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1.发明申请Use of hydroxyalkanoic acids as substrates for production of poly-hydroxyalkanoates by methane-oxidizing bacteria 审中-公开使用羟基链烷酸作为生产基于甲烷氧化细菌的聚羟基链烷酸酯的底物公开(公告)号：US20110159556A1
公开(公告)日：2011-06-30
申请号：US12928323
申请日：2010-12-08
IPC分类号： C12P7/62
CPC分类号： C12P7/625
摘要： A method of biosynthesis of polyhydroxyalkanoates (PHA) is provided that includes providing a type II methanotrophic bacteria, and disposing the type II methanotrophic bacteria in an unbalanced growth condition, where the unbalanced growth condition includes a nutrient-deficient media and a hydroxyalkanoic acid, and where the nutrient-deficient media has an absence of an essential nutrient required for cell replication of the type II methanotrophic bacteria.
摘要翻译： 提供了一种聚羟基链烷酸酯（PHA）的生物合成方法，其包括提供II型甲烷营养细菌，并将II型甲烷营养细菌置于不平衡生长条件下，其中不平衡生长条件包括营养缺乏的培养基和羟基链烷酸，以及 其中营养缺陷培养基不具有II型甲烷营养细菌的细胞复制所需的必需营养物质。
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公开(公告)号：US20130052681A1
公开(公告)日：2013-02-28
申请号：US13590603
申请日：2012-08-21
IPC分类号： C12Q1/04
CPC分类号： C12Q1/04
摘要： A method of selection for type II methanotrophs is provided that includes enriching a microbial feedstock using a non-sterile bioreactor with a methane source and a nitrogen source, where the microbial feedstock includes a mixture of Type I and Type II methanotrophic cells, where an inhibited growth of the Type I methanotrophic cells and an enhanced growth of the Type II methanotrophic cells forms. The method further includes exposing intermittently the enriched microbial feedstock to i) nitrate, ii) urea, or i) and ii), where enhanced growth of the Type II methanotrophs is established, and exposing the Type II methanotrophs to an unbalanced growth condition where production of polyhydroxybutyrate is induced.
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公开(公告)号：US20130071890A1
公开(公告)日：2013-03-21
申请号：US13421771
申请日：2012-03-15
申请人： Craig S. Criddle , John R. Hart , Wei-Min Wu , Eric R. Sundstrom , Margaret C. Morse , Sarah L. Billington , Katherine H. Rostkowski , Curtis W. Frank
发明人： Craig S. Criddle , John R. Hart , Wei-Min Wu , Eric R. Sundstrom , Margaret C. Morse , Sarah L. Billington , Katherine H. Rostkowski , Curtis W. Frank
IPC分类号： C12P7/62
CPC分类号： C12P7/625 , C07D319/12 , C08J11/12 , C08J2367/04 , Y02E50/343 , Y02W30/703
摘要： Methods for producing bioplastics from biogas include techniques for the production of PHB using a dirty biogas (e.g., methane from landfill, digester) as both a power source for the process and as feedstock. Biogas is split into two streams, one for energy to drive the process, another as feedstock. Advantageously, the techniques may be implemented off the power grid with no dependence upon agricultural products for feedstock.
摘要翻译： 用于从沼气生产生物塑料的方法包括使用脏生物气体（例如来自垃圾填埋场，蒸煮器的甲烷）作为工艺的动力源和作为原料生产PHB的技术。 生物气分为两股，一种是用于驱动该过程的能量，另一种是原料。 有利地，这些技术可以从电力网上实施，而不依赖于农产品用于原料。
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公开(公告)号：US09062340B2
公开(公告)日：2015-06-23
申请号：US13590603
申请日：2012-08-21
CPC分类号： C12Q1/04
摘要： A method of selection for type II methanotrophs is provided that includes enriching a microbial feedstock using a non-sterile bioreactor with a methane source and a nitrogen source, where the microbial feedstock includes a mixture of Type I and Type II methanotrophic cells, where an inhibited growth of the Type I methanotrophic cells and an enhanced growth of the Type II methanotrophic cells forms. The method further includes exposing intermittently the enriched microbial feedstock to i) nitrate, ii) urea, or i) and ii), where enhanced growth of the Type II methanotrophs is established, and exposing the Type II methanotrophs to an unbalanced growth condition where production of polyhydroxybutyrate is induced.
摘要翻译： 提供了选择II型甲烷培养物的方法，其包括使用具有甲烷源和氮源的非无菌生物反应器富集微生物原料，其中微生物原料包括I型和II型甲烷营养细胞的混合物，其中被抑制 I型甲烷营养细胞的生长和II型甲烷营养细胞增殖的形成。 该方法还包括间歇地将富集的微生物原料暴露于i）硝酸盐，ii）尿素，或i）和ii），其中建立了II型甲烷营养物的增强生长，并将II型甲烷营养物暴露于不平衡的生长条件，其中生产 的多羟基丁酸酯被诱导。
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5.发明申请High Solids Fermentation for Synthesis of Polyhydroxyalkanoates From Gas Substrates 审中-公开用于从气体底物合成聚羟基链烷酸酯的高固体发酵公开(公告)号：US20120028321A1
公开(公告)日：2012-02-02
申请号：US12901343
申请日：2010-10-08
IPC分类号： C12P7/42
CPC分类号： C12P7/625
摘要： Production of polyhydroxyalkanoates (PHAs) is performed by delivering substrates such as methane in gas phase during a high solids fermentation. Microorganisms are grown under balanced conditions, then gas phase substrates are delivered under unbalanced conditions to produce PHA granules inside the cells. The cells containing these granules are lysed and the bioplastic powder recovered. The balanced phase growth may occur in submerged liquid cultures or attached as biofilms to a surface.
摘要翻译： 聚羟基链烷酸酯（PHA）的生产通过在高固体发酵过程中以气相递送诸如甲烷的基质来进行。 微生物在平衡条件下生长，然后气相底物在不平衡条件下输送以在细胞内产生PHA颗粒。 将含有这些颗粒的细胞裂解并回收生物塑料粉末。 平衡相生长可能发生在浸没液体培养物中或作为生物膜附着到表面。
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6.发明授权High throughput system for isolation, growth, and detection of lipid inclusions in bacteria 有权用于细菌中脂质包裹体的分离，生长和检测的高通量系统公开(公告)号：US09234229B2
公开(公告)日：2016-01-12
申请号：US12928312
申请日：2010-12-07
IPC分类号： C12Q1/04
CPC分类号： C12Q1/04
摘要： A method of high throughput growth and quantitative analysis of microorganisms is provided that includes providing a microtiter plate growth and gas delivery system having well plates disposed for growth of the microorganisms, and providing a spectroscopic screening system disposed to analyze lipid inclusions of the microorganisms.
摘要翻译： 提供了一种高通量生长和微生物定量分析的方法，其包括提供微孔板生长和气体递送系统，其具有设置用于生长微生物的孔板，并提供设置用于分析微生物的脂质包涵体的光谱筛选系统。
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7.发明申请High throughput system for isolation, growth, and detection of lipid inclusions in bacteria 有权用于细菌中脂质包裹体的分离，生长和检测的高通量系统公开(公告)号：US20110160067A1
公开(公告)日：2011-06-30
申请号：US12928312
申请日：2010-12-07
IPC分类号： C40B30/00
CPC分类号： C12Q1/04
摘要： A method of high throughput growth and quantitative analysis of microorganisms is provided that includes providing a microtiter plate growth and gas delivery system having well plates disposed for growth of the microorganisms, and providing a spectroscopic screening system disposed to analyze lipid inclusions of the microorganisms.
摘要翻译： 提供了一种高通量生长和微生物定量分析的方法，其包括提供微孔板生长和气体递送系统，其具有设置用于生长微生物的孔板，并提供设置用于分析微生物的脂质包涵体的光谱筛选系统。
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