公开(公告)号：US12173368B2

公开(公告)日：2024-12-24

申请号：US18298291

申请日：2023-04-10

Applicant: New England Biolabs, Inc.

Inventor： Ivan R. Correa, Jr. ,  Eric Wolf ,  Nan Dai ,  Erbay Yigit ,  Sebastian Grünberg

IPC: C12Q1/6806 ,  C12Q1/6872

Abstract: The present disclosure relates, according to some embodiments, to compositions and analysis of RNA (e.g., dephosphorylated oligoribonucleotides) including, for example, natural and/or synthetic RNAs. A composition may comprise, for example, an endoribonuclease having an amino acid sequence that (i) corresponds to an amino acid sequence of a first species (e.g., Homo sapiens, Escherichia coli, Aspergillus oryzae, Momordica charantia, Pyrococcus furiosus, Cucumis sativus, and Sus scrofa) or (ii) is a non-naturally occurring sequence; and/or an RNA end repair enzyme having an amino acid sequence that (i) corresponds to an amino acid sequence of a species other than the first species (e.g., a bacterial species or a bacteriophage species) or (ii) is a non-naturally occurring sequence.

公开(公告)号：US20240229157A1

公开(公告)日：2024-07-11

申请号：US18558992

申请日：2022-05-03

Applicant: THE REGENTS OF THE UNIVERSITY OF CALIFORNIA ,  WELLCOME SANGER INSTITUTE

Inventor： Nadav AHITUV ,  Ofer YIZHAR-BARNEA ,  Ilias GEORGAKOPOUOS-SOARES ,  Ioannis MOURATIDIS ,  Martin HEMBERG

IPC: C12Q1/6886 ,  C12N9/22 ,  C12N15/10 ,  C12N15/11 ,  C12Q1/6806 ,  C12Q1/6872 ,  G16B20/00 ,  G16H50/20

CPC classification number: C12Q1/6886 ,  C12N9/22 ,  C12N15/1096 ,  C12N15/111 ,  C12Q1/6806 ,  C12Q1/6872 ,  G16B20/00 ,  G16H50/20 ,  C12N2310/20 ,  C12Q2600/156

Abstract: The present disclosure provides methods and compositions for the detection, identification, classification and characterization of cancer in general and cancer types in biological material. Sequences that are not found in the human reference genome or any set of genomic tiled regions, termed nullomers, which can resurface due to mutations, serve as biomarkers and are predictive of cancer. The invention also enables the identification of cancer subtype and the stratification of patients based on sample-specific vulnerabilities guiding treatment choice. For coding nullomers it also covers their use as neoantigens. The algorithms presented hereby can be applied to biological material including biopsy, cell-free DNA samples and RNA samples.

公开(公告)号：US20230235390A1

公开(公告)日：2023-07-27

申请号：US18156739

申请日：2023-01-19

Applicant: Shanghai Blood Center

Inventor： Luyi YE ,  Huijun ZHU ,  Ruishu LI ,  Min FU ,  Ping LU ,  Ziyan ZHU

IPC: C12Q1/6872 ,  C12Q1/6806 ,  C12Q1/6853

CPC classification number: C12Q1/6872 ,  C12Q1/6806 ,  C12Q1/6853 ,  C12Q2600/156 ,  C12Q2600/16

Abstract: The present invention provides a mass spectrometry-based method and a kit for genotyping of platelet and neutrophil antigens and glycoproteins, which are used for genotyping of platelet-specific antigens, platelet CD36 glycoproteins and neutrophil antigens; by designing an optimal primer combination, problems such as homologous sequences and rich GC are overcome, moreover, by improving amplification reaction conditions and using nucleic acid mass spectrometry as a platform, 35 platelet-specific antigen polymorphic sites, 10 CD36 polymorphic sites and 8 neutrophil antigen polymorphic sites can be simultaneously detected in 2 reactions. The present invention has the characteristics of high specificity and sensitivity, and fast and high throughput, and can be used in clinic, scientific research, platelet donor routine screening, etc.

公开(公告)号：US11421347B2

公开(公告)日：2022-08-23

申请号：US16833995

申请日：2020-03-30

Applicant: GENEVILLAGE KFT

Inventor： Csaba Jeney

IPC: C40B30/04 ,  C12N15/10 ,  G01N33/68 ,  C12Q1/6834 ,  C12Q1/6872 ,  G01N30/02 ,  C12Q1/6853 ,  C12Q1/686

Abstract: The present invention relates generally methods and kits for detecting binding interactions, in particular protein-protein interactions, and particularly to high throughput methods for labelling, analysing, detecting and measuring protein-protein interactions.

公开(公告)号：US11339441B2

公开(公告)日：2022-05-24

申请号：US15152996

申请日：2016-05-12

Applicant: The Research Foundation for The State University of New York

Inventor： Rebecca Rose ,  Daniele Fabris

IPC: C12Q1/68 ,  C12Q1/6886 ,  C12Q1/6883 ,  C12Q1/6872 ,  H01J49/00

Abstract: The present invention relates to high-throughput methods comprising direct infusion electrospray ionization mass spectrometry (ESI-MS), multistep tandem mass spectrometry (MSn), consecutive reaction monitoring (CRM), ion mobility spectrometry mass spectrometry (IMS-MS), high-resolution MS, and IMS-MS, for genome-wide (whole cell or tissue) profiling of DNA and RNA nucleotides/nucleosides having a wide variety of variant structural modifications. In particular, these methods are contemplated for providing a specific profile of variant DNA and/or RNA chemically modified nucleic acids (i.e. structures) associated with specific medical conditions. Medical conditions may include, but are not limited to: cancer; including prostate, lung, uterus, larynx, ovary, breast, kidney, and many other types of cancers; specific stages of cancer; bacterial infections; viral infections; genetic and metabolic disorders; and any condition involving changes in DNA and/or RNA structural modifications.

公开(公告)号：US11339434B2

公开(公告)日：2022-05-24

申请号：US15663528

申请日：2017-07-28

Applicant: The Regents of the University of California

Inventor： Markus de Raad ,  Trent R. Northen ,  Curt R. Fischer

IPC: C12Q1/68 ,  C12Q1/25 ,  G01N33/573 ,  C12N15/10 ,  C12P21/06 ,  C12Q1/6872

Abstract: Disclosed herein are methods, systems and compositions for determining substrate specificity of an enzyme. The disclosed methods, systems and compositions can be used for identifying enzymes capable of modifying substrates of interest and/or quantifying enzymatic activity.

公开(公告)号：US20220106637A1

公开(公告)日：2022-04-07

申请号：US17396171

申请日：2021-08-06

Applicant: Agena Bioscience, Inc.

Inventor： Martin BEAULIEU ,  Dirk Johannes VAN DEN BOOM

IPC: C12Q1/6858 ,  C12Q1/6872 ,  C12Q1/6844 ,  C12Q1/6886 ,  C12Q1/6874

Abstract: Provided herein are optimized methods for performing multiplexed detection of a plurality of sequence variations. Also provided are methods for performing multiplexed amplification of target nucleic acid.

公开(公告)号：US11111530B2

公开(公告)日：2021-09-07

申请号：US15863644

申请日：2018-01-05

Applicant: Agena Bioscience, Inc.

Inventor： Martin Beaulieu ,  Dirk Johannes Van Den Boom

IPC: C12P19/34 ,  C12Q1/6858 ,  C12Q1/6872 ,  C12Q1/6844 ,  C12Q1/6886 ,  C12Q1/6874 ,  C12Q1/686

Abstract: Provided herein are optimized methods for performing multiplexed detection of a plurality of sequence variations. Also provided are methods for performing multiplexed amplification of target nucleic acid.

公开(公告)号：US10975424B2

公开(公告)日：2021-04-13

申请号：US16736139

申请日：2020-01-07

Applicant: Omniome, Inc.

Inventor： Corey M. Dambacher ,  Michael Van Nguyen

IPC: C12Q1/68 ,  C12Q1/6853 ,  C12Q1/686 ,  C12Q1/6872 ,  C12Q1/6883 ,  C12Q1/6886 ,  C12Q1/6827

Abstract: A method for identifying target alleles, that includes steps of (a) forming a plurality of stabilized ternary complexes at a plurality of features on an array, wherein the stabilized ternary complexes each has a polymerase, a template nucleic acid having a target allele of a locus, a primer hybridized to the locus, and a next correct nucleotide having a cognate in the locus, wherein either (i) the primer is an allele-specific primer having a 3′ nucleotide that is a cognate nucleotide for the target allele, or (ii) the primer is a locus-specific primer and the next correct nucleotide hybridizes to the target allele; and (b) detecting stabilized ternary complexes at the features, thereby identifying the target alleles.

公开(公告)号：US20200294625A1

公开(公告)日：2020-09-17

申请号：US16825877

申请日：2020-03-20

Applicant: Sequenom, Inc.

Inventor： Sung K. Kim ,  Gregory Hannum ,  Jennifer Geis ,  Cosmin Deciu

IPC: G16B30/00 ,  C12Q1/6816 ,  G16B20/00 ,  C12Q1/6872 ,  C12Q1/6869 ,  G16H50/20

Abstract: Provided herein are methods, processes, systems, machines and apparatuses for non-invasive assessment of genetic variations.