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公开(公告)号：US5298599A
公开(公告)日：1994-03-29
申请号：US816679
申请日：1992-01-03
IPC分类号： C12N15/09 , A61K38/00 , C07K1/107 , C07K14/47 , C07K14/745 , C07K16/40 , C07K19/00 , C12N9/64 , C12P21/02 , C12R1/91 , G01N33/68 , G01N33/86 , C07K13/00
CPC分类号： C07K1/107 , C07K14/745 , C07K14/7455 , C07K16/40 , C12N9/6464 , C12Y304/21069 , G01N33/68 , G01N33/86 , A61K38/00 , C07K2319/00 , C07K2319/034 , C07K2319/50 , G01N2333/745 , G01N2333/96447 , G01N2405/04
摘要： A method is disclosed to make any protein in a form that can be isolated rapidly from a solution using a specific monoclonal antibody designated "HPC-4". It has now been determined that it is possible to form a fusion protein of the epitope with a protein to be isolated, and isolate the protein using HPC-4-based affinity chromatography. In the preferred embodiment, a specific protease cleavage site is inserted between the epitope and the protein so that the epitope can be easily removed from the isolated protein. In an example, a functionally active soluble tissue factor including the twelve amino acid epitope recognized in combination with calcium by HPC-4 and a factor Xa cleavage site was expressed from a vector inserted into a procaryotic expression system. The recombinant tissue factor can be rapidly isolated in a single chromatographic step using the HPC-4 monoclonal antibody immobilized on a suitable substrate. Once isolated, the Protein C epitope is removed by cleavage with factor Xa, leaving the functionally active, soluble tissue factor.
摘要翻译： 公开了一种制备任何蛋白质形式的蛋白质，其可以使用指定为“HPC-4”的特异性单克隆抗体从溶液中快速分离。 现在已经确定可以形成表位与待分离的蛋白质的融合蛋白，并使用基于HPC-4的亲和层析分离蛋白质。 在优选的实施方案中，特异性蛋白酶切割位点插入在表位和蛋白质之间，使得表位可以容易地从分离的蛋白质中去除。 在一个实例中，从插入到原核表达系统中的载体表达包括通过HPC-4与钙结合识别的十二个氨基酸表位和因子Xa切割位点的功能活性可溶性组织因子。 使用固定在合适底物上的HPC-4单克隆抗体，可以在单个色谱步骤中快速分离重组组织因子。 一旦分离，通过用因子Xa切割除去蛋白C表位，留下功能活性的可溶性组织因子。
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公开(公告)号：US07247453B1
公开(公告)日：2007-07-24
申请号：US08259321
申请日：1994-06-10
申请人： Alireza Rezaie , Charles T. Esmon
发明人： Alireza Rezaie , Charles T. Esmon
IPC分类号： C12P21/08 , A61K39/395 , C07K16/18
CPC分类号： C07K16/40 , C07K2317/55
摘要： A Ca2+ dependent recombinant antibody that specifically binds to a specific twelve peptide sequence (E D Q V D P R L I D G K) in the activation region of the Protein C has been constructed. The antibody does not bind to Activated Protein C and can be used to inhibit activation of Protein C by thrombin-thrombomodulin, in purification of Protein C, and in treatment of tumors.
摘要翻译： 已经构建了在蛋白C的活化区域中特异性结合特异性十二肽序列（E D Q V D P R L I D G K）的Ca 2+ 2 + >重组抗体。 抗体不结合活化蛋白C，可用于通过凝血酶 - 血栓调节蛋白，蛋白C的纯化和肿瘤的治疗来抑制蛋白C的活化。
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3.发明申请METHODS AND COMPOSITIONS FOR ACTIVATED PROTEIN C WITH REDUCED ANTI-COAGULANT PROPERTIES 有权活性蛋白C与降低抗凝血性质的方法和组合物公开(公告)号：US20080058265A1
公开(公告)日：2008-03-06
申请号：US11848080
申请日：2007-08-30
申请人： Alireza Rezaie , Likui Yang
发明人： Alireza Rezaie , Likui Yang
CPC分类号： C12N9/6464 , A61K38/00 , C12Y304/21069
摘要： This invention relates to a novel form of protein C or activated protein C. More specifically, the invention is directed to a variant of protein C that is activated at a higher rate than wild-type or other variants and produces an activated protein C with reduced anticoagulant properties while retaining the protective anti-inflammatory and anti-apoptotic properties of wild-type activated protein C. This novel APC variant will be beneficial for treating inflammatory and apoptotic disorders with a reduced risk for bleeding.
摘要翻译： 本发明涉及蛋白C或活化蛋白C的新形式。更具体地，本发明涉及以比野生型或其他变体更高的速率活化的蛋白C的变体，并产生具有降低的活化蛋白C 同时保留野生型活化蛋白C的保护性抗炎和抗凋亡特性。这种新的APC变体将有益于治疗具有降低的出血风险的炎症和凋亡障碍。
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公开(公告)号：US06794493B2
公开(公告)日：2004-09-21
申请号：US09828592
申请日：2001-04-06
申请人： Timothy Walston , Scott Cooper , Alireza Rezaie
发明人： Timothy Walston , Scott Cooper , Alireza Rezaie
IPC分类号： A61K3514
CPC分类号： C07K14/8128 , A61K38/57
摘要： Mutant antithrombin modified to carry a more positive charge in the H-helix is provided. The modified antithrombin modulates the activities of thrombin, and extends the bioavailability of factor VIII.
摘要翻译： 提供修饰为在H-螺旋中携带更多正电荷的突变抗凝血酶。 改性抗凝血酶调节凝血酶的活性，延长因子VIII的生物利用度。
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