring a gene encoding cellulase to a parental microorganism which has been genetically modified so as to overexpress a Bacillus subtilis secY gene or a gene corresponding thereto.
摘要翻译： 本发明的目的是提供一种提高纤维素酶生产率并可用于纤维素酶的工业生产的微生物，并提供使用微生物生产纤维素酶的方法。 本发明提供了一种通过将编码纤维素酶的基因转移到已被遗传修饰以使其过表达枯草芽孢杆菌SecY基因或相应基因的亲本微生物而产生的重组微生物。
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公开(公告)号：US20100255534A1
公开(公告)日：2010-10-07
申请号：US12528039
申请日：2007-12-27
申请人： Keiji Endo , Katsutoshi Ara
发明人： Keiji Endo , Katsutoshi Ara
摘要： Provision of a recombinant microorganism which has increased productivity of a protein or polypeptide of interest and a method for producing a protein or polypeptide of interest using the recombinant microorganism. The recombinant microorganism is produced by transferring a gene encoding a protein or polypeptide of interest to a microorganism strain, wherein the microorganism strain is prepared by: introducing a transcription initiation regulatory region that functions in the microorganism or both the transcription initiation regulatory region and a ribosome-binding site that functions in the microorganism into the upstream of a Bacillus subtilis prsA gene or a gene corresponding thereto in the genome of a parental microorganism, or introducing a gene fragment prepared by ligating a transcription initiation regulatory region that functions in the microorganism or both the transcription initiation regulatory region and a ribosome-binding site that functions in the microorganism to the upstream of the Bacillus subtilis prsA gene or a gene corresponding thereto into the genome of a parental microorganism; and deleting or inactivating one or more genes selected from an abrB gene, a dltA gene, a dltB gene, a dltC gene, a dltD gene, a dltE gene, and a gene (genes) corresponding thereto.
摘要翻译： 提供具有增加的目的蛋白质或多肽的生产力的重组微生物以及使用重组微生物产生感兴趣的蛋白质或多肽的方法。 通过将编码目的蛋白质或多肽的基因转移到微生物菌株来产生重组微生物，其中微生物菌株通过以下方式制备：引入在微生物中起作用的转录起始调节区或转录起始调节区和核糖体 在微生物中起作用的枯草芽孢杆菌prsA基因或与亲本微生物基因组相对应的基因的上游的引入位点，或引入通过连接在微生物中起作用的转录起始调节区或两者兼有的基因片段 转录起始调节区和在微生物中起作用的枯草芽孢杆菌prsA基因上游的核糖体结合位点或与之相对应的基因转化为亲本微生物的基因组; 以及删除或失活一个或多个选自abrB基因，dltA基因，dltB基因，dltC基因，dltD基因，dltE基因和与其相对应的基因（基因）的基因。
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公开(公告)号：US07981659B2
公开(公告)日：2011-07-19
申请号：US12083539
申请日：2006-09-25
IPC分类号： C12N1/20
CPC分类号： C12N15/75 , C12N9/2417 , C12N9/54 , C12P21/02
摘要： Novel Bacillus subtilis mutant strains having good productivity of various enzymes are provided through extensive analysis of strains that are derived from Bacillus subtilis via gene disruption. The Bacillus subtilis mutant strains according to the present invention have genomic structures prepared by deletion of regions listed in the columns for deficient regions. Each of these Bacillus subtilis mutant strains exerts significantly improved secretory productivity of a protein when a gene encoding such a secretory target protein is introduced so that it can be expressed, compared with a case in which the same gene is introduced into a wild-type strain.
摘要翻译： 通过对通过基因破坏衍生自枯草芽孢杆菌的菌株的广泛分析，提供具有各种酶的良好生产力的新型枯草芽孢杆菌突变菌株。 根据本发明的枯草芽孢杆菌突变菌株具有通过缺失缺陷区列中列出的区域而制备的基因组结构。 当与将相同基因导入野生型菌株的情况相比，当引入编码这种分泌型靶蛋白的基因以使其能够被表达时，这些枯草芽孢杆菌突变菌株中的每一种都能显着提高蛋白质的分泌生产力 。
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公开(公告)号：US08293516B2
公开(公告)日：2012-10-23
申请号：US12528039
申请日：2007-12-27
申请人： Keiji Endo , Katsutoshi Ara
发明人： Keiji Endo , Katsutoshi Ara
摘要： A recombinant microorganism is provided that has increased productivity of a protein or polypeptide of interest. A method for producing a protein of interest using the microorganism is also provided. The microorganism is prepared by inserting, in its genome, a transcription initiation regulatory region or both a transcription initiation regulatory region and a ribosome-binding site, upstream of a Bacillus subtilis prsA gene and by deleting or inactivating one or more of the abrB gene, dltA gene, dltB gene, dltC gene, dltD gene, dltE gene, or a gene (genes) corresponding thereto.
摘要翻译： 提供了具有增加的感兴趣的蛋白质或多肽的生产力的重组微生物。 还提供了使用该微生物生产感兴趣的蛋白质的方法。 通过在其基因组中插入转录起始调节区或在枯草芽孢杆菌prsA基因的上游插入转录起始调节区和核糖体结合位点，以及通过缺失或失活一个或多个abrB基因， dltA基因，dltB基因，dltC基因，dltD基因，dltE基因或与其对应的基因（基因）。
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公开(公告)号：US20090221055A1
公开(公告)日：2009-09-03
申请号：US12083539
申请日：2006-09-25
IPC分类号： C12N1/21
CPC分类号： C12N15/75 , C12N9/2417 , C12N9/54 , C12P21/02
摘要： Novel Bacillus subtilis mutant strains having good productivity of various enzymes are provided through extensive analysis of strains that are derived from Bacillus subtilis via gene disruption. The Bacillus subtilis mutant strains according to the present invention have genomic structures prepared by deletion of regions listed in the columns for deficient regions. Each of these Bacillus subtilis mutant strains exerts significantly improved secretory productivity of a protein when a gene encoding such a secretory target protein is introduced so that it can be expressed, compared with a case in which the same gene is introduced into a wild-type strain.
摘要翻译： 通过对通过基因破坏衍生自枯草芽孢杆菌的菌株的广泛分析，提供具有各种酶的良好生产力的新型枯草芽孢杆菌突变菌株。 根据本发明的枯草芽孢杆菌突变菌株具有通过缺失缺陷区列中列出的区域而制备的基因组结构。 当与将相同基因导入野生型菌株的情况相比，当引入编码这种分泌型靶蛋白的基因以使其能够被表达时，这些枯草芽孢杆菌突变菌株中的每一种都能显着提高蛋白质的分泌生产力 。
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公开(公告)号：US20090317346A1
公开(公告)日：2009-12-24
申请号：US12548998
申请日：2009-08-27
申请人： Syunichi Akiba , Katsutoshi Ara , Hiroshi Kusuoku , Toyoki Hagura
发明人： Syunichi Akiba , Katsutoshi Ara , Hiroshi Kusuoku , Toyoki Hagura
CPC分类号： A61K36/756 , A61K8/97 , A61K36/16 , A61Q15/00 , A61K2300/00
摘要： This invention relates to deodorant agents, which contain ginkgo or Phellodendron Bark or its extract as an active ingredient.According to the present invention, deodorant agents which are high in safety and can radically inhibit the emission of human body malodors typified by sweat odor, especially, axillary odor.
摘要翻译： 本发明涉及含有银杏或黄柏树皮或其提取物作为活性成分的除臭剂。 根据本发明，安全性高，可以根本上抑制由汗水气味，特别是腋臭引起的人体恶臭的排放的除臭剂。
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公开(公告)号：US20080050807A1
公开(公告)日：2008-02-28
申请号：US10829331
申请日：2004-04-22
申请人： Yuji Hatada , Katsuya Ozaki , Katsutoshi Ara , Shuji Kawai , Susumu Ito
发明人： Yuji Hatada , Katsuya Ozaki , Katsutoshi Ara , Shuji Kawai , Susumu Ito
CPC分类号： C12N9/2417
摘要： The present invention provides a DNA fragment encoding alkaline liquefying α-amylase, recombinant DNA containing the DNA fragment, a transformed microorganism harboring the recombinant DNA, as well as a method for producing alkaline liquefying α-amylase using the transformant. The method of the present invention enables mass production of alkaline liquefying α-amylase useful as a detergent component.The present invention is directed to a liquefying alkaline alphα-amylase, and a DNA encoding for the same and functional fragments thereof.
摘要翻译： 本发明提供了编码碱液化α-淀粉酶的DNA片段，含有DNA片段的重组DNA，携带重组DNA的转化微生物以及使用该转化体产生碱液化α-淀粉酶的方法。 本发明的方法能够大量生产用作洗涤剂组分的碱性液化α-淀粉酶。 本发明涉及液化碱性α-淀粉酶和编码其相同功能片段的DNA。
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