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公开(公告)号：US20220236255A1
公开(公告)日：2022-07-28
申请号：US17622020
申请日：2020-06-26
IPC分类号： G01N33/50 , C12Q1/6872 , C12N5/09 , C12N5/071
摘要： A method of simulating a biological response of a cellular system may include removing at least some DNA-containing material from a vascular plant tissue to produce a vascularized cellulose scaffold, seeding the vascularized cellulose scaffold with cultured biological cells, growing cultured biological cells on the vascularized cellulose scaffold to produce the vascularized biological system, subjecting the vascularized biological system to an external stimulus, and measuring a response of the vascularized biological system. In some embodiments, the removing step comprises submerging the plant tissue in a fluid comprising supercritical CO2, peracetic acid and ethanol.
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公开(公告)号：US20240344022A1
公开(公告)日：2024-10-17
申请号：US18294371
申请日：2022-08-01
发明人： Frederic ZENHAUSERN , Alan NORDQUIST , Baiju THOMAS , Matthew BARRETT , Jerome LACOMBE , Ashlee HARRIS
CPC分类号： C12N5/0062 , C12M25/14 , C12M35/04 , C12N2513/00 , C12N2533/90
摘要： Provided herein are stretch devices and related methods for controlled three-dimensional stretching of scaffolds supported on a stretchable substrate. A stretchable substrate is capable of receiving the three-dimensional scaffold, and a stretchable substrate holder is configured to connect to the stretchable substrate. An actuator is connected to the stretchable substrate holder to exert a cyclic stretch on the stretchable substrate and periodically stretch the three-dimensional scaffold connected to the stretchable substrate. A sample holder is configured to immerse the three-dimensional scaffold in a tissue culture media.
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