公开(公告)号：US20220089756A1

公开(公告)日：2022-03-24

申请号：US17053728

申请日：2019-05-08

Applicant: Amgen Inc.

Inventor： John K. KAWOOYA ,  Alex W. JACOBITZ ,  Christopher MOHR ,  Stephen SMITH ,  Kevin GRAHAM ,  Ray Lieh Yoon LOW ,  Oliver THIEL ,  Dante ROMANINI ,  Victoria WAGNER ,  Neeraj J. AGRAWAL

IPC: C07K16/28 ,  C07K16/46

Abstract: The present invention is directed to a CH3-containing molecule comprising (a) a first polypeptide comprising a CH3 domain and a negatively charged domain comprising consecutive negatively charged amino acid residues; and (b) a second polypeptide comprising a CH3 domain and a positively charged domain comprising consecutive positively charged amino acid residues. Also provided are methods for conjugating synthetic molecules to a multi-specific antigen binding molecule.

公开(公告)号：US20220064610A1

公开(公告)日：2022-03-03

申请号：US17299000

申请日：2019-12-09

Applicant: AMGEN INC.

Inventor： Neeraj J. AGRAWAL ,  Kristine M. DARIS ,  Jennitte L. STEVENS ,  Huong Thi Ngoc LE ,  Noelia Blanco TALAVAN

IPC: C12N9/12 ,  C12N15/85 ,  C07K14/725 ,  C12N15/90 ,  A61K35/17

Abstract: PiggyBac transposases engineered to increase stability in a cell. The engineered piggyBac transposases are useful for stably transforming cells, cell line development, genome modification, and improving titer of recombinant proteins, among other uses.