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    Assays for nuclear receptor agonists and antagonists using fluorescence resonance energy transfer
    5.
    发明授权
    Assays for nuclear receptor agonists and antagonists using fluorescence resonance energy transfer 失效
    使用荧光共振能量转移的核受体激动剂和拮抗剂的测定

    公开(公告)号：US06689574B1

    公开(公告)日：2004-02-10

    申请号：US09166265

    申请日：1998-10-05

    申请人： Richard T. Cummings ,  David E. Moller ,  Jeffrey D. Hermes ,  Gaochao Zhou

    发明人： Richard T. Cummings ,  David E. Moller ,  Jeffrey D. Hermes ,  Gaochao Zhou

    IPC分类号： G01N3353

    CPC分类号： G01N33/566 ,  G01N33/542 ,  G01N2333/70567

    摘要： Provided is a method of identifying agonists and antagonists of nuclear receptors that comprises measuring agonist-dependent fluorescence resonance energy transfer (FRET) between a fluorescently labeled nuclear receptor or ligand binding domain and fluorescently labeled CREB-binding protein (CBP), p300, other nuclear co-activator, or binding portion thereof. The method is simple, rapid, and inexpensive. Nuclear receptors and nuclear receptor co-activators labeled with fluorescent reagents for use in the above-described method are also provided.

    摘要翻译： 提供了一种鉴定核受体的激动剂和拮抗剂的方法，其包括测量荧光标记的核受体或配体结合结构域和荧光标记的CREB结合蛋白（CBP）之间的激动剂依赖性荧光共振能量转移（FRET），p300，其他核 共活化剂或其结合部分。 该方法简单，快速，便宜。 还提供了用于上述方法的用荧光试剂标记的核受体和核受体共激活剂。