公开(公告)号：US20240384315A1

公开(公告)日：2024-11-21

申请号：US18688617

申请日：2022-09-02

Applicant: Amyris, Inc.

Inventor： Dominic PINEL ,  Victoria HSIAO ,  Diana Bahraini MARINA ,  Derek A. ABBOTT

IPC: C12P19/00 ,  A23L33/00 ,  C12N9/04 ,  C12N9/10 ,  C12N9/12 ,  C12N9/22 ,  C12N9/88 ,  C12N15/11 ,  C12N15/113 ,  C12N15/81

Abstract: Provided herein are host cells capable of producing a human milk oligosaccharide (HMO), such as yeast cells that are deficient in expression or activity of an endogenous oxidoreductase. Also provided are fermentation compositions including the disclosed host cells, as well as related methods of producing and recovering HMOs generated by the host cells.

公开(公告)号：US20220298536A1

公开(公告)日：2022-09-22

申请号：US17634392

申请日：2020-08-13

Applicant: Amyris, Inc.

Inventor： Jessica WALTER ,  Dominic PINEL

IPC: C12P19/04

Abstract: Provided herein are genetically modified yeast cells capable of producing one or more human milk oligosaccharides (HMOs) and methods of making such cells. The yeast cells are engineered to comprise a heterologous nucleic acid encoding a transporter protein and one or more heterologous nucleic acids that encode enzymes of a HMO biosynthetic pathway. Also provided are fermentation compositions including the disclosed genetically modified yeast cells, and related methods of producing and recovering HMOs generated by the yeast cells.