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公开(公告)号：US20240344022A1
公开(公告)日：2024-10-17
申请号：US18294371
申请日：2022-08-01
发明人： Frederic ZENHAUSERN , Alan NORDQUIST , Baiju THOMAS , Matthew BARRETT , Jerome LACOMBE , Ashlee HARRIS
CPC分类号： C12N5/0062 , C12M25/14 , C12M35/04 , C12N2513/00 , C12N2533/90
摘要： Provided herein are stretch devices and related methods for controlled three-dimensional stretching of scaffolds supported on a stretchable substrate. A stretchable substrate is capable of receiving the three-dimensional scaffold, and a stretchable substrate holder is configured to connect to the stretchable substrate. An actuator is connected to the stretchable substrate holder to exert a cyclic stretch on the stretchable substrate and periodically stretch the three-dimensional scaffold connected to the stretchable substrate. A sample holder is configured to immerse the three-dimensional scaffold in a tissue culture media.
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公开(公告)号：US20240277322A1
公开(公告)日：2024-08-22
申请号：US18247596
申请日：2021-10-01
申请人： Arizona Board of Regents on Behalf of the University of Arizona , Wren Laboratories, LLC , Costa Devices Limited
发明人： Frederic ZENHAUSERN , Matthew BARRETT , Baiju THOMAS , Alan NORDQUIST , Jerome LACOMBE , Irvin MODLIN , Mark KIDD
IPC分类号： A61B10/00
CPC分类号： A61B10/0051
摘要： Provided herein are self-collection devices for collecting and/or processing biofluids. The biofluid, such as saliva, is collected in a collection tube. A tube insert in the collection tube is configured to work with a cap having an integrated reservoir volume holding a reagent. Upon connecting the cap to the collection tube, the material in the reservoir volume is effectively introduced to or mixed with the sample in the collection tube. In this manner, there is no risk of user exposure to the reagent and no adverse impact on the ability to reliably mix reagent with sample. The device can then be shipped to a testing facility. Alternatively, a biomarker detection membrane may be used to detect more immediately, without shipping, presence or absence of a material in the sample. This provides for an almost instantaneous read-out that is incorporated into the self-collection device.
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公开(公告)号：US20230347335A1
公开(公告)日：2023-11-02
申请号：US18007456
申请日：2021-07-30
CPC分类号： B01L3/502 , A61L2/24 , A61L2/0023 , A61B10/0051 , A61B5/097 , A01N59/04 , A01P1/00 , A61B10/0096 , B01L2200/185 , B01L2300/1894 , B01L2300/14 , B01L2300/0681 , B01L2300/069 , B01L2200/0678 , B01L2300/042 , A61L2101/02
摘要： Provided herein are portable biocontainment devices for safe and reliable collection of biological samples, including liquid and gas samples. A sample inlet for collecting fluids and/or gases, including oral fluids and expired breath samples, is connected to a container having a climate-controlled container reservoir. One or more sample containers are connected to the sample inlet for collection of samples. The devices are configured for safe transport and handling of samples, while maintaining sample viability, including for relatively long transport periods. Safety components integrated within the device facilitates safe and effective decontamination of contagious samples. In this manner, aborting the sample preservation by decontamination prior to a hazardous release is achieved within the same apparatus.
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公开(公告)号：US20220243160A1
公开(公告)日：2022-08-04
申请号：US17622147
申请日：2020-06-26
摘要： A modular microfluidic device for simulating in vivo conditions of a biological system may include a first perfusion chamber having an inlet and an outlet, a second perfusion chamber having an inlet and an outlet, a well configured to hold one or more 3D structures of cultured biological cells. The well may be in fluid communication with the first and second perfusion chambers. A first porous membrane may be disposed between the first perfusion chamber and the well. A second porous membrane may be disposed between the second perfusion chamber and the well. The well may be configured to facilitate growth of cultured biological cells along all three dimensional axes, thereby providing or ensuring a more representative 3D structure of biological cells compared to conventional monolayer cultures.
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公开(公告)号：US20210079337A1
公开(公告)日：2021-03-18
申请号：US17040427
申请日：2019-03-29
发明人： Frederic ZENHAUSERN , Matthew W. BARRETT , Carla BROOKS , Peng CHEN , Brett M. DUANE , Marie Oceane PARENT , Stanley D. SMITH , Baiju THOMAS , Jianing YANG
摘要： Provided are automated cell culture systems and related in vitro cell culture methods, including for a co-culture of different cell populations having different cell culture conditions. For example, the system may comprise a bioreactor having a plurality of cell culture compartments, the bioreactor having a fluid port fluidly connected to the plurality of cell culture compartments. A pump fluidly connected to the fluid port can provide a cell culture medium to the plurality of culture compartments. A sensor is operably connected to at least one of the plurality of cell culture compartments for measuring at least one cell culture parameter. In this manner, a controller electronically connected the pump and sensor, is configured to automatically adjust a pump flow rate and/or a cell culture gas content to provide a desired steady-state cell culture parameter for facilitating cells interactions and collection of products representative of said interactions.
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公开(公告)号：US20140162262A1
公开(公告)日：2014-06-12
申请号：US14092239
申请日：2013-11-27
CPC分类号： B01L3/502776 , B01F13/0059 , B01F15/0404 , B01L7/52 , B01L2200/0636 , B01L2200/0694 , B01L2300/0816 , B01L2300/0861 , B01L2400/0472 , B01L2400/0487 , G01N33/5304 , Y10T29/49826 , Y10T436/25
摘要： Provided herein are devices and methods for generating microfluidic gradients, including an array of unique microfluidic gradients within an array of microchannels. Fluids within conduits are mixed in an intersection region to generate a mixed flow stream in a source reservoir channel that provides a gradient that varies with axial distance from the intersection region. Microchannels having an inlet connected to the source reservoir channel are configured to provide a microfluidic gradient in the microchannel. An outlet end of the microchannel is connected to a sink reservoir channel. By varying the ratio of fluid flow rates from the fluid conduits, the microchannel gradients are tuned. In this manner, a large number of unique gradients or array of microfluidic gradients is provided, wherein the gradient can be any number of physical or chemical parameters, including concentrations and physical fluid properties.
摘要翻译： 本文提供了用于产生微流体梯度的装置和方法，包括在微通道阵列内的独特微流体梯度的阵列。 管道内的流体在交叉区域中混合，以在源储存器通道中产生混合流动流，其提供随着与交叉区域的轴向距离而变化的梯度。 具有连接到源储存器通道的入口的微通道被配置为在微通道中提供微流体梯度。 微通道的出口端连接到汇水槽通道。 通过改变来自流体导管的流体流速的比率，微通道梯度被调谐。 以这种方式，提供大量独特的梯度或微流体梯度阵列，其中梯度可以是任何数量的物理或化学参数，包括浓度和物理流体性质。
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公开(公告)号：US20220236255A1
公开(公告)日：2022-07-28
申请号：US17622020
申请日：2020-06-26
IPC分类号： G01N33/50 , C12Q1/6872 , C12N5/09 , C12N5/071
摘要： A method of simulating a biological response of a cellular system may include removing at least some DNA-containing material from a vascular plant tissue to produce a vascularized cellulose scaffold, seeding the vascularized cellulose scaffold with cultured biological cells, growing cultured biological cells on the vascularized cellulose scaffold to produce the vascularized biological system, subjecting the vascularized biological system to an external stimulus, and measuring a response of the vascularized biological system. In some embodiments, the removing step comprises submerging the plant tissue in a fluid comprising supercritical CO2, peracetic acid and ethanol.
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公开(公告)号：US20210199651A1
公开(公告)日：2021-07-01
申请号：US17040417
申请日：2019-03-29
发明人： Frederic ZENHAUSERN , Peng CHEN , Jian GU , Jerome LACOMBE
IPC分类号： G01N33/543 , B01L3/00
摘要： Provided are vertical flow detection devices and related methods. The devices may comprise a membrane having a first surface and a second surface with a plurality of porous structures extending between the first and second surfaces to form fluid conduits from a first fluid chamber formed by the first surface and a second fluid chamber formed by the second fluid surface. A capture agent is immobilized on and/or in the membrane. A rigid porous membrane support mechanically supports the membrane and to provide a relatively uniform flow across the membrane. Various gaskets or holder elements are positioned around an outer edge of the membrane to prevent fluid leakage around the membrane. A fluid pump is configured to force a fluid sample flow in a direction from the first fluid chamber to the second fluid chamber.
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公开(公告)号：US20210030347A1
公开(公告)日：2021-02-04
申请号：US17044172
申请日：2019-04-26
发明人： Frederic ZENHAUSERN , Jian GU , Alan NORDQUIST
摘要： Provided is an integrated sample collection device for self-collection and analytical pre-processing of a fluid sample, such as a blood sample from a user. A housing is configured to be held by a user, such as a single hand of a user. A contact-activated penetrating member is disposed at least partially in the housing and configured to penetrate skin. A capillary tube is disposed at least partially in the housing and configured to collect blood released by the penetrating member that penetrates the skin. A vacuum-assisted blood collection container can be fluidically connected to the capillary tube for receiving at least a portion of the collected blood. One or more stabilizing/pre-processing agents are provided for stabilizing/pre-processing collected blood in the capillary tube, the vacuum-assisted blood storage container, or both.
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公开(公告)号：US20220001378A1
公开(公告)日：2022-01-06
申请号：US17293707
申请日：2019-12-05
申请人： Arizona Board of Regents on Behalf of the University of Arizona , The Trustees of Columbia University in the City of New York
发明人： Frederic ZENHAUSERN , Brett DUANE , Jian GU , Alan NORDQUIST , David BRENNER , Mikhail REPIN
IPC分类号： B01L3/00
摘要： Provided herein are methods and related devices for preprocessing a biological sample during transit. The method may comprise the steps of: storing a biological sample in a storage container having walls that defines a storage volume; transporting the storage container with the stored biological sample to a sample processing facility; controlling one or more storage container parameters during the transporting step to initiate preprocessing of the biological sample; wherein the controlling step improves a processing parameter at the sample processing facility.
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