公开(公告)号：US5856117A

公开(公告)日：1999-01-05

申请号：US879962

申请日：1997-06-20

申请人： Harumi Uenoyama ,  Kyouichi Ohshiro ,  Atsuko Nanbu ,  Satoshi Fukunaga

发明人： Harumi Uenoyama ,  Kyouichi Ohshiro ,  Atsuko Nanbu ,  Satoshi Fukunaga

IPC分类号： C12Q1/37 ,  C12Q1/00 ,  G01N33/53

CPC分类号： C12Q1/37 ,  C12Q2337/12 ,  G01N2333/81 ,  G01N2333/811 ,  G01N2333/976 ,  Y10S435/963 ,  Y10S435/975

摘要： This invention provides a method for measuring the concentration of urinary trypsin inhibitors which is excellent in precision and reproducibility, whose operation is simple, and in which a possibility of damaging a plastic cell is eliminated. The method for measuring the concentration of urinary trypsin inhibitors comprises mixing an urine sample, a protease solution containing trypsin, and a buffer solution, adding a substrate solution to the mixture to cause the enzyme reaction, and measuring the activity of the enzyme, wherein the buffer solution is prepared so that it contains at least 0.15 .mu.mol calcium per 1 .mu.g of the trypsin but no more than 100 .mu.mol calcium per 1 ml of the urine sample in the reaction mixture, and wherein the substrate solution is prepared by dissolving the substrate in an organic solvent and diluting the mixture solution with aqueous medium, wherein at least one of an amphoteric surfactant and a nonionic surfactant is added to at least one of the organic solvent and the aqueous medium.

摘要翻译： 本发明提供了一种测定尿胰蛋白酶抑制剂浓度的方法，其精度和重复性优异，操作简单，消除了塑料细胞损伤的可能性。 用于测定尿胰蛋白酶抑制剂浓度的方法包括混合尿液样品，含有胰蛋白酶的蛋白酶溶液和缓冲溶液，向混合物中加入底物溶液以引起酶反应，并测量酶的活性，其中 制备缓冲液，使其每1ml胰蛋白酶含有至少0.15μmol钙，而在反应混合物中每1ml尿液含有不超过100μmol钙，其中底物溶液通过溶解 将所述底物置于有机溶剂中并用水性介质稀释所述混合溶液，其中至少一种两性表面活性剂和非离子表面活性剂加入至少一种有机溶剂和水性介质中。

公开(公告)号：US08993344B2

公开(公告)日：2015-03-31

申请号：US13258374

申请日：2010-07-16

申请人： Hironori Hasegawa ,  Kyouichi Ohshiro ,  Satoshi Fukunaga

发明人： Hironori Hasegawa ,  Kyouichi Ohshiro ,  Satoshi Fukunaga

IPC分类号： G01N33/53 ,  G01N33/557 ,  G01N33/558

CPC分类号： G01N33/557 ,  G01N33/5306 ,  G01N33/558

摘要： Provided is a prozone phenomenon detecting method, by which generation of a prozone phenomenon can be detected even when a conventional specimen analysis tool is used, and examinations using an immunochromatography method and the like can be performed efficiently.

摘要翻译： 提供了一种前区现象检测方法，即使使用常规的样本分析工具也能够检测到前区现象的产生，并且可以有效地执行使用免疫色谱法等的检查。

公开(公告)号：US20120015450A1

公开(公告)日：2012-01-19

申请号：US13258374

申请日：2010-07-16

申请人： Hironori Hasegawa ,  Kyouichi Ohshiro ,  Satoshi Fukunaga

发明人： Hironori Hasegawa ,  Kyouichi Ohshiro ,  Satoshi Fukunaga

IPC分类号： G01N33/53 ,  B01J19/00

CPC分类号： G01N33/557 ,  G01N33/5306 ,  G01N33/558

摘要： Provided is a prozone phenomenon detecting method, by which generation of a prozone phenomenon can be detected even when a conventional specimen analysis tool is used, and examinations using an immunochromatography method and the like can be performed efficiently. In the method, a specimen analysis tool containing substances that specifically bind to a target component contained in a sample is used. The specimen analysis tool is obtained by arranging a sample supplying portion, a reagent portion, and a detection portion on the porous base material from upstream to downstream in a sample moving direction in this order. The reagent portion contains a labeled substance that specifically binds to the target component. The detection portion contains an immobilized substance that specifically binds to the target component. The target component is detected by detecting a complex of the target component, the labeled substance, and the immobilized substance through detection of a label of the labeled substance in the detection portion. The method includes at least one of the following processes A and B: the process A: a process in which detection results obtained in the detection portion are plotted along the sample moving direction, and generation of a prozone phenomenon is detected on the basis of a position of a peak in plots thus obtained; and the process B: a process in which the label is detected at two or more different time points in the detection portion, and generation of a prozone phenomenon is detected on the basis of a magnitude relationship between two or more detection results thus obtained.

摘要翻译： 提供了一种前区现象检测方法，即使使用常规的样本分析工具也能够检测到前区现象的产生，并且可以有效地执行使用免疫色谱法等的检查。 在该方法中，使用含有与试样中含有的靶成分特异结合的物质的试样分析工具。 样品分析工具通过在样品移动方向的上游到下游依次布置多孔基材上的样品供应部分，试剂部分和检测部分而获得。 试剂部分含有与目标成分特异性结合的标记物质。 检测部分含有特异性结合目标成分的固定化物质。 通过检测检测部中的标记物质的标签，检测目标成分，标记物质和固定化物质的复合物来检测目标成分。 该方法包括以下过程A和B中的至少一个：处理A：沿着样品移动方向绘制在检测部分中获得的检测结果的过程，并且基于 如此获得的图中峰的位置; 过程B：在检测部分中的两个或更多个不同时间点检测标签的过程，并且基于由此获得的两个或更多个检测结果之间的大小关系来检测前区现象的产生。

公开(公告)号：US08389230B2

公开(公告)日：2013-03-05

申请号：US12995075

申请日：2009-05-28

申请人： Kyouichi Ohshiro

发明人： Kyouichi Ohshiro

IPC分类号： G01N33/53

CPC分类号： G01N33/56983 ,  G01N21/78 ,  G01N21/8483 ,  G01N33/54373 ,  G01N33/56944 ,  G01N2021/7773

摘要： The present invention provides an immunoassay analyzer capable of discriminating between normal coloring due to a specific immunoreaction and abnormal coloring due to a cause other than the specific immunoreaction in a measurement region of a sample analysis tool. An immunoassay analyzer 1 of the present invention includes an optical detection unit 4 and a determination unit 5. The optical detection unit 4 includes an optical signal measurement unit for measuring an optical signal at each of two or more different wavelengths including a main wavelength for detecting color change due to the specific immunoreaction and a sub-wavelength(s) other than the main wavelength. The determination unit 5 includes a discrimination unit for comparing the respective optical signals at the two or more different wavelengths and discriminating between the color change due to the specific immunoreaction and color change due to a cause other than the specific immunoreaction based on a comparison criterion determined previously.

摘要翻译： 本发明提供一种能够鉴别由于特异性免疫反应引起的正常着色和由于在样品分析工具的测量区域中的特异性免疫反应以外的原因引起的异常着色的免疫测定分析仪。 本发明的免疫测定分析装置1包括光检测单元4和判定单元5.光检测单元4包括光信号测量单元，用于测量两个或多个不同波长中的每一个的光信号，包括用于检测的主波长 由于特定的免疫反应引起的颜色变化和除了主波长之外的亚波长。 确定单元5包括用于比较两个或更多个不同波长处的各个光信号的鉴别单元，并且基于所确定的比较标准来区分由于特异性免疫反应引起的颜色变化和由于特异性免疫反应以外的原因引起的颜色变化 先前。

公开(公告)号：US08426125B2

公开(公告)日：2013-04-23

申请号：US12949446

申请日：2010-11-18

申请人： Kyouichi Ohshiro ,  Kazuhiro Ohmiya ,  Naoko Izui

发明人： Kyouichi Ohshiro ,  Kazuhiro Ohmiya ,  Naoko Izui

IPC分类号： C12Q1/70 ,  C12Q1/00

CPC分类号： G01N33/56983 ,  G01N33/5306 ,  G01N2333/11 ,  G01N2333/95

摘要： The present invention provides a method of pretreating a specimen, which allows measurement according to an immunoassay to be carried out on a specimen from nasal secretion while preventing non-specific reactions. According to this method, the specimen from nasal secretion is treated with a protease beforehand and then an immunoassay is performed. As the protease, it is preferable to use semi-alkaline protease (EC 3.4.21.63). Furthermore, it is preferable that a substance to be pretreated by the pretreatment method according to the present invention is an influenza virus contained in the specimen from nasal secretion. The immunoassay preferably is an immunoagglutination assay. Examples of the immunoagglutination assay include a turbidimetric immunoassay, a latex turbidimetric immunoassay, and a latex agglutination assay that is performed on a slide glass.

摘要翻译： 本发明提供了一种预处理样品的方法，其允许根据免疫测定进行测量，以在防止非特异性反应的同时进行来自鼻分泌物的样品。 根据该方法，预先用蛋白酶处理来自鼻分泌物的样品，然后进行免疫测定。 作为蛋白酶，优选使用半碱性蛋白酶（EC 3.4.21.63）。 此外，优选通过本发明的预处理方法进行预处理的物质是来自鼻分泌物的样品中含有的流感病毒。 免疫测定优选是免疫凝集测定。 免疫凝集测定的实例包括比浊免疫测定，胶乳比浊免疫测定和在载玻片上进行的胶乳凝集测定。

公开(公告)号：US09417235B2

公开(公告)日：2016-08-16

申请号：US12593074

申请日：2008-11-20

申请人： Takashi Nakagawa ,  Shinya Nakajima ,  Tokuo Kasai ,  Kyouichi Ohshiro

发明人： Takashi Nakagawa ,  Shinya Nakajima ,  Tokuo Kasai ,  Kyouichi Ohshiro

IPC分类号： G01N21/84 ,  G01N21/25 ,  C12M1/34 ,  G01N33/52 ,  G01N33/543 ,  G01N33/558 ,  G01N33/50

CPC分类号： G01N33/54386 ,  G01N21/25 ,  G01N21/8483 ,  G01N33/52 ,  G01N33/558 ,  Y10T436/11 ,  Y10T436/12

摘要： An object is to provide an optical measurement apparatus for performing an efficient test by optical measurement without incurring incorrect measurements.To this end, the measurement apparatus utilizes a test instrument mounted thereto and including a carrier with a reagent retaining portion for application of a sample. The measurement apparatus includes a reader for color development at the reagent retaining portion, and a controller for driving control of the reader and for required determination. The controller performs the determination by utilizing the data obtained by reading the color development of the reagent after a reaction completion period Tr1-Tr6 depending on the reagent and starting from the mounting of the test instrument. When detecting that the color development at the reagent retaining portion is completed before the lapse of the reaction completion period Tr1-Tr6 after the mounting of the test instrument, the controller stops the test for the test instrument.

摘要翻译： 目的是提供一种用于通过光学测量执行有效测试的光学测量装置，而不会引起不正确的测量。 为此，测量装置使用安装在其上的测试仪器，并且包括具有用于施加样品的试剂保留部分的载体。 测量装置包括用于在试剂保持部分进行显色的读取器，以及用于驱动读取器的控制和所需确定的控制器。 控制器通过利用通过根据试剂读取反应完成时间Tr1-Tr6后的试剂的显色而获得的数据，并从测试仪器的安装开始。 当检测到在安装测试仪器之后经过反应完成时段Tr1-Tr6之前，试剂保持部分的显色已经完成时，控制器停止测试仪器的测试。

公开(公告)号：US20110058995A1

公开(公告)日：2011-03-10

申请号：US12593074

申请日：2008-11-20

申请人： Takashi Nakagawa ,  Shinya Nakajima ,  Tokuo Kasai ,  Kyouichi Ohshiro

发明人： Takashi Nakagawa ,  Shinya Nakajima ,  Tokuo Kasai ,  Kyouichi Ohshiro

IPC分类号： G01N31/22

CPC分类号： G01N33/54386 ,  G01N21/25 ,  G01N21/8483 ,  G01N33/52 ,  G01N33/558 ,  Y10T436/11 ,  Y10T436/12

摘要： An object is to provide an optical measurement apparatus for performing an efficient test by optical measurement without incurring incorrect measurements.To this end, the measurement apparatus utilizes a test instrument mounted thereto and including a carrier with a reagent retaining portion for application of a sample. The measurement apparatus includes a reader for color development at the reagent retaining portion, and a controller for driving control of the reader and for required determination. The controller performs the determination by utilizing the data obtained by reading the color development of the reagent after a reaction completion period Tr1-Tr6 depending on the reagent and starting from the mounting of the test instrument. When detecting that the color development at the reagent retaining portion is completed before the lapse of the reaction completion period Tr1-Tr6 after the mounting of the test instrument, the controller stops the test for the test instrument.

摘要翻译： 目的是提供一种用于通过光学测量执行有效测试的光学测量装置，而不会引起不正确的测量。 为此，测量装置使用安装在其上的测试仪器，并且包括具有用于施加样品的试剂保留部分的载体。 测量装置包括用于在试剂保持部分进行显色的读取器，以及用于驱动读取器的控制和所需确定的控制器。 控制器通过利用通过根据试剂读取反应完成时间Tr1-Tr6后的试剂的显色而获得的数据，并从测试仪器的安装开始。 当检测到在安装测试仪器之后经过反应完成时段Tr1-Tr6之前，试剂保持部分的显色已经完成时，控制器停止测试仪器的测试。

公开(公告)号：US06673632B1

公开(公告)日：2004-01-06

申请号：US09133942

申请日：1998-08-14

申请人： Kyouichi Ohshiro

发明人： Kyouichi Ohshiro

IPC分类号： G01N33536

CPC分类号： G01N33/5306 ,  G01N33/573

摘要： The present invention provides a method by which UTI concentration can be measured easily with high precision and good reproducibility. The measurement is performed by adding free anti-UTI antibodies to a sample and measuring the degree of the resulting agglutination, for example, from the change in absorbance. As shown in FIG. 3, the UTI concentration and the degree of the agglutination (i.e. the change in absorbance) are correlated. The absorbance can be measured by using a general spectrophotometer, preferably at a wavelength of 300 to 400 nm. Polyethylene glycol is preferably added to the reaction solution as an agglutination accelerator. The polyethylene glycol preferably has an average molecular weight of 2,000 to 20,000, and the concentration of polyethylene glycol in the reaction solution is preferably in the range of 2 to 10 weight %.

摘要翻译： 本发明提供了可以容易地以高精度和良好的再现性测量UTI浓度的方法。 通过向样品中加入游离抗UTI抗体并测量所得凝集的程度，例如，从吸光度的变化来进行测量。 如图所示。 如图3所示，UTI浓度和凝集度（即吸光度的变化）相关。 可以通过使用普通分光光度计测量吸光度，优选波长在300至400nm。 聚乙二醇优选作为凝集促进剂加入到反应溶液中。 聚乙二醇的平均分子量优选为2,000〜20,000，反应溶液中聚乙二醇的浓度优选为2〜10重量％。

公开(公告)号：US20110076695A1

公开(公告)日：2011-03-31

申请号：US12995075

申请日：2009-05-28

申请人： Kyouichi Ohshiro

发明人： Kyouichi Ohshiro

IPC分类号： G01N33/53 ,  B01J19/00 ,  C12M1/34

CPC分类号： G01N33/56983 ,  G01N21/78 ,  G01N21/8483 ,  G01N33/54373 ,  G01N33/56944 ,  G01N2021/7773

摘要： The present invention provides an immunoassay analyzer capable of discriminating between normal coloring due to a specific immunoreaction and abnormal coloring due to a cause other than the specific immunoreaction in a measurement region of a sample analysis tool. An immunoassay analyzer 1 of the present invention includes an optical detection unit 4 and a determination unit 5. The optical detection unit 4 includes an optical signal measurement unit for measuring an optical signal at each of two or more different wavelengths including a main wavelength for detecting color change due to the specific immunoreaction and a sub-wavelength(s) other than the main wavelength. The determination unit 5 includes a discrimination unit for comparing the respective optical signals at the two or more different wavelengths and discriminating between the color change due to the specific immunoreaction and color change due to a cause other than the specific immunoreaction based on a comparison criterion determined previously.

摘要翻译： 本发明提供一种能够鉴别由于特异性免疫反应引起的正常着色和由于在样品分析工具的测量区域中的特异性免疫反应以外的原因导致的异常着色的免疫测定分析仪。 本发明的免疫测定分析装置1包括光检测单元4和判定单元5.光检测单元4包括光信号测量单元，用于测量两个或多个不同波长中的每一个的光信号，包括用于检测的主波长 由于特定的免疫反应引起的颜色变化和除了主波长之外的亚波长。 确定单元5包括用于比较两个或更多个不同波长处的各个光信号的鉴别单元，并且基于所确定的比较标准来区分由于特异性免疫反应引起的颜色变化和由于特定免疫反应以外的原因引起的颜色变化 先前。

公开(公告)号：US20080166701A1

公开(公告)日：2008-07-10

申请号：US11885131

申请日：2006-07-24

申请人： Kazuhiro Ohmiya ,  Kyouichi Ohshiro

发明人： Kazuhiro Ohmiya ,  Kyouichi Ohshiro

IPC分类号： C12Q1/70 ,  G01N33/566 ,  G01N33/569 ,  C07K16/00

CPC分类号： G01N33/5306 ,  C07K16/1018 ,  C07K16/1275 ,  G01N33/54313 ,  G01N33/54393

摘要： In an immunoassay method using an antibody sensitization particle, the occurrence of false positives is prevented. The immunoassay method of the present invention is an immunoassay method for detecting or quantifying an analyte in a specimen by mixing an antibody sensitization particle obtained by sensitizing a particle with an antibody that reacts with the analyte in the specimen with the specimen, and measuring agglutination of the antibody sensitization particle by an antigen-antibody reaction, and the method includes pre-treating the specimen by using a cation exchange material in advance of the mixing step. As the cation exchange material, for example, a cation exchange material having a sulfonic acid group or a carboxyl group as a functional group can be used, and a form thereof is not limited particularly, and may be, for example, a cation exchange filter, a cation exchange column or the like.

摘要翻译： 在使用抗体致敏颗粒的免疫测定方法中，防止了假阳性的发生。 本发明的免疫测定方法是通过将通过使颗粒敏化获得的抗体致敏颗粒与与样品中的分析物反应的抗体与样品混合来检测或定量样品中的分析物的免疫测定方法， 通过抗原抗体反应的抗体致敏颗粒，并且该方法包括在混合步骤之前使用阳离子交换材料对样品进行预处理。 作为阳离子交换材料，例如可以使用具有磺酸基或羧基作为官能团的阳离子交换材料，其形式没有特别限制，可以是例如阳离子交换过滤器 ，阳离子交换柱等。