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1.发明授权Method for performing site-specific affinity fractionation for use in DNA sequencing 失效用于DNA测序的用于进行位点特异性亲和分离的方法
公开(公告)号：US5905024A
公开(公告)日：1999-05-18
申请号：US768893
申请日：1996-12-17
CPC分类号： B01L3/0255 , B01L3/0244 , B01L3/5085 , B82Y30/00 , B01J2219/00387 , B01J2219/00585 , B01J2219/00596 , B01J2219/00608 , B01J2219/0061 , B01J2219/00617 , B01J2219/00626 , B01J2219/00644 , B01J2219/00659 , B01J2219/00662 , B01J2219/00677 , B01J2219/00722 , B01L2200/12 , B01L2300/069 , B01L2300/0893 , B01L2300/0896 , B01L2300/1822 , B01L2300/1888 , C40B40/06 , C40B60/14 , Y10S436/80 , Y10S436/807 , Y10S436/809 , Y10T436/143333
摘要： A method for fractionating and sequencing DNA via affinity interaction is provided comprising contacting cleaved DNA to a first array of oligonucleotide molecules to facilitate hybridization between said cleaved DNA and the molecules; extracting the hybridized DNA from the molecules; contacting said extracted hybridized DNA with a second array of oligonucleotide molecules, wherein the oligonucleotide molecules in the second array have specified base sequences that are complementary to said extracted hybridized DNA; and attaching labeled DNA to the second array of oligonucleotide molecules, wherein the labeled re-hybridized DNA have sequences that are complementary to the oligomers. The invention further provides a method for performing multi-step conversions of the chemical structure of compounds comprising supplying an array of polyacrylamide vessels separated by hydrophobic surfaces; immobilizing a plurality of reactants, such as enzymes, in the vessels so that each vessel contains one reactant; contacting the compounds to each of the vessels in a predetermined sequence and for a sufficient time to convert the compounds to a desired state; and isolating the converted compounds from said array.
摘要翻译： 提供了通过亲和相互作用对DNA进行分级和测序的方法，包括将切割的DNA与第一阵列的寡核苷酸分子接触以促进所述切割的DNA与分子之间的杂交; 从分子中提取杂交的DNA; 使所述提取的杂交DNA与第二阵列的寡核苷酸分子接触，其中所述第二阵列中的寡核苷酸分子具有与所述提取的杂交DNA互补的特定碱基序列; 并将标记的DNA附着到第二寡核苷酸分子阵列上，其中标记的再杂交DNA具有与寡聚物互补的序列。 本发明还提供了一种用于进行化合物化学结构的多步转化的方法，包括提供由疏水性表面分离的聚丙烯酰胺容器阵列; 将诸如酶的多种反应物固定在容器中，使得每个容器含有一种反应物; 以预定顺序将化合物与每个容器接触并持续足够的时间将化合物转化为所需状态; 并从所述阵列中分离转化的化合物。
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2.发明授权Miniaturized reaction vessel system, method for performing site-specific biochemical reactions and affinity fractionation for use in DNA sequencing 失效小型化反应容器系统，用于进行位点特异性生化反应的方法和用于DNA测序的亲和分离
公开(公告)号：US06143499A
公开(公告)日：2000-11-07
申请号：US99959
申请日：1998-06-19
CPC分类号： B01L3/0255 , B01L3/0244 , B01L3/5085 , B82Y30/00 , B01J2219/00387 , B01J2219/00585 , B01J2219/00596 , B01J2219/00608 , B01J2219/0061 , B01J2219/00617 , B01J2219/00626 , B01J2219/00644 , B01J2219/00659 , B01J2219/00662 , B01J2219/00677 , B01J2219/00722 , B01L2200/12 , B01L2300/069 , B01L2300/0893 , B01L2300/0896 , B01L2300/1822 , B01L2300/1888 , C40B40/06 , C40B60/14 , Y10S436/80 , Y10S436/807 , Y10S436/809 , Y10T436/143333
摘要： A method for fractionating and sequencing DNA via affinity interaction is provided comprising contacting cleaved DNA to a first array of oligonucleotide molecules to facilitate hybridization between said cleaved DNA and the molecules; extracting the hybridized DNA from the molecules; contacting said extracted hybridized DNA with a second array of oligonucleotide molecules, wherein the oligonucleotide molecules in the second array have specified base sequences that are complementary to said extracted hybridized DNA; and attaching labeled DNA to the second array of oligonucleotide molecules, wherein the labeled re-hybridized DNA have sequences that are complementary to the oligomers. The invention further provides a method for performing multi-step conversions of the chemical structure of compounds comprising supplying an array of polyacrylamide vessels separated by hydrophobic surfaces; immobilizing a plurality of reactants, such as enzymes, in the vessels so that each vessel contains one reactant; contacting the compounds to each of the vessels in a predetermined sequence and for a sufficient time to convert the compounds to a desired state; and isolating the converted compounds from said array.
摘要翻译： 提供了通过亲和相互作用对DNA进行分级和测序的方法，包括将切割的DNA与第一阵列的寡核苷酸分子接触以促进所述切割的DNA与分子之间的杂交; 从分子中提取杂交的DNA; 使所述提取的杂交DNA与第二阵列的寡核苷酸分子接触，其中所述第二阵列中的寡核苷酸分子具有与所述提取的杂交DNA互补的特定碱基序列; 并将标记的DNA附着到第二寡核苷酸分子阵列上，其中标记的再杂交DNA具有与寡聚物互补的序列。 本发明还提供了一种用于进行化合物化学结构的多步转化的方法，包括提供由疏水性表面分离的聚丙烯酰胺容器阵列; 将诸如酶的多种反应物固定在容器中，使得每个容器含有一种反应物; 以预定顺序将化合物与每个容器接触并持续足够的时间将化合物转化为所需状态; 并从所述阵列中分离转化的化合物。
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