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公开(公告)号：US4816168A
公开(公告)日：1989-03-28
申请号：US923909
申请日：1986-10-28
CPC分类号： G01N33/491 , Y10T436/25375
摘要： This invention is directed to means for inhibiting the apparent shift in the buoyant density of and/or to restore any loss in the buoyant density of the granulocytic white blood cells in a sample of blood, thereby insuring the quality of the separation of lymphocytes and monocytes from granulocytes in a blood sample. The invention comprises contacting a blood sample with a hypertonic fluid containing a low molecular weight organic or inorganic ionic substance and/or with an isotonic or hypertonic fluid containing a high molecular weight organic substance which may contain organic molecules having a lipophilic substituent in their structures and/or with a culture medium for blood cells. An integral part of the invention is an improved blood separation tube utilizing a gel-like substance having a specific gravity between 1.060-1.065 g/cm.sup.3 to significantly enhance the purity of cell separation, while providing acceptable cell yields.
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公开(公告)号：US4640785A
公开(公告)日：1987-02-03
申请号：US685801
申请日：1984-12-24
CPC分类号： G01N33/491 , Y10T436/25375
摘要： This invention is directed to means for inhibiting the apparent shift in the buoyant density of and/or to restore any loss in the buoyant density of the granulocytic white blood cells in a sample of blood, thereby insuring the quality of the separation of lymphocytes and monocytes from granulocytes in a blood sample. The invention comprises contacting a blood sample with a hypertonic fluid containing a low molecular weight organic or inorganic ionic substance and/or with an isotonic or hypertonic fluid containing a high molecular weight organic substance which may contain organic molecules having a lipophilic substituent in their structures and/or with a culture medium for blood cells. An integral part of the invention is an improved blood separation tube utilizing a gel-like substance having a specific gravity between 1.060-1.065 g/cm.sup.3 to significantly enhance the purity of cell separation, while providing acceptable cell yields.
摘要翻译： 本发明涉及用于抑制浮力密度的明显变化和/或恢复血液样品中粒细胞白细胞的浮力密度的任何损失的手段，从而确保淋巴细胞和单核细胞分离的质量 来自血液样品中的粒细胞。 本发明包括使血液样品与含有低分子量有机或无机离子物质的高渗流体和/或含有高分子量有机物质的等渗或高渗流体接触，所述高分子量或高渗流体可以含有在其结构中具有亲脂性取代基的有机分子， /或用于血细胞的培养基。 本发明的一个组成部分是利用比重在1.060-1.065g / cm 3之间的凝胶状物质的改进的血液分离管，以显着提高细胞分离的纯度，同时提供可接受的细胞产量。
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公开(公告)号：US4021340A
公开(公告)日：1977-05-03
申请号：US636127
申请日：1975-11-28
申请人： Anthony R. Zine, Jr.
发明人： Anthony R. Zine, Jr.
CPC分类号： G01N33/491
摘要： Gel-like compositions useful for separating and partitioning whole blood into serum and clot portions. The composition comprises a polybutene liquid polymer having an inert high surface area inorganic filler dispersed therein, the filler being present in an amount sufficient to impart to the composition a specific gravity ranging from about 1.030 to about 1.090 and a viscosity ranging from about 200,000 to about 850,000 centistokes. When centrifuged in the presence of whole blood, the composition forms a chemical and physical barrier between the serum and clot portions.
摘要翻译： 用于将全血分离和分配成血清和凝块部分的凝胶状组合物。 该组合物包含其中分散有惰性高表面积无机填料的聚丁烯液体聚合物，该填料以足以使组合物赋予比重为约1.030至约1.090，粘度范围为约200,000至约 85万厘沲。 当在全血存在下离心时，组合物在血清和血块部分之间形成化学和物理屏障。
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公开(公告)号：US4083784A
公开(公告)日：1978-04-11
申请号：US791921
申请日：1977-04-28
申请人： Anthony R. Zine, Jr.
发明人： Anthony R. Zine, Jr.
CPC分类号： G01N33/491 , C08L83/04 , C08G77/14 , C08G77/46 , Y10T436/107497
摘要： Gel-like composition useful for separating and partitioning whole blood into serum and clot portions. The composition has a specific gravity between that of the serum and clot portions such that, when centrifuged in the presence of whole blood, the composition forms a chemical and physical barrier between the serum and clot portions. The composition comprises, in combination, a silicone fluid, an inert siliceous filler dispersed therein, and a network former consisting of a polysiloxane-polyoxyalkyl copolymer which stabilizes the composition by minimizing and/or avoiding "wet out" of the siliceous filler with time. The composition has a viscosity within the range of about 200,000 to about 600,000 centistokes, preferably within the range of about 350,000 to about 450,000 centistokes.
摘要翻译： 凝胶状组合物，用于将全血分离和分配成血清和凝块部分。 该组合物具有在血清和凝块部分之间的比重，使得当在全血存在下离心时，组合物在血清和凝块部分之间形成化学和物理屏障。 组合物组合物包含硅氧烷流体，其中分散的惰性硅质填料和由聚硅氧烷 - 聚氧烷基共聚物组成的网络形成剂，其通过使硅酸盐填料随时间的“湿”最小化和/或避免使得组合物稳定。 组合物的粘度在约200,000至约600,000厘沲的范围内，优选在约35万至约45万厘沲的范围内。
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公开(公告)号：US4046699A
公开(公告)日：1977-09-06
申请号：US737536
申请日：1976-11-01
申请人： Anthony R. Zine, Jr.
发明人： Anthony R. Zine, Jr.
CPC分类号： G01N33/491
摘要： In apparatus for effecting a sealed separation or partition between two phases of a multiphase liquid upon centrifugation of the multiphase liquid, a device which facilitates access through the partition to a phase of a separated multiphase liquid which is confined between the partition and a lower or closed end of a vessel retaining such liquid.
摘要翻译： 在用于在多相液体离心时进行多相液体的两相之间的密封分离或隔离的装置中，一种有助于通过分隔件进入到分离的多相液体的相的装置，所述分离的多相液体被限制在隔板与下部或闭合 保留这种液体的容器的末端。
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公开(公告)号：US4189382A
公开(公告)日：1980-02-19
申请号：US892992
申请日：1978-04-03
申请人： Anthony R. Zine, Jr.
发明人： Anthony R. Zine, Jr.
CPC分类号： G01N33/491
摘要： Methods and apparatus for separating blood which utilize suspendable contact-activating particles to initiate blood clotting generally throughout the volume of blood are disclosed herein. The contact-activating particles are deposited in a vessel; and when whole blood is supplied to the vessel, the particles are suspended throughout the volume of the blood and remain so suspended, without mechanical agitation, during at least the initial stages of coagulation. The particles are precipitated from the serum portion of the blood during a subsequent centrifugal separation. Also, a process for making appropriately sized contact-activating particles is disclosed.
摘要翻译： 本文公开了用于分离血液的方法和装置，其利用可悬浮的接触激活颗粒以通常在整个体积的血液中引发血液凝固。 接触激活颗粒沉积在容器中; 并且当将全血供应到血管时，在至少凝固的初始阶段期间，颗粒悬浮在血液的整个体积中并保持如此悬浮，无机械搅动。 在随后的离心分离期间，颗粒从血液的血清部分沉淀。 另外，公开了适当尺寸的接触激活粒子的制造方法。
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公开(公告)号：US4049692A
公开(公告)日：1977-09-20
申请号：US532946
申请日：1974-12-16
申请人： Anthony R. Zine, Jr.
发明人： Anthony R. Zine, Jr.
CPC分类号： G01N33/491 , C08L83/04 , C08G77/14 , C08G77/46 , Y10T436/107497
摘要： Gel-like composition useful for separating and partitioning whole blood into serum and clot portions. The composition has a specific gravity between that of the serum and clot portions such that, when centrifuged in the presence of whole blood, the composition forms a chemical and physical barrier between the serum and clot portions. The composition comprises, in combination, a silicone fluid, an inert siliceous filler dispersed therein, and a network former consisting of a polysiloxane-polyoxyalkyl copolymer which stabilizes the composition by minimizing and/or avoiding "wet out" of the siliceous filler with time. The composition has a viscosity within the range of about 200,000 to about 600,000 centistokes, preferably within the range of about 350,000 to about 450,000 centistokes.
摘要翻译： 凝胶状组合物，用于将全血分离和分配成血清和凝块部分。 该组合物具有在血清和凝块部分之间的比重，使得当在全血存在下离心时，组合物在血清和凝块部分之间形成化学和物理屏障。 组合物组合物包含硅氧烷流体，其中分散的惰性硅质填料和由聚硅氧烷 - 聚氧烷基共聚物组成的网络形成剂，其通过使硅酸盐填料随时间的“湿”最小化和/或避免使得组合物稳定。 组合物的粘度在约200,000至约600,000厘沲的范围内，优选在约35万至约45万厘沲的范围内。
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公开(公告)号：US4333564A
公开(公告)日：1982-06-08
申请号：US112684
申请日：1980-01-16
CPC分类号： G01N33/491 , B01D43/00 , G01N33/48
摘要： The rheological properties of thixotropic, gel-like compositions employed in blood separation tubes are controlled by incorporating in the composition up to about five percent by weight, based on the amount of composition, of a disilazane.
摘要翻译： 用于血液分离管中的触变性凝胶状组合物的流变学性质通过在组合物中加入高达约5重量％（基于组合物的量）的二硅氮烷来控制。
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9.发明授权
公开(公告)号：US4190535A
公开(公告)日：1980-02-26
申请号：US922825
申请日：1978-07-10
CPC分类号： G01N33/491
摘要： This invention relates to a method for isolating platelets, lymphocytes, and monocytes from anticoagulated blood. The method involves centrifuging at high force a sample of uncoagulated blood with a water-insoluble thixotropic gel-like material having a specific gravity between 1.065-1.077 g/cc for a length of time sufficient to cause the gel-like material to form a barrier between the platelets, lymphocytes, monocytes, and plasma fraction of the blood and the heavier blood cells. Thereafter, the plasma is withdrawn and the platelets, lymphocytes, and monocytes removed from above the barrier in a buffer solution. Where desired, the platelets can be separated from the lymphocytes and monocytes utilizing a similar process but wherein the water-insoluble thixotropic gel-like material has a specific gravity of less than about 1.055 g/cc.
摘要翻译： 本发明涉及从抗凝血中分离血小板，淋巴细胞和单核细胞的方法。 该方法包括以非常高的力将具有1.065-1.077g / cc之间的比重的水不溶性触变凝胶状材料的非离子的血液样品离心一段足以使凝胶状材料形成屏障的时间 在血小板，淋巴细胞，单核细胞和血液和较重血细胞的血浆级分之间。 此后，取出血浆，将血小板，淋巴细胞和单核细胞从缓冲溶液中的屏障上方除去。 如果需要，可以使用类似的方法将血小板与淋巴细胞和单核细胞分离，但其中水不溶性触变凝胶状材料的比重小于约1.055g / cc。
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