公开(公告)号：US20220089756A1

公开(公告)日：2022-03-24

申请号：US17053728

申请日：2019-05-08

Applicant: Amgen Inc.

Inventor： John K. KAWOOYA ,  Alex W. JACOBITZ ,  Christopher MOHR ,  Stephen SMITH ,  Kevin GRAHAM ,  Ray Lieh Yoon LOW ,  Oliver THIEL ,  Dante ROMANINI ,  Victoria WAGNER ,  Neeraj J. AGRAWAL

IPC: C07K16/28 ,  C07K16/46

Abstract: The present invention is directed to a CH3-containing molecule comprising (a) a first polypeptide comprising a CH3 domain and a negatively charged domain comprising consecutive negatively charged amino acid residues; and (b) a second polypeptide comprising a CH3 domain and a positively charged domain comprising consecutive positively charged amino acid residues. Also provided are methods for conjugating synthetic molecules to a multi-specific antigen binding molecule.

公开(公告)号：US20190127418A1

公开(公告)日：2019-05-02

申请号：US16093994

申请日：2017-07-21

Applicant: Amgen Inc.

Inventor： John K. KAWOOYA

IPC: C07K1/22 ,  C07K16/00 ,  C07K1/34 ,  B01D15/34 ,  B01D15/38 ,  B01D15/42

Abstract: The present invention relates to methods of purifying proteins containing Fc regions, such as antibodies and Fc fusion proteins. In particular, the present invention relates to a purification method resulting in reduced levels of aggregate protein comprising adsorbing an Fc region-containing protein to a temperature-responsive protein A resin and eluting the protein from the resin at a temperature below 35 C with an elution buffer comprising a chaotropic agent, a sugar alcohol, and at least one amino acid. Methods of separating fully assembled antibodies from half antibody forms thereof using the elution buffer are also described.