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1.发明授权公开(公告)号：US07144580B2
公开(公告)日：2006-12-05
申请号：US11235982
申请日：2005-09-27
IPC分类号： A61K39/102 , A61K39/02 , A61K39/116 , A61K38/00 , C07K1/00
CPC分类号： C07K14/285 , Y10S530/825
摘要： Vaccines and methods against M. haemolytica infections in cattle. The vaccine compositions include a recombinant outer membrane protein of M. haemolytica designated PlpE and/or subunits thereof, alone or in combination with other antigenic components, and a carrier or diluent. The methods involve administering an effective immunizing amount of the vaccines to susceptible bovine.
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公开(公告)号：US20120241590A1
公开(公告)日：2012-09-27
申请号：US13069928
申请日：2011-03-23
IPC分类号： H01L27/144 , H01J9/227 , B05D5/06
CPC分类号： H01L27/14625 , H01L27/14685
摘要： Spectroscopic filter arrays and methods for making spectroscopic filter arrays are provided. The arrays are formed using a dispersion of filter particles having selected moieties attached to the surface of the particles and a microarray having complementary moieties formed in an array on a substrate, such that each filter particle is attached to a selected region of the microarray. The moiety on the substrate may be RNA or DNA or other molecule. The substrate may be a surface of a photodetector array, a transparent plate that may be placed in registration with the photodetector or a stamp used to transfer the filter array to a photodetector array.
摘要翻译： 提供光谱过滤器阵列和制造分光过滤器阵列的方法。 使用具有连接到颗粒表面的选定部分的过滤颗粒的分散体和在基底上形成有阵列的互补部分的微阵列形成阵列，使得每个过滤颗粒附着到微阵列的选定区域。 底物上的部分可以是RNA或DNA或其他分子。 基板可以是光电检测器阵列的表面，可以放置成与光电检测器对准的透明板或用于将滤光器阵列传送到光电检测器阵列的印模。
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公开(公告)号：US07361465B2
公开(公告)日：2008-04-22
申请号：US10935534
申请日：2004-09-07
申请人： George L. Murphy , J. Penn Whitley
发明人： George L. Murphy , J. Penn Whitley
CPC分类号： C12P19/34 , C12Q1/6806 , C12Q2525/204 , C12Q2525/173 , C12Q2527/137 , C12Q2521/325
摘要： The present invention provides methods and compositions that enable tagging and amplification of targeted RNA molecules. A targeted RNA molecule is any non-polyadenylated RNA molecule including, for example, miRNA, siRNA, rRNA, tRNA, synthetic RNA, or non-polyadenylated mRNA such as mRNA from bacteria. In certain aspects, the invention provides methods and compositions for the genome-wide expression analysis of bacterial genes. Significantly, the methods enable genome-wide expression analysis in circumstances where bacterial numbers were previously too low to purify adequate amounts of RNA for DNA microarray analysis or other applications. Such methods are particularly useful for the study of bacterial gene expression during host-cell infection. The invention also provides kits for tagging and amplifying targeted RNA molecules.
摘要翻译： 本发明提供能够标记和扩增靶RNA的分子的方法和组合物。 靶向RNA分子是任何非聚腺苷酸化的RNA分子，包括例如miRNA，siRNA，rRNA，tRNA，合成的RNA或非聚腺苷酸化的mRNA，例如来自细菌的mRNA。 在某些方面，本发明提供了用于细菌基因的全基因组表达分析的方法和组合物。 重要的是，在细菌数量先前太低而无法纯化足够量的RNA用于DNA微阵列分析或其他应用的情况下，这些方法能够进行全基因组表达分析。 这种方法对宿主细胞感染期间细菌基因表达的研究特别有用。 本发明还提供用于标记和扩增靶向RNA分子的试剂盒。
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公开(公告)号：US06994963B1
公开(公告)日：2006-02-07
申请号：US09613535
申请日：2000-07-10
CPC分类号： C12N15/1027 , C12P19/34
摘要： This invention pertains to a method for generating a pool of nucleic acid fragments useful for in vitro recombination and the creation of novel DNA sequences that encode desirable proteins or enzymes. The invention provides a defined mixture of nucleic acids and methods for use in the synthesis, mutagenesis, and recombination of nucleic acids. Nucleic acids may be synthesized by creating a nucleic acid extension ladder, annealing the extension ladder to template nucleic acids, and further extending the ladder of nucleic acids. The invention also relates to methods for performing repeated cycles of synthesis for the purpose of mutagenesis or recombination, methods for producing mutant peptides and proteins from the mutagenized or recombined nucleic acids, and methods for selecting a peptide, polypeptide or protein having altered biological activities.
摘要翻译： 本发明涉及用于产生用于体外重组的核酸片段库的集合，以及产生编码所需蛋白质或酶的新型DNA序列的方法。 本发明提供用于核酸的合成，诱变和重组的核酸和方法的限定混合物。 可以通过产生核酸延伸梯，将延伸梯退火至模板核酸并进一步延长核酸梯级来合成核酸。 本发明还涉及用于进行突变或重组的重复合成循环的方法，用于从诱变或重组的核酸产生突变型肽和蛋白质的方法，以及用于选择具有改变的生物活性的肽，多肽或蛋白质的方法。
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公开(公告)号：US20180051390A1
公开(公告)日：2018-02-22
申请号：US15791403
申请日：2017-10-23
CPC分类号： C40B50/18 , B01J19/0046 , B01J2219/00382 , B01J2219/00576 , B01J2219/00637 , B01J2219/00648 , B01J2219/00722
摘要： Analyte filter arrays and methods for making an analyte filter array are provided. The arrays are formed using a dispersion of filter particles having selected moieties attached to the surface of the particles and a microarray having complementary moieties formed in an array on a substrate, such that each filter particle is attached to a selected region of the microarray. The moiety on the substrate may be RNA or DNA or other molecule. The substrate may be a surface of a detector array, a membrane that may be placed in registration with the detector array or a stamp used to transfer the filter array to a detector array.
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公开(公告)号：US09828696B2
公开(公告)日：2017-11-28
申请号：US13070077
申请日：2011-03-23
CPC分类号： C40B50/18 , B01J19/0046 , B01J2219/00382 , B01J2219/00576 , B01J2219/00637 , B01J2219/00648 , B01J2219/00722
摘要： Analyte filter arrays and methods for making an analyte filter array are provided. The arrays are formed using a dispersion of filter particles having selected moieties attached to the surface of the particles and a microarray having complementary moieties formed in an array on a substrate, such that each filter particle is attached to a selected region of the microarray. The moiety on the substrate may be RNA or DNA or other molecule. The substrate may be a surface of a detector array, a membrane that may be placed in registration with the detector array or a stamp used to transfer the filter array to a detector array.
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公开(公告)号：US10550494B2
公开(公告)日：2020-02-04
申请号：US15791403
申请日：2017-10-23
摘要： Analyte filter arrays and methods for making an analyte filter array are provided. The arrays are formed using a dispersion of filter particles having selected moieties attached to the surface of the particles and a microarray having complementary moieties formed in an array on a substrate, such that each filter particle is attached to a selected region of the microarray. The moiety on the substrate may be RNA or DNA or other molecule. The substrate may be a surface of a detector array, a membrane that may be placed in registration with the detector array or a stamp used to transfer the filter array to a detector array.
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公开(公告)号：US09252175B2
公开(公告)日：2016-02-02
申请号：US13069928
申请日：2011-03-23
CPC分类号： H01L27/14625 , H01L27/14685
摘要： Spectroscopic filter arrays and methods for making spectroscopic filter arrays are provided. The arrays are formed using a dispersion of filter particles having selected moieties attached to the surface of the particles and a microarray having complementary moieties formed in an array on a substrate, such that each filter particle is attached to a selected region of the microarray. The moiety on the substrate may be RNA or DNA or other molecule. The substrate may be a surface of a photodetector array, a transparent plate that may be placed in registration with the photodetector or a stamp used to transfer the filter array to a photodetector array.
摘要翻译： 提供光谱过滤器阵列和制造分光过滤器阵列的方法。 使用具有连接到颗粒表面的选定部分的过滤颗粒的分散体和在基底上形成有阵列的互补部分的微阵列形成阵列，使得每个过滤颗粒附着到微阵列的选定区域。 底物上的部分可以是RNA或DNA或其他分子。 基板可以是光电检测器阵列的表面，可以放置成与光电检测器对准的透明板或用于将滤光器阵列传送到光电检测器阵列的印模。
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公开(公告)号：US20120245055A1
公开(公告)日：2012-09-27
申请号：US13070077
申请日：2011-03-23
CPC分类号： C40B50/18 , B01J19/0046 , B01J2219/00382 , B01J2219/00576 , B01J2219/00637 , B01J2219/00648 , B01J2219/00722
摘要： Analyte filter arrays and methods for making an analyte filter array are provided. The arrays are formed using a dispersion of filter particles having selected moieties attached to the surface of the particles and a microarray having complementary moieties formed in an array on a substrate, such that each filter particle is attached to a selected region of the microarray. The moiety on the substrate may be RNA or DNA or other molecule. The substrate may be a surface of a detector array, a membrane that may be placed in registration with the detector array or a stamp used to transfer the filter array to a detector array.
摘要翻译： 提供了分析物过滤器阵列和用于制备分析物过滤器阵列的方法。 使用具有连接到颗粒表面的选定部分的过滤颗粒的分散体和在基底上形成有阵列的互补部分的微阵列形成阵列，使得每个过滤颗粒附着到微阵列的选定区域。 底物上的部分可以是RNA或DNA或其他分子。 衬底可以是检测器阵列的表面，可以放置成与检测器阵列对准的膜或用于将过滤器阵列传送到检测器阵列的标记。
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