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    Human neuronal nicotinic acetylcholine receptor compositions and methods employing same
    4.
    发明授权
    Human neuronal nicotinic acetylcholine receptor compositions and methods employing same 失效
    人神经元烟碱乙酰胆碱受体组合物及其应用方法

    公开(公告)号：US07119067B2

    公开(公告)日：2006-10-10

    申请号：US10349836

    申请日：2003-01-23

    申请人： Kathryn J. Elliott ,  Michael M. Harpold

    发明人： Kathryn J. Elliott ,  Michael M. Harpold

    IPC分类号： C07K14/705

    CPC分类号： G01N33/5008 ,  C07K14/70571 ,  G01N33/566

    摘要： Nucleic acid molecules encoding human neuronal nicotinic acetylcholine receptor alpha and beta subunits, mammalian and amphibian cells containing the nucleic acid molecules, and methods for producing alpha and beta subunits are provided. In particular, nucleic acid molecules encoding α6 subunits and molecules encoding β3 subunits of human neuronal nicotinic acetylcholine receptors are provided. In addition, combinations of a plurality of subunits, such as one or more of α1, α2, α3, α4, α5, α6 and/or α7 subunits in combination with one or more of β3 subunits or such as one or more of β2, β3 and/or β4 subunits in combination with an α6 subunit are provided.

    摘要翻译： 提供编码人神经元烟碱乙酰胆碱受体α和β亚基的核酸分子，含有核酸分子的哺乳动物和两栖动物细胞，以及产生α和β亚基的方法。 特别地，提供了编码α6个亚基的核酸分子和编码人神经元烟碱乙酰胆碱受体的β3 N亚基的分子。 此外，多个亚基的组合，例如α1，α2，α3，α3，α3， 4个，α5个，α6个和/或α7个亚基，与β3的一个或多个组合 β亚基或诸如β2，β3和/或β4亚单位中的一个或多个的α亚基， 提供了SUB> 6 亚基。

    Human neuronal nicotinic acetylcholine receptor & subunit methods employing same
    5.
    发明授权
    Human neuronal nicotinic acetylcholine receptor & subunit methods employing same 失效
    人类神经元烟碱乙酰胆碱受体和亚基方法

    公开(公告)号：US06664375B2

    公开(公告)日：2003-12-16

    申请号：US09892985

    申请日：2001-06-27

    申请人： Kathryn J. Elliott ,  Steven B. Ellis ,  Michael M. Harpold

    发明人： Kathryn J. Elliott ,  Steven B. Ellis ,  Michael M. Harpold

    IPC分类号： C07K14705

    CPC分类号： C07K14/70571

    摘要： DNA encoding human neuronal nicotinic acetylcholine receptor alpha and beta subunits, mammalian and amphibian cells containing the DNA, methods for producing &agr; and &bgr; subunits and isolated or substantially pure &agr;4, &agr;7 and &bgr;4 subunits are provided. In addition, cells that expresses these subunits singly or combination with other subunits of nicotinic acetylcholine receptors and methods using the cells are provided.

    摘要翻译： 提供了编码人神经元烟碱乙酰胆碱受体α和β亚基的DNA，含有DNA的哺乳动物和两栖动物细胞，产生α和β亚基的方法以及分离或基本上纯的α4，α7和β4亚单位。 此外，提供单独表达这些亚单位或与烟碱乙酰胆碱受体的其它亚基组合的细胞和使用细胞的方法。

    Human neuronal nicotinic acetylcholine receptor &agr;6 and &bgr;3 nucleic acid
    6.
    发明授权
    Human neuronal nicotinic acetylcholine receptor &agr;6 and &bgr;3 nucleic acid 失效
    人神经元烟碱乙酰胆碱受体α6和β3核酸

    公开(公告)号：US06485967B1

    公开(公告)日：2002-11-26

    申请号：US08484722

    申请日：1995-06-07

    申请人： Kathryn J. Elliott ,  Michael M. Harpold

    发明人： Kathryn J. Elliott ,  Michael M. Harpold

    IPC分类号： C12N510

    CPC分类号： G01N33/5008 ,  C07K14/70571 ,  G01N33/566

    摘要： DNA encoding human neuronal nicotinic acetylcholine receptor alpha and beta subunits, mammalian and amphibian cells containing said DNA, methods for producing alpha and beta subunits and recombinant (i.e., isolated or substantially pure) alpha subunits (specifically &agr;6) and beta subunits (specifically &bgr;3) are provided. In addition, combinations of a plurality of subunits (i.e., one or more of as &agr;1, &agr;2, &agr;3, &agr;4, &agr;5, &agr;6 and/or &agr;7 subunits in combination with one or more of &bgr;3 subunits are provided.

    摘要翻译： 编码人类神经元烟碱乙酰胆碱受体α和β亚基的DNA，含有所述DNA的哺乳动物和两栖动物细胞，用于产生α和β亚基和重组（即，分离或基本上纯的）α亚单位（特别是α6）和β亚单位（特别是β3） 被提供。 此外，提供了多个亚基（即，α1，α2，α3，α4，α5，α6和/或α7亚基中的一个或多个与β3亚基中的一个或多个组合的组合）。

    Human neuronal nicotinic acetylcholine receptor compositions and methods employing same
    7.
    发明授权
    Human neuronal nicotinic acetylcholine receptor compositions and methods employing same 失效

    公开(公告)号：US06524789B1

    公开(公告)日：2003-02-25

    申请号：US08660451

    申请日：1996-06-07

    申请人： Kathryn J. Elliott ,  Michael M. Harpold

    发明人： Kathryn J. Elliott ,  Michael M. Harpold

    IPC分类号： C12Q168

    CPC分类号： G01N33/5008 ,  C07K14/70571 ,  G01N33/566

    摘要： Nucleic acid molecules encoding human neuronal nicotinic acetylcholine receptor alpha and beta subunits, mammalian and amphibian cells containing the nucleic acid molecules, and methods for producing alpha and beta subunits are provided. In particular, nucleic acid molecules encoding &agr;6 subunits and molecules encoding &bgr;3 subunits of human neuronal nicotinic acetylcholine receptors are provided. In addition, combinations of a plurality of subunits, such as one or more of &agr;1, &agr;2, &agr;3, &agr;4, &agr;5, &agr;6 and/or &agr;7 subunits in combination with one or more of &bgr;3 subunits or such as one or more of &bgr;2, &bgr;3 and/or &bgr;4 subunits in combination with an &agr;6 subunit are provided.

    Human neuronal nicotinic acetylcholine receptor compositions and methods employing same
    8.
    发明授权
    Human neuronal nicotinic acetylcholine receptor compositions and methods employing same 失效
    人神经元烟碱乙酰胆碱受体组合物及其应用方法

    公开(公告)号：US06303753B1

    公开(公告)日：2001-10-16

    申请号：US09217345

    申请日：1998-12-21

    申请人： Kathryn J. Elliott ,  Steven B. Ellis ,  Michael M. Harpold

    发明人： Kathryn J. Elliott ,  Steven B. Ellis ,  Michael M. Harpold

    IPC分类号： C07K100

    CPC分类号： C07K14/70571

    摘要： DNA encoding human neuronal nicotinic acetylcholine receptor alpha and beta subunits, mammalian and amphibian cells containing the DNA, methods for producing &agr; and &bgr; subunits and isolated or substantially pure &agr;4, &agr;7 and &bgr;4 subunits are provided. In addition, cells that expresses these subunits singly or combination with other subunits of nicotinic acetylcholine receptors and methods using the cells are provided.

    摘要翻译： 提供了编码人神经元烟碱乙酰胆碱受体α和β亚基的DNA，含有DNA的哺乳动物和两栖动物细胞，产生α和β亚基的方法以及分离或基本上纯的α4，α7和β4亚单位。 此外，提供单独表达这些亚单位或与烟碱乙酰胆碱受体的其它亚基组合的细胞和使用细胞的方法。

    Invertase genes and uses thereof
    10.
    发明授权
    Invertase genes and uses thereof 失效
    转化酶基因及其用途

    公开(公告)号：US5665579A

    公开(公告)日：1997-09-09

    申请号：US245809

    申请日：1994-05-17

    申请人： Leona C. Fitzmaurice ,  T. Erik Mirkov ,  Kathryn J. Elliott ,  William Owen Butler ,  Yoshihiro Konno ,  Craig Duane Dickinson

    发明人： Leona C. Fitzmaurice ,  T. Erik Mirkov ,  Kathryn J. Elliott ,  William Owen Butler ,  Yoshihiro Konno ,  Craig Duane Dickinson

    IPC分类号： A01H5/00 ,  C12N5/10 ,  C12N9/24 ,  C12N9/26 ,  C12N15/09 ,  C12N15/113 ,  C12N15/82 ,  C12N15/29 ,  C12N15/56

    CPC分类号： C12N15/1137 ,  C12N15/8245 ,  C12N9/2408 ,  C12N9/2431 ,  C12Y302/01026 ,  C07K2319/02

    摘要： Transgenic plants that are modified to produce fruits that have altered levels of soluble solids compared to non-transgenic species of the same species are provided. The transgenic plants are modified by introduction of DNA constructs that encode invertase operatively linked to DNA encoding regulatory regions that direct transcription of the DNA encoding invertase and to DNA encoding sequences that direct proper processing of the invertase through the secretory pathways of the plant and targeting of the invertase to the vacuole.In particular, DNA constructs encoding tomato plant vacuolar invertase in operative linkage with a developmentally regulated promoter region are provided. Preferred regulatory and structural DNA is obtained from genomic DNA clones and cDNA clones encoding tomato fruit vacuolar invertases from the commercial tomato plant, Lycopersicon esculentum, and wild tomato plant, Lycopersicon pimpinellifolium.Probes derived from the genomic DNA and cDNA, antibodies specific for tomato fruit invertase, and uses therefore, are also provided.

    摘要翻译： 提供了与相同物种的非转基因物种相比，被修饰以产生具有改变的可溶性固形物水平的水果的转基因植物。 通过引入编码转化酶的DNA构建体来修饰转基因植物，所述DNA构建体可操作地连接到编码调节区的DNA，所述编码调控区直接转录编码转化酶的DNA，以及DNA编码序列，其通过植物的分泌途径引导转化酶的适当加工， 转化为液泡。 特别地，提供了编码与发育调节的启动子区域操作性连锁的番茄植物液泡转化酶的DNA构建体。 优选的调节和结构DNA从基因组DNA克隆和编码来自商业番茄植物番茄番茄番茄果实空泡转化酶和野生番茄植物番茄（Pycpinellifolium）的cDNA克隆获得。 来自基因组DNA和cDNA的探针，对番茄果实转化酶特异性的抗体，以及因此也被使用。