公开(公告)号：US20170326548A1

公开(公告)日：2017-11-16

申请号：US15603128

申请日：2017-05-23

申请人： Taiwan Semiconductor Manufacturing Company, Ltd.

发明人： Shang-Ying Tsai ,  Li-Min Hung ,  Jung-Huei Peng

IPC分类号： B01L3/00 ,  B32B37/30 ,  B82Y30/00

CPC分类号： B01L3/502707 ,  B01J2219/00286 ,  B01J2219/00382 ,  B01J2219/00637 ,  B01L3/502715 ,  B01L2200/12 ,  B01L2300/0636 ,  B01L2300/0877 ,  B01L2300/0887 ,  B01L2300/0896 ,  B01L2300/12 ,  B32B37/30 ,  B32B2307/412 ,  B82Y30/00

摘要： The present disclosure provides flow cells and methods of fabricating flow cells. The method includes combining three portions: a first substrate, a second substrate, and microfluidic channels between the first substrate and the second substrate having walls of a photoresist dry film. Through-holes for inlet and outlet are formed in the first substrate or the second substrate. Patterned capture sites are stamped on the first substrate and the second substrate by a nanoimprint lithography process. In other embodiments, parts of the patterned capture sites are selectively attached to a surface chemistry pattern formed of silicon oxide islands each disposed on an outcrop of a soft bottom layer.

公开(公告)号：US09481945B2

公开(公告)日：2016-11-01

申请号：US13597362

申请日：2012-08-29

申请人： David Juncker ,  Huiyan Li

发明人： David Juncker ,  Huiyan Li

IPC分类号： C40B60/02 ,  G01N33/543 ,  G01N33/68 ,  C40B20/02 ,  B01L3/00

CPC分类号： C40B60/02 ,  B01J2219/00382 ,  B01J2219/00533 ,  B01J2219/00704 ,  B01J2219/00725 ,  B01J2219/0074 ,  B01L3/50853 ,  B01L3/5088 ,  B01L3/563 ,  B01L2200/0642 ,  B01L2200/16 ,  B01L2300/0819 ,  B01L2300/0822 ,  C40B20/02 ,  G01N33/54366 ,  G01N33/6845

摘要： Rapid and specific detection of biological cells and biomolecules is important to biological assays across diverse fields including genomics, proteomics, diagnoses, and pathological studies. Microarrays and microfluidics increasingly dominate such detection techniques due to the ability to perform significant numbers of tests with limited sample volumes. A snap chip assembly is provided for the transfer of a microarray of reagents within semi-spherical liquid droplets on a transfer chip to a target assay microarray on an assay chip following assembly of the two chips and physical contact of the droplets with the target array. Reagents in nanoliter quantities are spotted on both chips and selectively transferred as liquid droplets between transfer chip and assay chip within the contact areas. Using the snap chip structure the inventors performed immunoassays with colocalization of capture and detection antibodies with 10 targets and bead-in-gel droplet microarrays with 9 targets in the low pg/ml regime.

摘要翻译： 生物细胞和生物分子的快速和特异性检测对于包括基因组学，蛋白质组学，诊断和病理学研究在内的不同领域的生物测定是重要的。 由于能够以有限的样品量执行大量测试，因此微阵列和微流体学越来越多地主导这种检测技术。 提供了一种快速芯片组件，用于在组装两个芯片之后将微球阵列的转移芯片上的半球形液滴转移到测定芯片上的靶分析微阵列上，并将液滴与靶阵列物理接触。 在两个芯片上都发现纳升量的试剂，并在接触区域内作为转移芯片和测定芯片之间的液滴选择性地转移。 使用快速芯片结构，本发明人使用具有10个靶的凝胶捕获和检测抗体进行免疫测定，并在低pg / ml方案中用9个靶标进行珠凝胶液滴微阵列。

公开(公告)号：US09352315B2

公开(公告)日：2016-05-31

申请号：US14040303

申请日：2013-09-27

申请人： Taiwan Semiconductor Manufacturing Company, Ltd.

发明人： Shang-Ying Tsai ,  Li-Min Hung ,  Jung-Huei Peng

IPC分类号： G01N15/06 ,  G01N33/00 ,  G01N33/48 ,  B82Y10/00 ,  B82Y30/00 ,  B01L3/00

CPC分类号： B01L3/502707 ,  B01J2219/00286 ,  B01J2219/00382 ,  B01J2219/00637 ,  B01L3/502715 ,  B01L2200/12 ,  B01L2300/0636 ,  B01L2300/0877 ,  B01L2300/0887 ,  B01L2300/0896 ,  B01L2300/12 ,  B32B37/30 ,  B32B2307/412 ,  B82Y30/00

摘要： The present disclosure provides flow cells and methods of fabricating flow cells. The method includes combining three portions: a first substrate, a second substrate, and microfluidic channels between the first substrate and the second substrate having walls of a photoresist dry film. Through-holes for inlet and outlet are formed in the first substrate or the second substrate. Patterned capture sites are stamped on the first substrate and the second substrate by a nanoimprint lithography process. In other embodiments, parts of the patterned capture sites are selectively attached to a surface chemistry pattern formed of silicon oxide islands each disposed on an outcrop of a soft bottom layer.

摘要翻译： 本公开提供流动池和制造流动池的方法。 该方法包括在第一基板和具有光致抗蚀剂干膜的壁的第二基板之间组合三个部分：第一基板，第二基板和微流体通道。 用于入口和出口的通孔形成在第一衬底或第二衬底中。 图案化捕获位点通过纳米压印光刻工艺冲压在第一衬底和第二衬底上。 在其他实施例中，图案化捕获位点的部分选择性地附着到由设置在软底层的露头上的氧化硅岛形成的表面化学图案。

公开(公告)号：US20140287959A1

公开(公告)日：2014-09-25

申请号：US14129233

申请日：2012-04-03

申请人： Adam B. Braunschweig ,  Jiajun He ,  Shudan Bian ,  Kevin B. Schesing

发明人： Adam B. Braunschweig ,  Jiajun He ,  Shudan Bian ,  Kevin B. Schesing

IPC分类号： B01J19/00

CPC分类号： B01J19/0046 ,  B01J2219/00382 ,  B01J2219/00387 ,  B01J2219/00527 ,  B01J2219/00576 ,  B01J2219/00585 ,  B01J2219/00612 ,  B01J2219/00626 ,  B01J2219/00632 ,  B01J2219/0065 ,  B01J2219/00659 ,  B82Y15/00

摘要： Polymer Pen Lithography is used to induce bioorthogonal reactions between treated surfaces and functionalized inks create a soft matter layer. Fluorescent and redox-active inks were used to demonstrate that the molecules were immobilized covalently and achieves precise control over ligand orientation and density within each feature. Finally, the utility was demonstrated by creating functional arrays of biologically active probes.

摘要翻译： 聚合物笔平版印刷术用于诱导处理过的表面之间的生物正交反应，功能化油墨产生软质层。 使用荧光和氧化还原活性油墨来证明分子被共价固定，并且在每个特征内实现配体取向和密度的精确控制。 最后，通过创建生物活性探针的功能阵列证明了实用性。

公开(公告)号：US20140272112A1

公开(公告)日：2014-09-18

申请号：US14142121

申请日：2013-12-27

申请人： Intermolecular, Inc.

发明人： Jeroen Van Duren ,  Minh Huu Le ,  Minh Anh Nguyen ,  Sandeep Nijhawan

IPC分类号： C23C14/08 ,  C23C14/22 ,  C23C14/34

CPC分类号： H01L21/30 ,  B01J19/0046 ,  B01J2219/00283 ,  B01J2219/00286 ,  B01J2219/00301 ,  B01J2219/00313 ,  B01J2219/0038 ,  B01J2219/00382 ,  B01J2219/00416 ,  B01J2219/00418 ,  B01J2219/00527 ,  B01J2219/00585 ,  B08B3/04 ,  C23C14/08 ,  C23C14/22 ,  C23C14/34 ,  C23C18/1619 ,  C23C18/1682 ,  C25D17/02 ,  G11C13/0004 ,  H01L21/02104 ,  H01L21/30604 ,  H01L21/67051 ,  H01L21/67057 ,  H01L21/6719 ,  H01L22/10 ,  H01L22/12 ,  H01L22/14 ,  H01L27/2463 ,  H01L45/1608

摘要： Embodiments provided herein describe methods and systems for evaluating electrochromic material processing conditions. A substrate having a plurality of site-isolated regions defined thereon is provided. A first electrochromic material, or a first electrochromic device stack, is formed above a first of the plurality of site-isolated regions using a first set of processing conditions. A second electrochromic material, or a second electrochromic device stack, is formed above a second of the plurality of site-isolated regions using a second set of processing conditions. The second set of processing conditions is different than the first set of processing conditions.

摘要翻译： 本文提供的实施例描述了用于评估电致变色材料加工条件的方法和系统。 提供了具有限定在其上的多个位置隔离区域的基板。 使用第一组处理条件在多个位置隔离区域的第一个上方形成第一电致变色材料或第一电致变色器件堆叠。 使用第二组处理条件在多个位置隔离区域的第二个上方形成第二电致变色材料或第二电致变色器件堆叠。 第二组处理条件与第一组处理条件不同。

公开(公告)号：US08765078B2

公开(公告)日：2014-07-01

申请号：US13358880

申请日：2012-01-26

申请人： Sang Youl Jeon ,  Bo Sung Ku ,  Hee Ju Son

发明人： Sang Youl Jeon ,  Bo Sung Ku ,  Hee Ju Son

IPC分类号： G01N21/01 ,  G01N21/00 ,  G01N33/48 ,  B01L99/00

CPC分类号： B01J19/0046 ,  B01J2219/00317 ,  B01J2219/00382 ,  B01J2219/00387 ,  B01J2219/00509 ,  B01J2219/00743

摘要： There is provided a biochip stamping device. The biochip stamping device includes a stamping jig in which a first biochip is aligned; an inverting mechanism vertically inverting a second biochip; and a movement mechanism transferring the vertically inverted second biochip on the stamping jig to combine the first biochip and the second biochip.

摘要翻译： 提供了一种生物芯片冲压装置。 生物芯片冲压装置包括：冲压夹具，其中第一生物芯片对准; 翻转机构使第二生物芯片垂直翻转; 以及移动机构，其将所述垂直倒置的第二生物芯片传送到所述冲压夹具上，以组合所述第一生物芯片和所述第二生物芯片。

公开(公告)号：US20130324439A1

公开(公告)日：2013-12-05

申请号：US13911012

申请日：2013-06-05

申请人： The Regents of the University of California

发明人： Mark A. LaBarge ,  Chun-Han Lin

IPC分类号： G01N33/50

CPC分类号： G01N33/502 ,  B01J19/0046 ,  B01J2219/00382 ,  B01J2219/00385 ,  B01J2219/00637 ,  B01J2219/00639 ,  B01J2219/00725 ,  B01J2219/0074 ,  B01J2219/00743 ,  G01N33/5008 ,  G01N33/5017

摘要： A combinatorial elastic modulus-modified microenvironment microarray (eMEArray) platform and methods for cell-based functional screening of interactions with combinatorial microenvironments. The platform and methods allow for simultaneous control of the molecular composition and the elastic modulus, and combines the use of microarray and micropatterning technologies. The eMEArrays have been used to show that the microenvironment has effects on drug-cell interactions and contributes to therapeutic response.

摘要翻译： 组合弹性模量修正微环境微阵列（eMEArray）平台和基于细胞的功能筛选与组合微环境相互作用的方法。 平台和方法允许同时控制分子组成和弹性模量，并结合使用微阵列和微图案技术。 已经使用eMEArrays来显示微环境对药物 - 细胞相互作用的影响，并有助于治疗反应。

公开(公告)号：US08399383B2

公开(公告)日：2013-03-19

申请号：US09849781

申请日：2001-05-04

申请人： Michael Snyder ,  Mark Reed ,  Heng Zhu ,  James Frank Klemic

发明人： Michael Snyder ,  Mark Reed ,  Heng Zhu ,  James Frank Klemic

IPC分类号： C40B40/02 ,  C40B40/10

CPC分类号： C12Q1/485 ,  B01J19/0046 ,  B01J2219/00274 ,  B01J2219/00313 ,  B01J2219/00317 ,  B01J2219/00382 ,  B01J2219/00497 ,  B01J2219/00527 ,  B01J2219/00585 ,  B01J2219/00596 ,  B01J2219/00605 ,  B01J2219/0061 ,  B01J2219/00612 ,  B01J2219/00621 ,  B01J2219/00626 ,  B01J2219/0063 ,  B01J2219/00637 ,  B01J2219/00659 ,  B01J2219/00677 ,  B01J2219/00702 ,  B01J2219/00722 ,  B01J2219/00725 ,  B01L3/5085 ,  B82Y15/00 ,  B82Y30/00 ,  C40B40/06 ,  C40B40/10 ,  C40B60/14 ,  G01N33/5008 ,  G01N33/5041 ,  G01N33/543 ,  G01N33/54313 ,  G01N2500/00

摘要： The present invention relates to protein chips useful for the large-scale study of protein function where the chip contains densely packed reaction wells. The invention also relates to methods of using protein chips to assay simultaneously the presence, amount, and/or function of proteins present in a protein sample or on one protein chip, or to assay the presence, relative specificity, and binding affinity of each probe in a mixture of probes for each of the proteins on the chip. The invention also relates to methods of using the protein chips for high density and small volume chemical reactions. Also, the invention relates to polymers useful as protein chip substrates and methods of making protein chips. The invention further relates to compounds useful for the derivatization of protein chip substrates.

公开(公告)号：US08318508B2

公开(公告)日：2012-11-27

申请号：US12213301

申请日：2008-06-17

申请人： Chad A. Mirkin ,  Ling Huang ,  Rafael Vega ,  Steven Lenhert

发明人： Chad A. Mirkin ,  Ling Huang ,  Rafael Vega ,  Steven Lenhert

IPC分类号： G01N1/00

CPC分类号： C09D11/03 ,  B01J19/0046 ,  B01J2219/00382 ,  B01J2219/00387 ,  B01J2219/00497 ,  B01J2219/00527 ,  B01J2219/00585 ,  B01J2219/00596 ,  B01J2219/00605 ,  B01J2219/00648 ,  B01J2219/00653 ,  B01J2219/00722 ,  B01J2219/00725 ,  B01J2219/00729 ,  B01J2219/00731 ,  B01J2219/00734 ,  B82Y30/00 ,  C09D11/30 ,  C40B50/14 ,  Y10T428/24802 ,  Y10T436/143333 ,  Y10T436/25 ,  Y10T436/2575

摘要： Better patterning methods, including for better methods for forming biomolecular arrays, including a method comprising: providing a tip and a substrate surface, disposing a patterning composition at the end of the tip, depositing at least some of the patterning composition from the tip to the substrate surface to form a deposit disposed on the substrate surface, wherein the patterning composition comprises at least one lipid, optionally at least one solvent, and at least one patterning species different from the lipid and the optional solvent. The lipid can be a phospholipid such as DOPC. The patterning species can be an oligonucleotide or a protein. Microarrays and nanoarrays can be prepared including nanoscale resolution of deposits. The lipid can activate patterning or increase the rate of patterning. Simplified tip preparation can be achieved. Nanoscopic, SPM, and AFM tips can be used.

摘要翻译： 更好的图案化方法，包括用于形成生物分子阵列的更好的方法，包括一种方法，包括：提供尖端和基底表面，在尖端的末端设置图案化组合物，将至少一些图案化组合物从尖端沉积到 衬底表面以形成设置在衬底表面上的沉积物，其中所述图案化组合物包含至少一种脂质，任选的至少一种溶剂和至少一种不同于脂质和任选溶剂的图案化物种。 脂质可以是磷脂如DOPC。 图案化物质可以是寡核苷酸或蛋白质。 可以制备微阵列和纳米阵列，包括沉积物的纳米级分辨率。 脂质可以激活图案化或增加图案化速率。 可以实现简化的尖端准备。 可以使用纳米镜，SPM和AFM提示。

公开(公告)号：US20120231489A1

公开(公告)日：2012-09-13

申请号：US13417650

申请日：2012-03-12

申请人： STEVEN LENHERT

发明人： STEVEN LENHERT

IPC分类号： G01N21/47

CPC分类号： B01J19/0046 ,  B01J2219/00382 ,  B01J2219/00621 ,  B01J2219/00734 ,  G01N21/47 ,  G01N21/4788 ,  G01N21/6458 ,  G01N33/5008 ,  Y10T428/24851

摘要： Described is a method and apparatus for determining the adhesion of an object to an iridescent surface based on the detected scattered light scattered by the interface region for the iridescent surface and the object.

摘要翻译： 描述了一种用于基于由虹彩表面和物体的界面区域散射的检测到的散射光来确定物体与虹彩表面的附着力的方法和装置。