公开(公告)号：US10519475B1

公开(公告)日：2019-12-31

申请号：US16198545

申请日：2018-11-21

Applicant: Amyris, Inc.

Inventor： Jessica Walter ,  Joshua A. Lerman ,  Michael Leavell ,  Benjamin Yap

IPC: C12P19/12 ,  C12N15/81 ,  C07H13/04 ,  C12N9/10 ,  C12N9/24 ,  C12N9/12 ,  C12N9/88

Abstract: Provided herein are genetically modified yeast cells capable of producing one or more human milk oligosaccharides. The yeast cells include one or more heterologous nucleic acids that encode enzymes of a human milk oligosaccharide biosynthetic pathway. The yeast cells do not include a heterologous nucleic acid encoding a fucokinase. Also provided are fermentation compositions including the disclosed genetically modified yeast cells, and related methods of producing and recovering human milk oligosaccharides generated by the yeast cells.