公开(公告)号：US5741652A

公开(公告)日：1998-04-21

申请号：US536740

申请日：1995-09-29

申请人： Akitaka Shibuya ,  Shunji Saitoh ,  Toshio Takahashi ,  Masanori Kamei ,  Naoko Maruo

发明人： Akitaka Shibuya ,  Shunji Saitoh ,  Toshio Takahashi ,  Masanori Kamei ,  Naoko Maruo

IPC分类号： G01N33/53 ,  C07K16/00 ,  C07K16/18 ,  C07K19/00 ,  C12N5/10 ,  C12N15/02 ,  C12P21/08 ,  G01N33/577 ,  G01N33/68

CPC分类号： C07K16/18 ,  G01N33/6887 ,  Y10S435/975

摘要： A method for one-step immunoassay of human type IV collagen, which uses a carrier immobilizing monoclonal antibody against human type IV collagen and a labelled polyclonal antibody against human type IV collagen, as well as a kit therefor and monoclonal antibodies which are particularly suited therefor. It is possible to accurately and quickly measure human type IV collagen in blood serum, for example, and obtain measurement results which accurately reflect pathological conditions.

摘要翻译： 使用针对人IV型胶原的针对人IV型胶原的载体固定化单克隆抗体和针对人IV型胶原的标记多克隆抗体的人IV型胶原的一步免疫测定方法及其特别适用的单克隆抗体 。 例如，可以准确，快速地测量血清中的IV型胶原，并获得准确反映病理状况的测定结果。

公开(公告)号：US6060255A

公开(公告)日：2000-05-09

申请号：US164461

申请日：1998-10-01

申请人： Akitaka Shibuya ,  Shunji Saitoh ,  Toshio Takahashi ,  Naoko Maruo

发明人： Akitaka Shibuya ,  Shunji Saitoh ,  Toshio Takahashi ,  Naoko Maruo

IPC分类号： C07K14/78 ,  C07K16/18 ,  G01N33/68 ,  G01N33/53

CPC分类号： C07K16/18 ,  C07K14/78 ,  G01N33/6887 ,  G01N2333/78 ,  Y10S435/975 ,  Y10S530/846

摘要： A type IV collagen high molecular form having a higher molecular weight than the 7S domain of type IV collagen and including the 7S domain in its structure, is obtained from the supernatant being recovered from a collagen solution digested by pepsin in the following steps;1) salt precipitating with sodium chloride at a concentration no higher than 1.2 M,2) dissolving the precipitates,3) salt precipitating with sodium chloride at a concentration no higher than previous concentration. By reacting a sample with an antibody which reacts with this form, the type IV collagen high molecular form in the sample can be measured to allow diagnosis of the degree of liver fibrosis in patients with liver diseases.

摘要翻译： 从以下步骤从胃蛋白酶消化的胶原溶液中回收的上清液获得具有比IV型胶原的7S结构域更高分子量并包含其结构的7S结构域的IV型胶原高分子形式; 1）浓度不高于1.2M的氯化钠沉淀，2）溶解沉淀物，3）以不高于先前浓度的浓度用氯化钠沉淀。 通过使样品与与该形式反应的抗体反应，可以测量样品中的IV型胶原蛋白高分子形式，以诊断肝脏疾病患者的肝纤维化程度。

公开(公告)号：US06258552B1

公开(公告)日：2001-07-10

申请号：US08603121

申请日：1996-02-20

申请人： Masataka Shiraki ,  Jui-Tung Chen ,  Ikuo Morita ,  Naoko Maruo

发明人： Masataka Shiraki ,  Jui-Tung Chen ,  Ikuo Morita ,  Naoko Maruo

IPC分类号： G01N3353

CPC分类号： G01N33/6869

摘要： A method for estimation of change in bone mineral density or a method for diagnosis of osteoporosis, comprising the step of measuring change in a concentration of soluble interleukin-6 receptor in a blood sample, by for example, sandwich assay or competition assay; and a kit for carrying out the methods, comprising: (1) an anti-sIL-6R antibody immobilized to a solid carrier, and (2) an anti-sIL-6R antibody bound to a detectable marker or capable of binding to a detectable marker.

摘要翻译： 一种评价骨矿物质密度变化的方法或骨质疏松症诊断方法，其特征在于，包括测定血液样品中可溶性白细胞介素-6受体浓度的变化的步骤，例如，夹心测定法，竞赛法等。 以及用于进行所述方法的试剂盒，其包括：（1）固定在固体载体上的抗-SIL-6R抗体，和（2）结合可检测标记或能够结合可检测标记的抗sIL-6R抗体 标记。

公开(公告)号：US6008005A

公开(公告)日：1999-12-28

申请号：US603121

申请日：1996-02-20

申请人： Masataka Shiraki ,  Jui-Tung Chen ,  Ikuo Morita ,  Naoko Maruo

发明人： Masataka Shiraki ,  Jui-Tung Chen ,  Ikuo Morita ,  Naoko Maruo

IPC分类号： G01N33/50 ,  C12P21/08 ,  G01N33/53 ,  G01N33/68 ,  C07K16/00 ,  G01N21/76

CPC分类号： G01N33/6869

摘要： A method for estimation of change in bone mineral density or a method for diagnosis of osteoporosis, comprising the step of measuring change in a concentration of soluble interleukin-6 receptor in a blood sample, by for example, sandwich assay or competition assay; and a kit for carrying out said methods, comprising:(1) an anti-sIL-6R antibody immobilized to a solid carrier, and(2) an anti-sIL-6R antibody is bound to a detectable marker or capable of binding to a detectable marker.

摘要翻译： 一种评价骨矿物质密度变化的方法或骨质疏松症诊断方法，其特征在于，包括测定血液样品中可溶性白细胞介素-6受体浓度的变化的步骤，例如，夹心测定法，竞赛法等。 以及用于实施所述方法的试剂盒，其包含：（1）固定在固体载体上的抗-SIL-6R抗体，和（2）抗-SIL-6R抗体与可检测标记结合或能够结合至 可检测标记。

公开(公告)号：US5932480A

公开(公告)日：1999-08-03

申请号：US816326

申请日：1997-03-13

申请人： Naoko Maruo ,  Masuo Inoue

发明人： Naoko Maruo ,  Masuo Inoue

IPC分类号： G01N33/53 ,  G01N33/531 ,  G01N33/543 ,  G01N33/72

CPC分类号： G01N33/54306 ,  G01N33/72 ,  Y10S435/81 ,  Y10S435/96 ,  Y10S435/975 ,  Y10S436/808

摘要： A sample containing hemoglobin A1c (HbA1c) is simultaneously brought in contact with a solid phase and anti-HbA1c antibody, the anti-HbA1c antibody bound to the adsorbed HbA1c on the solid phase and the anti-HbA1c antibody in solution are separated, and the anti-HbA1c antibody bound to the adsorbed HbA1c on the solid phase is detected. HbA1c % is determined by one-step immunoassay, and the time required for measurement is shortened in comparison with conventional immunoassay methods. Since the exposure time to the pretreatment solution present in the reaction solution during the immune reaction is shortened, formation of a precipitate in the sample as well as inactivation of the enzyme of the enzyme-labeled anti-HbA1c antibody, when used, is reduced.

摘要翻译： 将含有血红蛋白A1c（HbA1c）的样品同时与固相和抗HbA1c抗体接触，将与固相上吸附的HbA1c结合的抗HbA1c抗体和溶液中的抗HbA1c抗体分离， 检测到与固相上吸附的HbA1c结合的抗HbA1c抗体。 HbA1c％通过一步免疫测定法测定，与常规免疫测定方法相比，测定所需的时间缩短。 由于在免疫反应中存在于反应溶液中的预处理溶液的暴露时间缩短，所以在使用时，样品中的沉淀物的形成以及酶标记的抗HbA1c抗体的酶的失活减少。