-
公开(公告)号：US06953837B2
公开(公告)日：2005-10-11
申请号：US10003621
申请日：2001-11-02
申请人： Artur Mitterer , Bernhard Fischer , Öyvind L. Schönberger , Kathrin Thomas-Urban , Friedrich Dorner , Johann Eibl
发明人： Artur Mitterer , Bernhard Fischer , Öyvind L. Schönberger , Kathrin Thomas-Urban , Friedrich Dorner , Johann Eibl
CPC分类号： A61K38/37 , C07K14/755
摘要： There is disclosed a method of recovering factor VIII/vWF-complex which is characterized in that factor VIII/vWF-complex from a protein solution is bound to a cation exchanger and is recovered by step-wise elution of factor VIII/vWF-complex, which particularly contains high-molecular vWF multimers, as well as a factor VIII/vWF-complex obtainable by means of cation exchange chromatography.
摘要翻译： 公开了一种回收因子VIII / vWF-复合物的方法，其特征在于将来自蛋白质溶液的因子VIII / vWF-复合物结合到阳离子交换剂并通过逐步洗脱因子VIII / vWF-复合物来回收， 其特别包含高分子vWF多聚体，以及通过阳离子交换层析可获得的因子VIII / vWF-络合物。
-
2.发明授权Method for purifying factor VIII/vWF company by cation-exchange chromatography 有权通过阳离子交换色谱法纯化因子VIII / VWF-复合物的方法公开(公告)号：US06831159B1
公开(公告)日：2004-12-14
申请号：US09367459
申请日：2000-05-08
申请人： Artur Mitterer , Bernhard Fischer , Öyvind L. Schönberger , Kathrin Thomas-Urban , Friedrich Dorner , Johann Eibl
发明人： Artur Mitterer , Bernhard Fischer , Öyvind L. Schönberger , Kathrin Thomas-Urban , Friedrich Dorner , Johann Eibl
IPC分类号： A23J100
CPC分类号： A61K38/37 , C07K14/755
摘要： Methods for recovering factor VIII/vWF-complex that involve binding factor VIII/vWF-complex from a protein solution to a cation exchanger and recovering factor VIII/vWF-complex by step-wise elution are disclosed. The recovered complex contains high-molecular vWF multimers. Compositions containing factor VIII/vWF-complex as purified by cation exchange chromatography are also provided.
摘要翻译： 公开了将蛋白质溶液与阳离子交换剂结合的因子VIII / vWF-复合物的因子VIII / vWF-复合物的回收方法，通过逐步洗脱回收因子VIII / vWF-复合物。 回收的复合物含有高分子vWF多聚体。 还提供了通过阳离子交换层析纯化的含有因子VIII / vWF-络合物的组合物。
-
3.发明授权公开(公告)号：US06465624B1
公开(公告)日：2002-10-15
申请号：US09367460
申请日：1999-10-21
申请人： Bernhard Fischer , Öyvind L. Schönberger , Artur Mitterer , Christian Fiedler , Friedrich Dorner , Johann Eibl
发明人： Bernhard Fischer , Öyvind L. Schönberger , Artur Mitterer , Christian Fiedler , Friedrich Dorner , Johann Eibl
IPC分类号： A23J100
CPC分类号： C07K14/755 , A61K38/00
摘要： Disclosed are a method of recovering vWF in which vWF at a low salt concentration is bound to a cation exchanger and vWF having a high specific activity is recovered by fractionated elution, as well as a preparation having purified vWF obtainable by this method.
摘要翻译： 公开了一种回收vWF的方法，其中低盐浓度的vWF与阳离子交换剂结合，并且通过分级洗脱回收具有高比活性的vWF，以及通过该方法可获得纯化的vWF的制剂。
-
公开(公告)号：US07648958B2
公开(公告)日：2010-01-19
申请号：US10789562
申请日：2004-02-27
申请人： Artur Mitterer , Bernhard Fischer , Oyvind L. Schönberger , Kathrin Thomas-Urban , Friedrich Dorner , Johann Eibl
发明人： Artur Mitterer , Bernhard Fischer , Oyvind L. Schönberger , Kathrin Thomas-Urban , Friedrich Dorner , Johann Eibl
IPC分类号： A01N37/18
CPC分类号： A61K38/37 , C07K14/755
摘要： There is disclosed a method of recovering factor VIII/vWF-complex which is characterized in that factor VIII/vWF-complex from a protein solution is bound to a cation exchanger and is recovered by step-wise elution of factor VIII/vWF-complex, which particularly contains high-molecular vWF multimers, as well as a factor VIII/vWF-complex obtainable by means of cation exchange chromatography.
摘要翻译： 公开了一种回收因子VIII / vWF-复合物的方法，其特征在于将来自蛋白质溶液的因子VIII / vWF-复合物结合到阳离子交换剂并通过逐步洗脱因子VIII / vWF-复合物来回收， 其特别包含高分子vWF多聚体，以及通过阳离子交换层析可获得的因子VIII / vWF-络合物。
-
5.发明申请Method for purifying factor VIII/vWF-complex by means of cation exchange chromatography 有权通过阳离子交换色谱法纯化因子VIII / vWF-复合物的方法公开(公告)号：US20050239171A1
公开(公告)日：2005-10-27
申请号：US10789562
申请日：2004-02-27
申请人： Artur Mitterer , Bernhard Fischer , Oyvind Schonberger , Kathrin Thomas-Urban , Friedrich Dorner , Johann Eibl
发明人： Artur Mitterer , Bernhard Fischer , Oyvind Schonberger , Kathrin Thomas-Urban , Friedrich Dorner , Johann Eibl
CPC分类号： A61K38/37 , C07K14/755
摘要： There is disclosed a method of recovering factor VIII/vWF-complex which is characterized in that factor VIII/vWF-complex from a protein solution is bound to a cation exchanger and is recovered by step-wise elution of factor VIII/vWF-complex , which particularly contains high-molecular vWF multimers, as well as a factor VIII/vWF-complex obtainable by means of cation exchange chromatography.
摘要翻译： 公开了一种回收因子VIII / vWF-复合物的方法，其特征在于将来自蛋白质溶液的因子VIII / vWF-复合物结合到阳离子交换剂并通过逐步洗脱因子VIII / vWF-复合物来回收， 其特别包含高分子vWF多聚体，以及通过阳离子交换层析可获得的因子VIII / vWF-络合物。
-
公开(公告)号：US20050209154A1
公开(公告)日：2005-09-22
申请号：US11132326
申请日：2005-05-19
IPC分类号： A61K38/43 , A61K31/00 , A61K38/37 , A61P7/00 , A61P7/04 , C07K1/18 , C07K1/22 , C07K14/745 , C07K14/755
CPC分类号： C07K14/755 , Y10S514/834 , Y10T436/25375
摘要： There are disclosed a stable factor VIII/vWF-complex, particularly comprising high-molecular vWF multimers, being free from low-molecular vWF molecules and from proteolytic vWF degradation products, as well as a method of producing this complex.
摘要翻译： 公开了稳定的因子VIII / vWF-复合物，特别是包含不含低分子vWF分子和蛋白水解vWF降解产物的高分子vWF多聚体，以及生产该复合物的方法。
-
7.发明授权Method of chromatographically purifying or fractionating, respectively, von Willebrand factor from a VWF-containing starting material 失效分别从含VWF的起始材料中色谱纯化或分馏von Willebrand因子的方法公开(公告)号：US06414125B1
公开(公告)日：2002-07-02
申请号：US09355865
申请日：1999-10-21
申请人： Juergen Siekmann , Peter Turecek , Hans-Peter Schwarz , Johann Eibl , Bernhard Fischer , Artur Mitterer , Friedrich Dorner
发明人： Juergen Siekmann , Peter Turecek , Hans-Peter Schwarz , Johann Eibl , Bernhard Fischer , Artur Mitterer , Friedrich Dorner
IPC分类号： A23J100
CPC分类号： C07K14/755
摘要： Disclosed is a method of chromatographically purifying or fractionating, respectively, von Willebrand factor (vWF) from a vWF-containing starting material, comprising the following steps: adsorbing the vWF from the starting material on avid collagen immobilized on a carrier, separating the non-adsorbed portion and, optionally, washing the carrier, eluting the vWF from immobilized collagen, and recovering the purified vWF, as well as a pharmaceutical preparation comprising biologically active vWF which is bound to collagen in a stable manner.
摘要翻译： 公开了一种从含有vWF的原料分别纯化或分馏von Willebrand因子（vWF）的方法，其包括以下步骤：将起始材料的vWF吸附在固定在载体上的avid胶原上， 吸附部分和任选地洗涤载体，从固定化胶原洗脱vWF，并回收纯化的vWF，以及包含以稳定方式与胶原结合的生物活性vWF的药物制剂。
-
公开(公告)号：US5877152A
公开(公告)日：1999-03-02
申请号：US898131
申请日：1997-07-22
申请人： Bernhard Fischer , Artur Mitterer , Friedrich Dorner , Hans-Peter Schwarz , Peter Turecek , Johann Eibl , Falko-Guenter Falkner , Uwe Schlokat , Wolfgang Mundt , Manfred Reiter , Renate Den-Bouwmeester
发明人： Bernhard Fischer , Artur Mitterer , Friedrich Dorner , Hans-Peter Schwarz , Peter Turecek , Johann Eibl , Falko-Guenter Falkner , Uwe Schlokat , Wolfgang Mundt , Manfred Reiter , Renate Den-Bouwmeester
IPC分类号： C12N15/09 , A61K35/14 , A61K38/00 , A61K38/43 , A61P7/04 , C07K1/18 , C07K1/22 , C07K14/47 , C07K14/755 , C12P21/00 , A61K38/17
CPC分类号： C07K14/755 , A61K38/00
摘要： The invention relates to a method for isolation of highly pure von Willebrand Factor in which recombinant von Willebrand Factor (rvWF) is chromatographically purified by anion exchange chromatography on an anion exchanger of the quaternary amino type in a buffer solution comprising buffer substances and optionally salt.The buffer solutions are preferably free of stabilizers, amino acids and other additives. According to this method, highly pure recombinant vWF can be obtained, which is free from blood plasma proteins, especially free from Factor VIII, and is physiologically active.Further, the invention relates to a pharmaceutical preparation that contains rvWF, which is comprised of multimers with a high structural integrity
摘要翻译： 本发明涉及一种用于分离高纯度血管性血友病因子的方法，其中重组血管性血友病因子（rvWF）通过阴离子交换色谱在季氨基型阴离子交换剂上在包含缓冲物质和任选盐的缓冲溶液中进行色谱纯化。 缓冲溶液优选不含稳定剂，氨基酸和其它添加剂。 根据该方法，可以获得高纯度的重组vWF，其不含血浆蛋白质，特别是不含因子VIII，且具有生理活性。 此外，本发明涉及含有rvWF的药物制剂，其由具有高结构完整性的多聚体组成
-
公开(公告)号：US06228613B1
公开(公告)日：2001-05-08
申请号：US09142768
申请日：1998-11-06
IPC分类号： C12P2106
CPC分类号： C07K14/755 , Y10S514/834 , Y10T436/25375
摘要： There are disclosed a stable factor VIII/vWF-complex, particularly comprising high-molecular vWF multimers, being free from low-molecular vWF molecules and from proteolytic vWF degradation products, as well as a method of producing this complex.
摘要翻译： 公开了稳定的因子VIII / vWF-复合物，特别是包含不含低分子vWF分子和蛋白水解vWF降解产物的高分子vWF多聚体，以及生产该复合物的方法。
-
公开(公告)号：US5854403A
公开(公告)日：1998-12-29
申请号：US653298
申请日：1996-05-24
申请人： Bernhard Fischer , Artur Mitterer , Friedrich Dorner , Hans-Peter Schwarz , Peter Turecek , Johann Eibl , Falko-Guenter Falkner , Uwe Schlokat , Wolfgang Mundt , Manfred Reiter , Renate Den-Bouwmeester
发明人： Bernhard Fischer , Artur Mitterer , Friedrich Dorner , Hans-Peter Schwarz , Peter Turecek , Johann Eibl , Falko-Guenter Falkner , Uwe Schlokat , Wolfgang Mundt , Manfred Reiter , Renate Den-Bouwmeester
IPC分类号： C12N15/09 , A61K35/14 , A61K38/00 , A61K38/43 , A61P7/04 , C07K1/18 , C07K1/22 , C07K14/47 , C07K14/755 , C12P21/00 , C07K1/14
CPC分类号： C07K14/755 , A61K38/00
摘要： The invention relates to a method for isolation of highly pure von Willebrand Factor in which recombinant von Willebrand Factor (rvWF) is chromatographically purified by anion exchange chromatography on an anion exchanger of the quaternary amino type in a buffer solution comprising buffer substances and optionally salt. The buffer solutions are preferably free of stabilizers, amino acids and other additives. According to this method, highly pure recombinant rvWF can be obtained, which is free from blood plasma proteins, especially free from Factor VIII, and is physiologically active. Further, the invention relates to a pharmaceutical preparation that contains rvWF, which comprises mulitimers with a high structural integrity.
摘要翻译： 本发明涉及一种用于分离高纯度血管性血友病因子的方法，其中重组血管性血友病因子（rvWF）通过阴离子交换色谱在季氨基型阴离子交换剂上在包含缓冲物质和任选盐的缓冲溶液中进行色谱纯化。 缓冲溶液优选不含稳定剂，氨基酸和其它添加剂。 根据该方法，可以获得高纯度的重组rvWF，其不含血浆蛋白质，特别是不含因子VIII，且具有生理活性。 此外，本发明涉及含有rvWF的药物制剂，其包含具有高结构完整性的多组分。
-
-
-
-
-
-
-
-
-
搜索结果
59 条记录 (耗时 0.028 秒)
