公开(公告)号：US20240060056A1

公开(公告)日：2024-02-22

申请号：US18267928

申请日：2021-12-16

Applicant: Amyris, Inc.

Inventor： Jessica WALTER ,  Wenzong LI ,  Christopher F. MUGLER ,  Victoria HSIAO

IPC: C12N9/10 ,  C12P19/18 ,  C12N1/16

CPC classification number: C12N9/1051 ,  C12P19/18 ,  C12N1/165 ,  C12Y204/01146 ,  C12R2001/85

Abstract: Provided herein are variant β-1,3-N-acetylglucosaminyltransferase polypeptides capable of producing lacto-n-neotetraose, yeast cells capable of producing one or more human milk oligosaccharides, and methods of making such cells. Also, provided are fermentation compositions including the disclosed genetically modified yeast cells, and related methods of producing and recovering HMOs generated by the yeast cells.

公开(公告)号：US20200263205A1

公开(公告)日：2020-08-20

申请号：US16646028

申请日：2018-09-12

Applicant: AMYRIS, INC.

Inventor： Jessica WALTER

IPC: C12N15/90 ,  C12N15/81 ,  C12N15/11 ,  C12N9/22

Abstract: The present invention provides high efficiency targeted and marker-less single or simultaneous multiple integrations using nucleases and a stable plasmid in Kluyveromyces host cells.

公开(公告)号：US20240182927A1

公开(公告)日：2024-06-06

申请号：US18199278

申请日：2023-05-18

Applicant: Amyris, Inc.

Inventor： Jessica WALTER

IPC: C12N15/90 ,  C12N9/22 ,  C12N15/11 ,  C12N15/81

CPC classification number: C12N15/905 ,  C12N9/22 ,  C12N15/11 ,  C12N15/815 ,  C12N2310/20 ,  C12N2800/80

Abstract: The present invention provides high efficiency targeted and marker-less single or simultaneous multiple integrations using nucleases and a stable plasmid in Kluyveromyces host cells.

公开(公告)号：US20220298536A1

公开(公告)日：2022-09-22

申请号：US17634392

申请日：2020-08-13

Applicant: Amyris, Inc.

Inventor： Jessica WALTER ,  Dominic PINEL

IPC: C12P19/04

Abstract: Provided herein are genetically modified yeast cells capable of producing one or more human milk oligosaccharides (HMOs) and methods of making such cells. The yeast cells are engineered to comprise a heterologous nucleic acid encoding a transporter protein and one or more heterologous nucleic acids that encode enzymes of a HMO biosynthetic pathway. Also provided are fermentation compositions including the disclosed genetically modified yeast cells, and related methods of producing and recovering HMOs generated by the yeast cells.