公开(公告)号：US07253268B2

公开(公告)日：2007-08-07

申请号：US09847513

申请日：2001-05-01

申请人： Edward F. Delong ,  Oded Beja

发明人： Edward F. Delong ,  Oded Beja

IPC分类号： C07M21/02 ,  C07M21/04 ,  C12N15/00 ,  C12N15/09 ,  C12N15/63

CPC分类号： C07K14/195

摘要： A light-driven energy generation system using proteorhodopsin is provided. Proteorhodopsin sequences were retrieved and amplified from naturally occurring members of the domain Bacteria using proteorhodopsin-specific polymerase chain reaction primers. Proteorhodopsin sequences were placed in expression vectors for production of proteorhodopsin proteins in a host, for instance, E. coli and other bacteria. The system also includes a light source and a source of retinal, that allows the system to convert light into biochemical energy. The generated biochemical energy could be mediated into electrical energy by a mediator.

摘要翻译： 提供了使用蛋白质视紫红质的光驱能量生成系统。 使用蛋白质视紫红质特异性聚合酶链反应引物从天然存在的细菌成员中检索和扩增蛋白质视紫红质序列。 将蛋白质视紫红质序列置于用于在宿主例如大肠杆菌和其他细菌中产生蛋白质视紫红质蛋白的表达载体中。 该系统还包括光源和视网膜源，允许系统将光转化为生化能。 生成的生化能量可以通过介体介导到电能中。

公开(公告)号：US06143499A

公开(公告)日：2000-11-07

申请号：US99959

申请日：1998-06-19

申请人： Andrei Darievich Mirzabekov ,  Yuri Petrovich Lysov ,  Svetlana A. Dubley

发明人： Andrei Darievich Mirzabekov ,  Yuri Petrovich Lysov ,  Svetlana A. Dubley

IPC分类号： B01L3/00 ,  B01L3/02 ,  C12Q1/68 ,  C40B40/06 ,  C40B60/14 ,  C12P19/34 ,  C07M21/02 ,  C07M21/04

CPC分类号： B01L3/0255 ,  B01L3/0244 ,  B01L3/5085 ,  B82Y30/00 ,  B01J2219/00387 ,  B01J2219/00585 ,  B01J2219/00596 ,  B01J2219/00608 ,  B01J2219/0061 ,  B01J2219/00617 ,  B01J2219/00626 ,  B01J2219/00644 ,  B01J2219/00659 ,  B01J2219/00662 ,  B01J2219/00677 ,  B01J2219/00722 ,  B01L2200/12 ,  B01L2300/069 ,  B01L2300/0893 ,  B01L2300/0896 ,  B01L2300/1822 ,  B01L2300/1888 ,  C40B40/06 ,  C40B60/14 ,  Y10S436/80 ,  Y10S436/807 ,  Y10S436/809 ,  Y10T436/143333

摘要： A method for fractionating and sequencing DNA via affinity interaction is provided comprising contacting cleaved DNA to a first array of oligonucleotide molecules to facilitate hybridization between said cleaved DNA and the molecules; extracting the hybridized DNA from the molecules; contacting said extracted hybridized DNA with a second array of oligonucleotide molecules, wherein the oligonucleotide molecules in the second array have specified base sequences that are complementary to said extracted hybridized DNA; and attaching labeled DNA to the second array of oligonucleotide molecules, wherein the labeled re-hybridized DNA have sequences that are complementary to the oligomers. The invention further provides a method for performing multi-step conversions of the chemical structure of compounds comprising supplying an array of polyacrylamide vessels separated by hydrophobic surfaces; immobilizing a plurality of reactants, such as enzymes, in the vessels so that each vessel contains one reactant; contacting the compounds to each of the vessels in a predetermined sequence and for a sufficient time to convert the compounds to a desired state; and isolating the converted compounds from said array.

摘要翻译： 提供了通过亲和相互作用对DNA进行分级和测序的方法，包括将切割的DNA与第一阵列的寡核苷酸分子接触以促进所述切割的DNA与分子之间的杂交; 从分子中提取杂交的DNA; 使所述提取的杂交DNA与第二阵列的寡核苷酸分子接触，其中所述第二阵列中的寡核苷酸分子具有与所述提取的杂交DNA互补的特定碱基序列; 并将标记的DNA附着到第二寡核苷酸分子阵列上，其中标记的再杂交DNA具有与寡聚物互补的序列。 本发明还提供了一种用于进行化合物化学结构的多步转化的方法，包括提供由疏水性表面分离的聚丙烯酰胺容器阵列; 将诸如酶的多种反应物固定在容器中，使得每个容器含有一种反应物; 以预定顺序将化合物与每个容器接触并持续足够的时间将化合物转化为所需状态; 并从所述阵列中分离转化的化合物。

公开(公告)号：US6048696A

公开(公告)日：2000-04-11

申请号：US78290

申请日：1998-05-13

申请人： Leslie M. Hoffman ,  Gregory A. Hawkins

发明人： Leslie M. Hoffman ,  Gregory A. Hawkins

IPC分类号： C12Q1/68 ,  C12P19/34 ,  C07K13/00 ,  C07M21/02

CPC分类号： C12Q1/701 ,  C12Q2600/156

摘要： A method of analyzing a DNA molecule is disclosed. In one embodiment the method comprises the steps of exposing a DNA molecule to an effective amount of a chemical modification reagent wherein the reagent converts guanine to 8-hydroxyguanine. The oxidized product is then exposed to a DNA glycosylase enzyme and the DNA molecule is cleaved at the site of the 8-hydroxyguanine. The fragments are then resolved by electrophoresis and the position of guanine residues within the DNA molecule is determined. In a preferred embodiment of the present invention, the modification reagent is a thiazine dye and the enzyme is FPG protein.

摘要翻译： 公开了分析DNA分子的方法。 在一个实施方案中，该方法包括将DNA分子暴露于有效量的化学修饰试剂的步骤，其中试剂将鸟嘌呤转化为8-羟基鸟嘌呤。 然后将氧化的产物暴露于DNA糖基化酶，并且DNA分子在8-羟基鸟嘌呤的位点处被切割。 然后通过电泳解析片段，并确定DNA分子内鸟嘌呤残基的位置。 在本发明的优选实施方案中，修饰试剂是噻嗪染料，酶是FPG蛋白。

公开(公告)号：US6025480A

公开(公告)日：2000-02-15

申请号：US415655

申请日：1995-04-03

申请人： Joan Massague ,  Mong-Hong Lee

发明人： Joan Massague ,  Mong-Hong Lee

IPC分类号： A61K38/00 ,  C07K14/47 ,  C12N1/21 ,  C07M21/02 ,  C12N15/70 ,  C02H21/04

CPC分类号： C07K14/4738 ,  A61K38/00 ,  C12N2799/021

摘要： This invention provides an isolated nucleic acid molecule encoding a mammalian p57.sup.KIP2. This invention also provides vectors comprising the isolated nucleic acid molecule encoding a mammalian p57.sup.KIP2. This invention further provides a host vector system for the production of a mammalian p57.sup.KIP2. This invention also provides probes for the isolated nucleic acid molecule encoding a mammalian p57.sup.KIP2. This invention provides antibodies directed against a mammalian p57.sup.KIP2. This invention also provides transgenic animals comprising isolated nucleic acid molecules encoding a mammalian p57.sup.KIP2. Finally, this invention provides different uses of the mammalian p57.sup.KIP2.

摘要翻译： 本发明提供了编码哺乳动物p57KIP2的分离的核酸分子。 本发明还提供了包含编码哺乳动物p57KIP2的分离的核酸分子的载体。 本发明还提供了用于产生哺乳动物p57KIP2的宿主载体系统。 本发明还提供了编码哺乳动物p57KIP2的分离的核酸分子的探针。 本发明提供针对哺乳动物p57KIP2的抗体。 本发明还提供包含编码哺乳动物p57KIP2的分离的核酸分子的转基因动物。 最后，本发明提供哺乳动物p57KIP2的不同用途。