公开(公告)号：US6130055A

公开(公告)日：2000-10-10

申请号：US135915

申请日：1998-08-18

申请人： Atsuko Nanbu ,  Satoshi Fukunaga

发明人： Atsuko Nanbu ,  Satoshi Fukunaga

IPC分类号： G01N33/15 ,  C12Q1/37 ,  C12Q1/34

CPC分类号： C12Q1/37 ,  C12Q2337/12 ,  G01N2333/811 ,  G01N2333/8114 ,  G01N2333/976 ,  Y10S435/962

摘要： A method for measuring the concentration or the activity of UTI quickly and easily at high sensitivity. A urine sample, a buffer solution, a trypsin solution and a substrate solution are mixed and the trypsin activity is then measured. Thus, the UTI concentration in the urine sample is determined. In this case, a substrate solution having only L-BAPNA is used as the substrate, and the surfactant is mixed in at least one selected from the buffer solution and the enzyme solution. The mixing ratio of the surfactant is about 1 wt. % in the entire enzyme reaction solution. Examples of the surfactant include polyoxyethylene (40) octylphenylether, polyoxyethylene (10) octylphenylether, 3-[(3-cholamido propyl)dimethylammonio]-propanesulfonic acid, 3-[(3-cholamido propyl)dimethylammonio]-2-hydroxypropanesulfonic acid, and polyoxyethylene sorbitan monolaurate. As shown in FIG. 1, the sensitivity improves when using L-BAPNA.

摘要翻译： 用于以高灵敏度快速，容易地测量UTI的浓度或活性的方法。 将尿样，缓冲溶液，胰蛋白酶溶液和底物溶液混合，然后测定胰蛋白酶活性。 因此，测定尿样中的UTI浓度。 在这种情况下，使用仅具有L-BAPNA的底物溶液作为底物，并且将表面活性剂混合在选自缓冲溶液和酶溶液中的至少一种。 表面活性剂的混合比为约1wt。 ％在整个酶反应溶液中。 表面活性剂的实例包括聚氧乙烯（40）辛基苯基醚，聚氧乙烯（10）辛基苯基醚，3 - [（3-胆酰胺基丙基）二甲基氨基] - 丙磺酸，3 - [（3-胆酰胺基丙基）二甲基氨基] -2-羟基丙磺酸和 聚氧乙烯脱水山梨醇单月桂酸酯。 如图所示。 1，使用L-BAPNA时灵敏度提高。

公开(公告)号：US5856117A

公开(公告)日：1999-01-05

申请号：US879962

申请日：1997-06-20

申请人： Harumi Uenoyama ,  Kyouichi Ohshiro ,  Atsuko Nanbu ,  Satoshi Fukunaga

发明人： Harumi Uenoyama ,  Kyouichi Ohshiro ,  Atsuko Nanbu ,  Satoshi Fukunaga

IPC分类号： C12Q1/37 ,  C12Q1/00 ,  G01N33/53

CPC分类号： C12Q1/37 ,  C12Q2337/12 ,  G01N2333/81 ,  G01N2333/811 ,  G01N2333/976 ,  Y10S435/963 ,  Y10S435/975

摘要： This invention provides a method for measuring the concentration of urinary trypsin inhibitors which is excellent in precision and reproducibility, whose operation is simple, and in which a possibility of damaging a plastic cell is eliminated. The method for measuring the concentration of urinary trypsin inhibitors comprises mixing an urine sample, a protease solution containing trypsin, and a buffer solution, adding a substrate solution to the mixture to cause the enzyme reaction, and measuring the activity of the enzyme, wherein the buffer solution is prepared so that it contains at least 0.15 .mu.mol calcium per 1 .mu.g of the trypsin but no more than 100 .mu.mol calcium per 1 ml of the urine sample in the reaction mixture, and wherein the substrate solution is prepared by dissolving the substrate in an organic solvent and diluting the mixture solution with aqueous medium, wherein at least one of an amphoteric surfactant and a nonionic surfactant is added to at least one of the organic solvent and the aqueous medium.

摘要翻译： 本发明提供了一种测定尿胰蛋白酶抑制剂浓度的方法，其精度和重复性优异，操作简单，消除了塑料细胞损伤的可能性。 用于测定尿胰蛋白酶抑制剂浓度的方法包括混合尿液样品，含有胰蛋白酶的蛋白酶溶液和缓冲溶液，向混合物中加入底物溶液以引起酶反应，并测量酶的活性，其中 制备缓冲液，使其每1ml胰蛋白酶含有至少0.15μmol钙，而在反应混合物中每1ml尿液含有不超过100μmol钙，其中底物溶液通过溶解 将所述底物置于有机溶剂中并用水性介质稀释所述混合溶液，其中至少一种两性表面活性剂和非离子表面活性剂加入至少一种有机溶剂和水性介质中。

公开(公告)号：US08993344B2

公开(公告)日：2015-03-31

申请号：US13258374

申请日：2010-07-16

申请人： Hironori Hasegawa ,  Kyouichi Ohshiro ,  Satoshi Fukunaga

发明人： Hironori Hasegawa ,  Kyouichi Ohshiro ,  Satoshi Fukunaga

IPC分类号： G01N33/53 ,  G01N33/557 ,  G01N33/558

CPC分类号： G01N33/557 ,  G01N33/5306 ,  G01N33/558

摘要： Provided is a prozone phenomenon detecting method, by which generation of a prozone phenomenon can be detected even when a conventional specimen analysis tool is used, and examinations using an immunochromatography method and the like can be performed efficiently.

摘要翻译： 提供了一种前区现象检测方法，即使使用常规的样本分析工具也能够检测到前区现象的产生，并且可以有效地执行使用免疫色谱法等的检查。

公开(公告)号：US07054759B2

公开(公告)日：2006-05-30

申请号：US10500034

申请日：2002-12-25

申请人： Satoshi Fukunaga ,  Masato Nakayama

发明人： Satoshi Fukunaga ,  Masato Nakayama

IPC分类号： G06N31/00

CPC分类号： G01N21/272 ,  G01N21/274 ,  G01N21/75 ,  G01N21/83 ,  G01N35/00693

摘要： A concentration measuring method includes selecting a calibration curve optimum for computing the concentration of a measurement target substance from a plurality of calibration curves based on an output from a reaction system containing the target substance and a reactant capable of reacting with the target substance, and computing the concentration of the target substance based on the optimum calibration curve and the output. Each of the calibration curves is prepared based on a plurality of outputs generated upon lapse of a same reaction time from a plurality of standard reaction systems each containing a standard reagent of a known different concentration and the reactant. The plurality of calibration curves differ from each other in reaction time based on which the calibration curves are prepared.

摘要翻译： 一种浓度测定方法，其特征在于，根据来自含有所述目标物质的反应体系和能够与所述目标物质反应的反应物的输出，从多个校正曲线中选择最适于计算测定对象物质的浓度的校正曲线， 基于最佳校准曲线和输出的目标物质的浓度。 基于经过相同反应时间后产生的多个输出，每个标准反应体系都含有已知不同浓度的标准试剂和反应物，每个标准反应体系都准备了每个校正曲线。 在准备校准曲线的基础上，反应时间中多个校正曲线彼此不同。

公开(公告)号：US20050107956A1

公开(公告)日：2005-05-19

申请号：US10500034

申请日：2002-12-25

申请人： Satoshi Fukunaga ,  Masato Nakayama

发明人： Satoshi Fukunaga ,  Masato Nakayama

IPC分类号： G01N21/27 ,  G01N21/75 ,  G06F19/00 ,  G01N31/00 ,  G01N33/48 ,  G01N33/50

CPC分类号： G01N21/272 ,  G01N21/274 ,  G01N21/75 ,  G01N21/83 ,  G01N35/00693

摘要： The present invention relates to a concentration measuring method which includes selecting a calibration curve optimum for computing the concentration of a measurement target substance from a plurality of calibration curves based on an output from a reaction system containing the target substance and a reactant capable of reacting with the target substance, and computing the concentration of the target substance based on the optimum calibration curve and the output. Each of the calibration curves is prepared based on a plurality of outputs generated upon lapse of a same reaction time from a plurality of standard reaction systems each containing a standard reagent of a known different concentration and the reactant. The plurality of calibration curves differ from each other in reaction time based on which the calibration curves are prepared. For instance, the plurality of calibration curves include a first calibration curve prepared based on an output measured in an initial stage of the reaction between a standard substance of a known concentration and the reactant, and a second calibration curve prepared based on an output measured after the output as the base for the preparation of the first calibration curve is measured.

摘要翻译： 浓度测定方法本发明涉及一种浓度测定方法，其特征在于，包括从多个校准曲线中选出最适于计算测定对象物质浓度的校正曲线，该校正曲线基于含有目标物质的反应体系和能够与 目标物质，并根据最佳校准曲线和输出计算目标物质的浓度。 基于经过相同反应时间后产生的多个输出，每个标准反应体系都含有已知不同浓度的标准试剂和反应物，每个标准反应体系都准备了每个校正曲线。 在准备校准曲线的基础上，反应时间中多个校正曲线彼此不同。 例如，多个校准曲线包括基于在已知浓度的标准物质与反应物之间的反应的初始阶段测量的输出制备的第一校准曲线和基于后面测量的输出制备的第二校准曲线 测量作为制备第一校准曲线的基准的输出。

公开(公告)号：US20120015450A1

公开(公告)日：2012-01-19

申请号：US13258374

申请日：2010-07-16

申请人： Hironori Hasegawa ,  Kyouichi Ohshiro ,  Satoshi Fukunaga

发明人： Hironori Hasegawa ,  Kyouichi Ohshiro ,  Satoshi Fukunaga

IPC分类号： G01N33/53 ,  B01J19/00

CPC分类号： G01N33/557 ,  G01N33/5306 ,  G01N33/558

摘要： Provided is a prozone phenomenon detecting method, by which generation of a prozone phenomenon can be detected even when a conventional specimen analysis tool is used, and examinations using an immunochromatography method and the like can be performed efficiently. In the method, a specimen analysis tool containing substances that specifically bind to a target component contained in a sample is used. The specimen analysis tool is obtained by arranging a sample supplying portion, a reagent portion, and a detection portion on the porous base material from upstream to downstream in a sample moving direction in this order. The reagent portion contains a labeled substance that specifically binds to the target component. The detection portion contains an immobilized substance that specifically binds to the target component. The target component is detected by detecting a complex of the target component, the labeled substance, and the immobilized substance through detection of a label of the labeled substance in the detection portion. The method includes at least one of the following processes A and B: the process A: a process in which detection results obtained in the detection portion are plotted along the sample moving direction, and generation of a prozone phenomenon is detected on the basis of a position of a peak in plots thus obtained; and the process B: a process in which the label is detected at two or more different time points in the detection portion, and generation of a prozone phenomenon is detected on the basis of a magnitude relationship between two or more detection results thus obtained.

摘要翻译： 提供了一种前区现象检测方法，即使使用常规的样本分析工具也能够检测到前区现象的产生，并且可以有效地执行使用免疫色谱法等的检查。 在该方法中，使用含有与试样中含有的靶成分特异结合的物质的试样分析工具。 样品分析工具通过在样品移动方向的上游到下游依次布置多孔基材上的样品供应部分，试剂部分和检测部分而获得。 试剂部分含有与目标成分特异性结合的标记物质。 检测部分含有特异性结合目标成分的固定化物质。 通过检测检测部中的标记物质的标签，检测目标成分，标记物质和固定化物质的复合物来检测目标成分。 该方法包括以下过程A和B中的至少一个：处理A：沿着样品移动方向绘制在检测部分中获得的检测结果的过程，并且基于 如此获得的图中峰的位置; 过程B：在检测部分中的两个或更多个不同时间点检测标签的过程，并且基于由此获得的两个或更多个检测结果之间的大小关系来检测前区现象的产生。