公开(公告)号：US20050239197A1

公开(公告)日：2005-10-27

申请号：US10503266

申请日：2003-01-24

申请人： Andreas Katerkamp ,  Uwe Brinkmann ,  Frank Grawe ,  Goran Key ,  Sabine Schreiber ,  Jochen Uckelmann

发明人： Andreas Katerkamp ,  Uwe Brinkmann ,  Frank Grawe ,  Goran Key ,  Sabine Schreiber ,  Jochen Uckelmann

IPC分类号： G01N21/76 ,  B01L3/00 ,  C12M1/34 ,  C12M3/00 ,  C12Q1/02 ,  G01N21/03 ,  G01N21/64 ,  G01N21/77 ,  G01N21/85 ,  C12M1/00

CPC分类号： G01N21/6452 ,  B01L3/50853 ,  B01L2300/046 ,  B01L2300/0654 ,  B01L2300/0829 ,  B01L2300/163 ,  C12M23/12 ,  C12M23/20 ,  C12M23/38 ,  C12M41/26 ,  C12M41/30 ,  G01N21/0303 ,  G01N21/6428 ,  G01N21/7703 ,  G01N21/8507

摘要： The invention relates to a lid element which is placed on cell culture vessels in which cells contained in a liquid medium are accommodated. The lid element according to the invention is intended to allow determination of metabolism activities of cells which are contained in the cell culture vessels, by means of optical measurement methods, which can be carried out with simple handling by means of laboratory personnel. Light-guiding elements are provided on the lid element which can be fitted to the cell culture vessel and, when the lid element is fitted, project into the interior of cavities in the cell vessel. At least one optically sensitive layer is formed on one end surface and/or on the outer envelope surface of the light-guiding elements, which are preferably optical waveguides in the form of rods, for detection of chemical substance concentrations which change within the cavities.

摘要翻译： 本发明涉及一种盖元件，其被放置在容纳在液体介质中的细胞的细胞培养容器中。 根据本发明的盖元件旨在通过光学测量方法来测定细胞培养容器中包含的细胞的代谢活性，其可以通过实验室人员的简单处理来进行。 导光元件设置在盖元件上，该元件可以装配到细胞培养容器中，并且当盖元件被装配时，突出到细胞容器中的空腔内部。 至少一个光敏层形成在导光元件的一个端面和/或外包络表面上，光导元件最好是以棒状形式的光波导，用于检测腔内变化的化学物质浓度。

公开(公告)号：US06440748B1

公开(公告)日：2002-08-27

申请号：US09530505

申请日：2000-07-18

申请人： Andreas Katerkamp ,  Ulrich Kunz ,  Frank Grawe ,  Göran Key

发明人： Andreas Katerkamp ,  Ulrich Kunz ,  Frank Grawe ,  Göran Key

IPC分类号： G01N2100

CPC分类号： G01N21/648 ,  G01N21/6428 ,  G01N21/7703 ,  G01N2021/6419 ,  G01N2021/6441 ,  G01N2021/7709 ,  G01N2021/7786 ,  Y10S435/808 ,  Y10S436/805

摘要： The invention concerns a device and a method for carrying out fluorescence immunoassays, wherein from at least one light source light is directed onto a surface at one end of a light waveguide and with the light coupled into the light waveguide by evanescent field excitation at the surface of the light waveguide fluorescence of at least one labelling substance bound to a chemical or biochemical partner of a general receptor-ligand system is excited. The solution according to the invention is here to provide a possible way of carrying out fluorescence immunoassays with high accuracy of measurement at low cost and within a short time. To achieve this object, the fluorescent light is decoupled from the light waveguide and directed via an optical system onto an optical detector with which the intensity of the fluorescent light is measured. Here the light waveguide is held in a measuring chamber formed in a piston of a piston and cylinder unit, and the measuring chamber is connected to the interior of the cylinder by an inlet formed in the piston.

摘要翻译： 本发明涉及用于进行荧光免疫测定的装置和方法，其中从至少一个光源光引导到光波导的一端的表面上，并且通过在表面处的ev逝场激发耦合到光波导中的光 激发了与一般受体 - 配体体系的化学或生物化学配偶体结合的至少一种标记物质的光波导荧光。 本发明的解决方案在此提供以低成本和短时间内具有高精度测量的荧光免疫测定的可能方式。 为了实现该目的，荧光从光波导解耦并通过光学系统被引导到测量荧光的强度的光学检测器上。 这里，光波导保持在形成在活塞和气缸单元的活塞中的测量室中，并且测量室通过形成在活塞中的入口连接到气缸的内部。