公开(公告)号：US20200046767A1

公开(公告)日：2020-02-13

申请号：US16485488

申请日：2018-02-12

申请人： Assistance Publique - Hopitaux de Paris ,  Fondation Imagine - Institut des Maladies Génétiques ,  Université Paris Descartes ,  Institut National de la Santé et de la Recherche Médicale

发明人： Isabelle André ,  Marina Cavazzana ,  Kuiying Ma ,  John Tchen

IPC分类号： A61K35/17 ,  C12N5/0783

摘要： The invention relates to an in vitro method to generate T cell progenitors, comprising the step of culturing CD34+ cells in a medium containing TNF-alpha and/or an antagonist of the Aryl hydro-carbon/Dioxin receptor, in particular StemRegenin 1 (SR1), in presence of a Notch ligand and optionally a fibronectin fragment.

公开(公告)号：US11638723B2

公开(公告)日：2023-05-02

申请号：US17173632

申请日：2021-02-11

申请人： Assistance Publique—Hopitaux de Paris ,  Fondation Imagine—Institut des Maladies Génétiques ,  Université Paris Cité ,  Institut National de la Santé et de la Recherche Médicale (INSERM)

发明人： Isabelle André ,  Marina Cavazzana ,  Kuiying Ma ,  John Tchen ,  Tayebeh-Shabi Soheili ,  Ranjita Devi Moirangthem

IPC分类号： C12N5/00 ,  C12N15/63 ,  C12N5/02 ,  A61K35/17 ,  C12N5/0783 ,  A61K31/70

摘要： The invention relates to an in vitro method to generate T cell progenitors, comprising the step of culturing CD34+ cells in a medium containing TNF-alpha and/or an antagonist of the Aryl hydrocarbon/Dioxin receptor, in particular StemRegenin 1 (SR1), in presence of a Notch ligand and optionally a fibronectin fragment.

公开(公告)号：US20210169935A1

公开(公告)日：2021-06-10

申请号：US17173668

申请日：2021-02-11

申请人： Assistance Publique - Hopitaux de Paris ,  Fondation Imagine - Institut des Maladies Génétiques ,  Université de Paris ,  Institut National de la Santé et de la Recherche Médicale (INSERM)

发明人： Isabelle André ,  Marina Cavazzana ,  Kuiying Ma ,  John Tchen

IPC分类号： A61K35/17 ,  C12N5/0783

摘要： The invention relates to an in vitro method to generate T cell progenitors, comprising the step of culturing CD34+ cells in a medium containing TNF-alpha and/or an antagonist of the Aryl hydro-carbon/Dioxin receptor, in particular StemRegenin 1 (SR1), in presence of a Notch ligand and optionally a fibronectin fragment.

公开(公告)号：US11642376B2

公开(公告)日：2023-05-09

申请号：US17173668

申请日：2021-02-11

申请人： Assistance Publique—Hopitaux de Paris ,  Fondation Imagine—Institut des Maladies Génétiques ,  Université Paris Cité ,  Institut National de la Santé et de la Recherche Médicale (INSERM)

发明人： Isabelle André ,  Marina Cavazzana ,  Kuiying Ma ,  John Tchen ,  Tayebeh-Shabi Soheili ,  Ranjita Devi Moirangthem

IPC分类号： A61K48/00 ,  C12N5/00 ,  A61K35/17 ,  C12N5/0783 ,  A01K67/00

CPC分类号： A61K35/17 ,  C12N5/0636 ,  C12N2501/125 ,  C12N2501/145 ,  C12N2501/2307 ,  C12N2501/25 ,  C12N2501/26 ,  C12N2501/42 ,  C12N2506/11 ,  C12N2533/52

摘要： The invention relates to an in vitro method to generate T cell progenitors, comprising the step of culturing CD34+ cells in a medium containing TNF-alpha and/or an antagonist of the Aryl hydro-carbon/Dioxin receptor, in particular StemRegenin 1 (SR1), in presence of a Notch ligand and optionally a fibronectin fragment.

公开(公告)号：US11426430B2

公开(公告)日：2022-08-30

申请号：US17010208

申请日：2020-09-02

申请人： Assistance Publique—Hopitaux de Paris ,  Fondation Imagine—Institut des Maladies Génétiques ,  Université de Paris Cité ,  Institut National de la Santé et de la Recherche Médicale (INSERM)

发明人： Isabelle André ,  Marina Cavazzana ,  Kuiying Ma ,  John Tchen ,  Tayebeh-Shabi Soheili ,  Ranjita Devi Moirangthem

IPC分类号： C12N5/00 ,  C12N5/02 ,  A61K35/17 ,  C12N5/0783 ,  A61K48/00

摘要： The invention relates to an in vitro method to generate T cell progenitors, comprising the step of culturing CD34+ cells in a medium containing TNF-alpha and/or an antagonist of the Aryl hydro-carbon/Dioxin receptor, in particular StemRegenin 1 (SR1), in presence of a Notch ligand and optionally a fibronectin fragment.

公开(公告)号：US20210169934A1

公开(公告)日：2021-06-10

申请号：US17173632

申请日：2021-02-11

申请人： Assistance Publique - Hopitaux de Paris ,  Fondation Imagine - Institut des Maladies Génétiques ,  Université de Paris ,  Institut National de la Santé et de la Recherche Médicale (INSERM)

发明人： Isabelle André ,  Marina Cavazzana ,  Kuiying Ma ,  John Tchen

IPC分类号： A61K35/17 ,  C12N5/0783

摘要： The invention relates to an in vitro method to generate T cell progenitors, comprising the step of culturing CD34+ cells in a medium containing TNF-alpha and/or an antagonist of the Aryl hydrocarbon/Dioxin receptor, in particular StemRegenin 1 (SR1), in presence of a Notch ligand and optionally a fibronectin fragment.

公开(公告)号：US12071635B2

公开(公告)日：2024-08-27

申请号：US15516731

申请日：2015-10-05

申请人： Assistance Publique-Hopitaux de Paris ,  Fondation Imagine-Institut Des Maladies Genetiques ,  Universtie Paris Descartes ,  Institut National de la Sante et de la Recherche Medicale (INSERM)

发明人： Marina Cavazzana ,  Isabelle André ,  Chantal Lagresle-Peyrou ,  Salima Hacein-Bey-Abina ,  Christian Reimann ,  Corinne De Chappedelaine ,  Emmanuelle Six

IPC分类号： C12N5/00 ,  C12N5/0783

CPC分类号： C12N5/0636 ,  C12N2501/42 ,  C12N2502/1114 ,  C12N2506/11 ,  C12N2533/50 ,  C12N2533/52

摘要： The invention relates to the field of cell therapy and to an in vitro method for generating T-cell progenitors, comprising the step of exposing CD34+ cells in a medium containing a Notch ligand, a soluble domain of the Delta-like ligand 4, joined to an Fc region of a protein IgG, in the presence of a fragment of fibronectin comprising the motifs RGDS and CS-1 and a heparin-binding domain.