公开(公告)号：US11685934B2

公开(公告)日：2023-06-27

申请号：US16646028

申请日：2018-09-12

Applicant: Amyris, Inc.

Inventor： Jessica Walter

IPC: C12N15/90 ,  C12N9/22 ,  C12N15/11 ,  C12N15/81

CPC classification number: C12N15/905 ,  C12N9/22 ,  C12N15/11 ,  C12N15/815 ,  C12N2310/20 ,  C12N2800/80

Abstract: The present invention provides high efficiency targeted and marker-less single or simultaneous multiple integrations using nucleases and a stable plasmid in Kluyveromyces host cells.

公开(公告)号：US12188044B2

公开(公告)日：2025-01-07

申请号：US18199278

申请日：2023-05-18

Applicant: Amyris, Inc.

Inventor： Jessica Walter

IPC: C12N15/90 ,  C12N9/22 ,  C12N15/11 ,  C12N15/81

Abstract: The present invention provides high efficiency targeted and marker-less single or simultaneous multiple integrations using nucleases and a stable plasmid in Kluyveromyces host cells.

公开(公告)号：US10519475B1

公开(公告)日：2019-12-31

申请号：US16198545

申请日：2018-11-21

Applicant: Amyris, Inc.

Inventor： Jessica Walter ,  Joshua A. Lerman ,  Michael Leavell ,  Benjamin Yap

IPC: C12P19/12 ,  C12N15/81 ,  C07H13/04 ,  C12N9/10 ,  C12N9/24 ,  C12N9/12 ,  C12N9/88

Abstract: Provided herein are genetically modified yeast cells capable of producing one or more human milk oligosaccharides. The yeast cells include one or more heterologous nucleic acids that encode enzymes of a human milk oligosaccharide biosynthetic pathway. The yeast cells do not include a heterologous nucleic acid encoding a fucokinase. Also provided are fermentation compositions including the disclosed genetically modified yeast cells, and related methods of producing and recovering human milk oligosaccharides generated by the yeast cells.