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公开(公告)号：US07645903B2
公开(公告)日：2010-01-12
申请号：US10531783
申请日：2003-09-12
IPC分类号： C07C275/00 , C07D213/81 , A61P37/00 , C07D233/34 , A61K31/17
CPC分类号： C07C275/30 , C07C275/28 , C07C275/32 , C07D207/16 , C08G71/02 , G01N33/6854
摘要： The present invention relates to an IgG-binding compound, which more specifically has affinity for human IgGs of κ-type and functional derivatives thereof. More specifically, the compound according to the invention comprises an N,N-alkylated urea moiety located between an aromatic part and another part, which is a linear or cyclic substituted or unsubstituted aliphatic group. The compound binds to a pocket-shaped binding site present on all human IgG κ-Fabs, which site is located between the two domains (CH1 and CL) of its constant part. Accordingly, the compound according to the invention is a ligand for human IgGs of κ-type, and consequently, the invention also relates to a separation matrix for affinity chromatography, which matrix comprises said compound, as well as to other uses of the compound.
摘要翻译： 本发明涉及IgG结合化合物，其更具体地对κ类型的人IgG及其功能性衍生物具有亲和力。 更具体地，根据本发明的化合物包含位于芳族部分和另一部分之间的N，N-烷基化脲部分，其是线性或环状取代或未取代的脂族基团。 该化合物结合存在于所有人类IgG卡帕Fabs上的口袋状结合位点，该位点位于其恒定部分的两个结构域（CH1和CL）之间。 因此，根据本发明的化合物是κ型人IgG的配体，因此，本发明还涉及用于亲和层析的分离基质，该基质包含所述化合物，以及该化合物的其它用途。
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公开(公告)号：US20060199950A1
公开(公告)日：2006-09-07
申请号：US10549083
申请日：2004-04-01
申请人： Lars Andersson , Tesfai Gebru , Tania Ahmad , John Clachan , Andreas Axén
发明人： Lars Andersson , Tesfai Gebru , Tania Ahmad , John Clachan , Andreas Axén
CPC分类号： B01D15/3828 , B01J20/286 , B01J45/00 , C07C231/02
摘要： The present invention relates to the preparation of a metal chelating separation medium, which comprises providing a compound defined by formula: R—O—CO—(CH2)n—CHNH2—CO—OH wherein R is a selectively cleavable group; and n is an integer of 1-4; reacting it with ethyl alcohol; derivatisation; cleavage of R; immobilisation of the resulting compound to a base matrix; and deprotection of the carboxylic acid. In one embodiment, the compound provided in the first step is ethyl ester of aspartic acid or of glutamic acid, and the derivatisation agent is bromo-acetic acid ethyl acetate.
摘要翻译： 本发明涉及金属螯合分离介质的制备，其包括提供由下式定义的化合物：RO-CO-（CH 2）n - > 2 -CO-OH其中R是选择性可裂解基团; n为1-4的整数; 与乙醇反应; 衍生化 R的裂解; 将所得化合物固定至基础基质; 和羧酸的脱保护。 在一个实施方案中，第一步中提供的化合物是天冬氨酸或谷氨酸的乙酯，衍生化剂是溴乙酸乙酸乙酯。
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公开(公告)号：US07175767B2
公开(公告)日：2007-02-13
申请号：US10549083
申请日：2004-04-01
申请人： Lars C. Andersson , Tesfai Gebru , Tania Ahmad , John Clachan , Andreas Axén
发明人： Lars C. Andersson , Tesfai Gebru , Tania Ahmad , John Clachan , Andreas Axén
CPC分类号： B01D15/3828 , B01J20/286 , B01J45/00 , C07C231/02
摘要： The present invention relates to the preparation of a metal chelating separation medium, which comprises providing a compound defined by formula: R—O—CO—(CH2)n—CHNH2—CO—OH wherein R is a selectively cleavable group; and n is an integer of 1–4; reacting it with ethyl alcohol; derivatisation; cleavage of R; immobilisation of the resulting compound to a base matrix; and deprotection of the carboxylic acid. In one embodiment, the compound provided in the first step is ethyl ester of aspartic acid or of glutamic acid, and the derivatisation agent is bromo-acetic acid ethyl acetate.
摘要翻译： 本发明涉及金属螯合分离介质的制备，其包括提供由下式定义的化合物：RO-CO-（CH 2）n - > 2 -CO-OH其中R是选择性可裂解基团; n为1-4的整数; 与乙醇反应; 衍生化 R的裂解; 将所得化合物固定至基础基质; 和羧酸的脱保护。 在一个实施方案中，第一步中提供的化合物是天冬氨酸或谷氨酸的乙酯，衍生化剂是溴乙酸乙酸乙酯。
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公开(公告)号：US07005071B2
公开(公告)日：2006-02-28
申请号：US10699058
申请日：2003-10-31
IPC分类号： B01D15/08
CPC分类号： B01J45/00 , B01D15/3828 , B01J20/286 , B01J20/3251 , B01J20/3265 , C07C323/58 , C07D333/36
摘要： The present invention relates to a method of generating at least one polydentate metal chelating affinity ligand, which method comprises the steps of a) providing at least one cyclic scaffold comprising a carbonyl, an adjacent sulphur and a nucleophile; b) providing at least one polydentate metal chelating affinity ligand arm, optionally in a form wherein the metal chelating functionalities are protected, on each scaffold by derivatisation of the nucleophile of said scaffolds, while retaining the cyclic structure of the scaffold; (c) ring-opening at the bond between the carbonyl and the sulphur of the derivatized scaffold by adding a reagent that adds one or more metal chelating affinity ligand arms to the scaffold; and, if required, (d) deprotecting the functionalities of the ligand arm(s) provided in step (b). In the most preferred embodiment of the method, steps (c) and (d) are performed simultaneously as one single step.
摘要翻译： 本发明涉及产生至少一种多齿金属螯合亲和配体的方法，该方法包括该步骤
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