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    Genes encoding proteins with pesticidal activity
    1.
    发明申请
    Genes encoding proteins with pesticidal activity 有权
    编码具有杀虫活性的蛋白质的基因

    公开(公告)号：US20060021096A1

    公开(公告)日：2006-01-26

    申请号：US11224624

    申请日：2005-09-12

    申请人： Andre Abad ,  Hua Dong ,  Rafael Herrmann ,  Albert Lu ,  Billy McCutchen ,  Janet Rice ,  Eric Schepers ,  James Wong

    发明人： Andre Abad ,  Hua Dong ,  Rafael Herrmann ,  Albert Lu ,  Billy McCutchen ,  Janet Rice ,  Eric Schepers ,  James Wong

    IPC分类号： A01H5/00 ,  A01H1/00 ,  C07K14/415

    CPC分类号： C07K14/325 ,  C12N15/8286 ,  Y02A40/162

    摘要： Compositions and methods for protecting a plant from an insect pest are provided. The invention provides mutagenized nucleic acids that have been engineered to encode pesticidal polypeptides having increased resistance to proteolytic degradation by a plant protease. In particular, nucleic acid sequences encoding pesticidal polypeptides modified to comprise a proteolytic protection site that confers resistance to degradation or proteolytic inactivation by a plant protease are provided. Particular embodiments of the invention provide pesticidal polypeptide compositions and formulations, expression cassettes, and transformed plants, plant cells, and seeds. These compositions find use in methods for controlling pests, especially plant pests. Novel plant proteases, sequences encoding these proteases, and methods for their use are also provided.

    摘要翻译： 提供了保护植物免受害虫害虫的组合物和方法。 本发明提供已被工程化以编码具有增加的抗植物蛋白酶对蛋白水解降解的杀虫多肽的诱变的核酸。 特别地，提供了编码杀虫多肽的核酸序列，其被修饰为包含赋予植物蛋白酶降解或蛋白水解灭活抗性的蛋白水解保护位点。 本发明的具体实施方案提供杀虫多肽组合物和制剂，表达盒和转化植物，植物细胞和种子。 这些组合物可用于防治害虫，特别是植物害虫的方法。 还提供了新型植物蛋白酶，编码这些蛋白酶的序列及其使用方法。

    Plant activation of insect toxin
    2.
    发明申请
    Plant activation of insect toxin 有权
    植物激活昆虫毒素

    公开(公告)号：US20050166284A1

    公开(公告)日：2005-07-28

    申请号：US11021115

    申请日：2004-12-22

    申请人： Andre Abad ,  Ronald Flannagan ,  Rafael Herrmann ,  Albert Lu ,  Billy McCutchen ,  James Presnail ,  Janet Rice ,  James Wong ,  Cao-Guo Yu

    发明人： Andre Abad ,  Ronald Flannagan ,  Rafael Herrmann ,  Albert Lu ,  Billy McCutchen ,  James Presnail ,  Janet Rice ,  James Wong ,  Cao-Guo Yu

    IPC分类号： A01H1/00 ,  A01H5/00 ,  C07K14/325 ,  C12N9/64 ,  C12N15/82

    CPC分类号： C12N15/8286 ,  C12N9/641 ,  Y02A40/162

    摘要： Compositions and methods for protecting a plant from an insect pest are provided. In particular, nucleic acid sequences encoding insect protoxins modified to comprise at least one proteolytic activation site that is sensitive to a plant protease or an insect gut protease are provided. Cleavage of the modified protoxin at the proteolytic activation site by a protease produces an active insect toxin. Methods of using the modified insect protoxin nucleic acid sequences and the polypeptides they encode to protect a plant from an insect pest are provided. Particular embodiments of the invention further provide modified insect protoxin compositions and formulations, expression cassettes, and transformed plants, plant cells, and seeds.

    摘要翻译： 提供了保护植物免受害虫害虫的组合物和方法。 特别地，提供了编码昆虫原毒素的核酸序列，其被修饰为包含对植物蛋白酶或昆虫肠道蛋白酶敏感的至少一个蛋白水解活化位点。 通过蛋白酶在蛋白水解活化位点切割修饰的原毒素会产生活性昆虫毒素。 提供了使用修饰的昆虫原毒素核酸序列及其编码的多肽保护植物免受害虫的方法。 本发明的具体实施方案还提供了改良的昆虫原毒素组合物和制剂，表达盒，以及转化的植物，植物细胞和种子。

    Genes encoding proteins with pesticidal activity
    3.
    发明授权
    Genes encoding proteins with pesticidal activity 有权
    编码具有杀虫活性的蛋白质的基因

    公开(公告)号：US07378499B2

    公开(公告)日：2008-05-27

    申请号：US11224624

    申请日：2005-09-12

    申请人： André Abad ,  Hua Dong ,  Rafael Herrmann ,  Albert Lu ,  Billy F. McCutchen ,  Janet Rice ,  Eric Schepers ,  James Wong

    发明人： André Abad ,  Hua Dong ,  Rafael Herrmann ,  Albert Lu ,  Billy F. McCutchen ,  Janet Rice ,  Eric Schepers ,  James Wong

    IPC分类号： C07K1/00

    CPC分类号： C07K14/325 ,  C12N15/8286 ,  Y02A40/162

    摘要： Compositions and methods for protecting a plant from an insect pest are provided. The invention provides mutagenized nucleic acids that have been engineered to encode pesticidal polypeptides having increased resistance to proteolytic degradation by a plant protease. In particular, nucleic acid sequences encoding pesticidal polypeptides modified to comprise a proteolytic protection site that confers resistance to degradation or proteolytic inactivation by a plant protease are provided. Particular embodiments of the invention provide pesticidal polypeptide compositions and formulations, expression cassettes, and transformed plants, plant cells, and seeds. These compositions find use in methods for controlling pests, especially plant pests. Novel plant proteases, sequences encoding these proteases, and methods for their use are also provided.

    摘要翻译： 提供了保护植物免受害虫害虫的组合物和方法。 本发明提供已被工程化以编码具有增加的抗植物蛋白酶对蛋白水解降解的杀虫多肽的诱变的核酸。 特别地，提供了编码杀虫多肽的核酸序列，其被修饰为包含赋予植物蛋白酶降解或蛋白水解灭活抗性的蛋白水解保护位点。 本发明的具体实施方案提供杀虫多肽组合物和制剂，表达盒和转化植物，植物细胞和种子。 这些组合物可用于防治害虫，特别是植物害虫的方法。 还提供了新型植物蛋白酶，编码这些蛋白酶的序列及其使用方法。

    Insect-specific protease recognition sequences
    4.
    发明申请
    Insect-specific protease recognition sequences 有权
    昆虫特异性蛋白酶识别序列

    公开(公告)号：US20060288448A1

    公开(公告)日：2006-12-21

    申请号：US11448266

    申请日：2006-06-07

    申请人： Andre Abad ,  Gary Chun ,  Ronald Flannagan ,  Rafael Herrmann ,  Albert Lu ,  Janet Rice ,  Eric Schepers

    发明人： Andre Abad ,  Gary Chun ,  Ronald Flannagan ,  Rafael Herrmann ,  Albert Lu ,  Janet Rice ,  Eric Schepers

    IPC分类号： A01H1/00 ,  C07H21/04 ,  C12N15/82 ,  C12N5/04

    CPC分类号： C12N15/8286 ,  A01N63/02 ,  C07K14/325 ,  C12N9/6472 ,  Y02A40/162

    摘要： Compositions and methods for protecting a plant from an insect pest are provided. Nucleic acid molecules encoding insect protoxins or insect toxins modified to comprise at least one proteolytic activation site that is sensitive to an insect gut protease are provided. Cleavage of a modified insect protoxin at the proteolytic activation site by an insect gut protease produces an active insect toxin in the gut of the insect pest. Cleavage of a modified insect toxin of the invention at a proteolytic activation site results in the production of an active insect toxin in the insect gut that displays improved pesticidal activity relative to the insect toxin that lacks the proteolytic activation site. Methods of using the modified insect protoxin and modified insect toxin nucleic acid sequences and the polypeptides they encode to protect a plant from an insect pest are provided. Particular embodiments of the invention further provide modified insect protoxin and modified insect toxin compositions and formulations, expression cassettes, and transformed plants, plant cells, and seeds. Insect gut proteases and the nucleic acid molecules that encode them are also disclosed herein.

    摘要翻译： 提供了保护植物免受害虫害虫的组合物和方法。 提供编码昆虫原毒素或昆虫毒素的核酸分子，其被修饰为包含至少一个对昆虫肠道蛋白酶敏感的蛋白水解活化位点。 通过昆虫肠蛋白酶在蛋白水解活化位点切割修饰的昆虫原毒素在害虫的肠道中产生活性昆虫毒素。 在蛋白水解活化位点切割本发明的修饰的昆虫毒素导致在昆虫肠中产生活性昆虫毒素，其相对于缺乏蛋白水解活化位点的昆虫毒素显示出改善的杀虫活性。 提供了使用修饰的昆虫原毒素和修饰的昆虫毒素核酸序列及其编码的多肽以保护植物免受害虫的方法。 本发明的具体实施方案还提供改性的昆虫原毒素和修饰的昆虫毒素组合物和制剂，表达盒，以及转化的植物，植物细胞和种子。 昆虫肠道蛋白酶和编码它们的核酸分子也在本文中公开。

    Antifungal polypeptides
    6.
    发明申请
    Antifungal polypeptides 有权
    抗真菌多肽

    公开(公告)号：US20060031962A1

    公开(公告)日：2006-02-09

    申请号：US11174413

    申请日：2005-07-01

    申请人： Daniel Altier ,  Glen Dahlbacka ,  I.A. Ellanskaya ,  Rafael Herrmann ,  Jennie Hunter-Cevera ,  Billy McCutchen ,  James Presnail ,  Janet Rice ,  Eric Schepers ,  Carl Simmons ,  Tamas Torok ,  Nasser Yalpani

    发明人： Daniel Altier ,  Glen Dahlbacka ,  I.A. Ellanskaya ,  Rafael Herrmann ,  Jennie Hunter-Cevera ,  Billy McCutchen ,  James Presnail ,  Janet Rice ,  Eric Schepers ,  Carl Simmons ,  Tamas Torok ,  Nasser Yalpani

    IPC分类号： A61K38/17 ,  A01H1/00 ,  C07H21/04 ,  C12N5/04 ,  C07K14/415 ,  C12N15/82

    CPC分类号： C07K14/37 ,  A01N63/02 ,  A01N63/04 ,  A61K38/00 ,  C07K14/385 ,  C12N15/8282

    摘要： Compositions and methods for protecting a plant from a pathogen, particularly a fungal pathogen, are provided. Compositions include novel amino acid sequences, and variants and fragments thereof, for antipathogenic polypeptides that were isolated from microbial fermentation broths. Nucleic acid molecules comprising nucleotide sequences that encode the antipathogenic polypeptides of the invention are also provided. A method for inducing pathogen resistance in a plant using the nucleotide sequences disclosed herein is further provided. The method comprises introducing into a plant an expression cassette comprising a promoter operably linked to a nucleotide sequence that encodes an antipathogenic polypeptide of the invention. Compositions comprising an antipathogenic polypeptide or a transformed microorganism comprising a nucleic acid of the invention in combination with a carrier and methods of using these compositions to protect a plant from a pathogen are further provided. Transformed plants, plant cells, seeds, and microorganisms comprising a nucleotide sequence that encodes an antipathogenic polypeptide of the invention, or variant or fragment thereof, are also disclosed.

    摘要翻译： 提供了用于保护植物免受病原体，特别是真菌病原体的组合物和方法。 组合物包括从微生物发酵液中分离的抗病原体多肽的新型氨基酸序列及其变体和片段。 还提供了包含编码本发明的抗病原多肽的核苷酸序列的核酸分子。 还提供使用本文公开的核苷酸序列在植物中诱导病原体抗性的方法。 该方法包括向植物中引入包含可操作地连接到编码本发明的抗病原体多肽的核苷酸序列的启动子的表达盒。 还提供包含抗病原体多肽或包含本发明的核酸与载体组合的转化微生物的组合物，以及使用这些组合物保护植物免受病原体的方法。 还公开了包含编码本发明的抗病原体多肽的核苷酸序列或其变体或片段的转化植物，植物细胞，种子和微生物。