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1.发明授权Method for measuring the concentration of protease inhibitors, kit for use in such a method and method for dissolving a substrate 失效用于测量蛋白酶抑制剂浓度的方法，用于这种用于溶解底物的方法和方法的试剂盒
公开(公告)号：US5856117A
公开(公告)日：1999-01-05
申请号：US879962
申请日：1997-06-20
CPC分类号： C12Q1/37 , C12Q2337/12 , G01N2333/81 , G01N2333/811 , G01N2333/976 , Y10S435/963 , Y10S435/975
摘要： This invention provides a method for measuring the concentration of urinary trypsin inhibitors which is excellent in precision and reproducibility, whose operation is simple, and in which a possibility of damaging a plastic cell is eliminated. The method for measuring the concentration of urinary trypsin inhibitors comprises mixing an urine sample, a protease solution containing trypsin, and a buffer solution, adding a substrate solution to the mixture to cause the enzyme reaction, and measuring the activity of the enzyme, wherein the buffer solution is prepared so that it contains at least 0.15 .mu.mol calcium per 1 .mu.g of the trypsin but no more than 100 .mu.mol calcium per 1 ml of the urine sample in the reaction mixture, and wherein the substrate solution is prepared by dissolving the substrate in an organic solvent and diluting the mixture solution with aqueous medium, wherein at least one of an amphoteric surfactant and a nonionic surfactant is added to at least one of the organic solvent and the aqueous medium.
摘要翻译： 本发明提供了一种测定尿胰蛋白酶抑制剂浓度的方法，其精度和重复性优异，操作简单，消除了塑料细胞损伤的可能性。 用于测定尿胰蛋白酶抑制剂浓度的方法包括混合尿液样品，含有胰蛋白酶的蛋白酶溶液和缓冲溶液，向混合物中加入底物溶液以引起酶反应，并测量酶的活性，其中 制备缓冲液，使其每1ml胰蛋白酶含有至少0.15μmol钙，而在反应混合物中每1ml尿液含有不超过100μmol钙，其中底物溶液通过溶解 将所述底物置于有机溶剂中并用水性介质稀释所述混合溶液，其中至少一种两性表面活性剂和非离子表面活性剂加入至少一种有机溶剂和水性介质中。
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公开(公告)号：US5754288A
公开(公告)日：1998-05-19
申请号：US694342
申请日：1996-08-08
IPC分类号： G01J3/44 , G01N21/65 , G01N33/497
摘要： As to respective expiration components to be measured, wavelengths having excellent correlations between component concentrations and Raman spectral intensity values are previously selected as measuring wavelengths which are specific to the components, an expiration specimen is irradiated with Raman excitation light, Raman spectra at the measuring wavelength which is specific to nitrogen and those at the measuring wavelengths previously selected for the components to be measured respectively are measured, Raman spectral intensity ratios of the components to the Raman spectral intensity of nitrogen are obtained, and the respective expiration components are quantitatively analyzed through a calibration curve which is previously prepared as to the Raman spectral intensity ratios of the respective components to nitrogen and concentrations. It is possible to provide a measuring method utilizing Raman spectroscopy, which can directly determine intra- expiratory components in a short time with no requirement for expendable items.
摘要翻译： 对于要测量的各个到期组分，预先选择组分浓度和拉曼光谱强度值之间具有优异相关性的波长作为测定组分特有的波长，用拉曼激发光照射呼出样品，在测量波长处照射拉曼光谱 测量分别对于氮和特定于测量波长的测量波长的测量波长，并获得组分与氮的拉曼光谱强度的拉曼光谱强度比，并通过一个 校准曲线，其预先制备了各组分对氮和浓度的拉曼光谱强度比。 可以提供使用拉曼光谱的测量方法，其可以在短时间内直接确定呼气成分，而不需要消耗品。
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3.发明授权Method of and apparatus for measuring absorbance, component concentration or specific gravity of liquid sample 失效用于测量液体样品的吸光度，组分浓度或比重的方法和仪器
公开(公告)号：US5696580A
公开(公告)日：1997-12-09
申请号：US434717
申请日：1995-05-04
申请人： Hiroko Kubo , Xu Kexin , Harumi Uenoyama
发明人： Hiroko Kubo , Xu Kexin , Harumi Uenoyama
CPC分类号： G01N21/4133 , G01N21/0303
摘要： A measuring beam is incident upon a triangular cell storing a liquid sample, and transmitted light thereof is received by a linear sensor so that both transmitted light intensity and a position of the beam are detected. A refractive index calculating part calculates refractive indices from the position detected by the linear sensor, while an absorbance calculating part calculates absorbance values dependent on component concentration values correcting influence by transmittance change at the triangular cell through the transmitted light intensity values and the refractive indices of the sample calculated at the refractive index calculating part. A component concentration calculating part performs a multivariate analytical operation on the basis of the absorbance values dependent on the component concentration values at a plurality of measuring wavelengths calculated at the absorbance calculating part.
摘要翻译： 测量光束入射在存储液体样品的三角形单元上，并且其透射光被线性传感器接收，从而检测透射光强度和光束位置。 折射率计算部分从由线性传感器检测的位置计算折射率，而吸光度计算部分通过透射光强度值和透射光强度值来计算依赖于三角形单元的透射率变化校正影响的成分浓度值的吸光度值， 在折射率计算部分计算的样本。 成分浓度计算部根据在吸光度计算部计算出的多个测定波长的成分浓度值的吸光度值，进行多变量分析。
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公开(公告)号：US5332479A
公开(公告)日：1994-07-26
申请号：US883367
申请日：1992-05-15
申请人： Harumi Uenoyama , Hisashi Okuda
发明人： Harumi Uenoyama , Hisashi Okuda
IPC分类号： G01N27/327 , C12Q1/00 , G01N27/416 , G01N27/26
CPC分类号： C12Q1/004 , Y10S435/817
摘要： A biosensor which electrochemically detects a material which relates to a reaction of a specific compound in a liquid sample with a biologically active substance or its related substance, in which the biologically active material or an optionally used mediator is placed at a part which is remote from a position of an electrode which acts as electrochemical detector means, and optionally the biological active material or the mediator is covered with a polymer layer.
摘要翻译： 电化学检测与液体样品中的特定化合物与生物活性物质或其相关物质的反应相关的材料的生物传感器，其中生物活性物质或任选使用的介体放置在远离 充当电化学检测器装置的电极的位置，以及任选的生物活性材料或介体的聚合物层被覆盖。
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公开(公告)号：US06887430B1
公开(公告)日：2005-05-03
申请号：US08985816
申请日：1999-03-12
CPC分类号： G01N21/7703 , G01N21/552
摘要： A total reflection cell has a total reflection prism on at least one surface thereof. A mixed solution containing a gold colloid labelled antibody which is adsorbed on a gold colloid is stored in the total reflection cell, and a sample solution containing an antigen causing antigen-antibody reaction with the antibody is added thereto for forming a gold colloid labelled immune complex. A measuring beam is introduced into the total reflection prism from an incident optical system at an angle θ of incidence causing total reflection and an outgoing beam from the total reflection prism is received by a measuring optical system, thereby measuring absorption by the gold colloid labelled immune complex and carrying out qualification and determination of the antigen.
摘要翻译： 全反射单元在其至少一个表面上具有全反射棱镜。 将含有被吸附在金胶体上的金胶体标记抗体的混合溶液储存在全反射池中，向其中加入含抗原抗体与抗体反应的抗原的样品溶液，形成金胶体标记的免疫复合物 。 测量光束从入射光学系统以入射角度θ被引入全反射棱镜，从而引起全反射，并且来自全反射棱镜的输出光束被测量光学系统接收，从而测量金胶体标记的免疫吸收 复杂并进行抗原的鉴定和鉴定。
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6.发明授权公开(公告)号：US06612986B2
公开(公告)日：2003-09-02
申请号：US09800691
申请日：2001-03-07
IPC分类号： A61B500
CPC分类号： G06F19/3418 , G06F19/00 , G16H10/40 , G16H40/20 , G16H40/63 , Y10S128/904
摘要： A dispersed-type testing/measuring system provides for example a simplified terminal unit to be installed at a patient's home. The dispersed-type testing/measuring system has a central controlling unit 20 and a plurality of terminal units 30 each accessible to the central controlling unit via a communication line 15. Each terminal unit 30 includes a crude data collecting portion 40, and a data transmitting means 45 for sending the data collected by the crude data collecting portion 40 to the central controlling unit 20. The central controlling unit 20 includes a measurement data calculating means 210 for generating measurement data by making calculation on the crude data sent from each terminal unit 30.
摘要翻译： 分散型测试/测量系统例如提供了要安装在患者家中的简化终端单元。分散式测试/测量系统具有中央控制单元20和多个终端单元30，每个终端单元可访问中央控制 每个终端单元30包括原始数据收集部分40和用于将粗原始数据收集部分40收集的数据发送到中央控制单元20的数据发送装置45.中央控制单元20包括 测量数据计算装置210，用于通过对从每个终端单元30发送的原始数据进行计算来产生测量数据。
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公开(公告)号：US5772606A
公开(公告)日：1998-06-30
申请号：US397953
申请日：1995-03-03
申请人： Emi Ashibe , Takeshi Sakura , Harumi Uenoyama , Xu Kexin , Hiroko Kubo
发明人： Emi Ashibe , Takeshi Sakura , Harumi Uenoyama , Xu Kexin , Hiroko Kubo
IPC分类号： G01N21/35 , G01N33/483 , G01N33/493 , A61B5/00
CPC分类号： G01N33/493 , G01N21/359
摘要： A urinal is provided on its forward end with a cell, which internally communicates with the urinal to outwardly project from the same. A measuring part comprises a light source part for applying a measuring beam of the visible or near infrared wavelength region to the cell which is set in a cell setting part and a light receiving part for receiving and detecting the measuring beam transmitted through the cell, and measures absorbances of uric components to be measured at wavelengths selected therefor respectively. A sensor part is adapted to detect that the cell is set in the cell setting part, so that the measuring part starts a measuring operation on the basis of a signal indicating that the sensor detects the cell. It is possible to carry out a urine test on a person who lies on bed.
摘要翻译： 在其前端设有一个小便器，一个细胞，其内部与小便器通信以向外突出。 测量部分包括用于将可见光或近红外波长区域的测量光束施加到设置在单元设置部分中的单元的光源部分和用于接收和检测透过单元的测量光束的光接收部分，以及 测量在分别选择的波长处测量的尿液成分的吸光度。 传感器部分适于检测单元设置在单元设置部分中，使得测量部件基于表示传感器检测到单元的信号开始测量操作。 可以对躺在床上的人进行尿检。
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公开(公告)号：US5750410A
公开(公告)日：1998-05-12
申请号：US592522
申请日：1996-01-26
IPC分类号： G01N21/55 , G01N33/58 , G01N33/553
CPC分类号： G01N21/77 , G01N21/552 , G01N33/585 , Y10S435/962
摘要： A total reflection cell has a total reflection prism on at least one surface thereof. A mixed solution containing a gold colloid labelled antibody which is adsorbed on a gold colloid is stored in the total reflection cell, and a sample solution containing an antigen causing antigen-antibody reaction with the antibody is added thereto for forming a gold colloid labelled immune complex. A measuring beam is introduced into the total reflection prism from an incident optical system at an angle .theta. of incidence causing total reflection and an outgoing beam from the total reflection prism is received by a measuring optical system, thereby measuring absorption by the gold colloid labelled immune complex and carrying out qualification and determination of the antigen.
摘要翻译： 全反射单元在其至少一个表面上具有全反射棱镜。 将含有被吸附在金胶体上的金胶体标记抗体的混合溶液储存在全反射池中，向其中加入含抗原抗体与抗体反应的抗原的样品溶液，形成金胶体标记的免疫复合物 。 测量光束从入射光学系统以入射角度θ被引入全反射棱镜，从而引起全反射，并且来自全反射棱镜的输出光束被测量光学系统接收，从而测量金胶体标记的免疫吸收 复杂并进行抗原的鉴定和鉴定。
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公开(公告)号：US5741660A
公开(公告)日：1998-04-21
申请号：US594205
申请日：1996-01-30
CPC分类号： G01N21/65 , C12Q1/26 , G01N2021/651
摘要： As to reaction solutions in which the amount of each potassium ferricyanate, glucose oxidase and glucose substrate are set at a constant value while glucose concentrations are varied, intensity changes of peaks at a shift wavenumber of 2081 cm.sup.-1 in light scattering spectra are measured every 10 seconds, the maximum values of the intensity changes of the peaks at the respective glucose concentrations obtained, for obtaining a calibration curve from correlations between the maximum values of the reaction velocities and the glucose concentrations. The calibration curve is employed for carrying out quantitative measurement of an unknown sample.
摘要翻译： 对于在葡萄糖浓度变化时将铁氰化钾，葡萄糖氧化酶和葡萄糖底物的量设定为恒定值的反应溶液，每个测量光散射光谱中在2081cm -1的位移波数处的峰的强度变化 10秒，获得所得各葡萄糖浓度下峰的强度变化的最大值，以从反应速度的最大值与葡萄糖浓度之间的相关性获得校准曲线。 采用校准曲线进行未知样品的定量测量。
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公开(公告)号：US6147749A
公开(公告)日：2000-11-14
申请号：US817085
申请日：1997-04-29
申请人： Hiroko Kubo , Yoshio Mitsumura , Harumi Uenoyama , Kexin Xu
发明人： Hiroko Kubo , Yoshio Mitsumura , Harumi Uenoyama , Kexin Xu
CPC分类号： G01N21/27 , A61B5/14532 , A61B5/1455 , A61B5/6825 , A61B5/6842 , A61B5/6843
摘要： To project measuring light with good reproducibility onto the measuring part of a measured object so that measurement conditions can always be constant. An optical measuring apparatus is equipped with a spectroscopic analyzer that detects the spectral intensity of the reflected light of measuring light projected onto a human hand. Spectroscopic analyzer moves in the direction" of the X-axis, Y axis, and Z axis, and around the Z axis by a moving mechanism. During the registration of the measuring part of the human hand, a CCD camera takes a picture of the human hand, and the operator selects a part having a feature from the pattern of the taken image. During the spectrometry, the CCD camera again takes a picture of the human hand. The optical measuring apparatus then detects the registered feature part on the currently taken image, moves spectroscopic analyzer by moving mechanism, and projects measuring light onto the registered feature part.
摘要翻译： PCT No.PCT / JP96 / 02223 Sec。 371日期1997年4月7日 102（e）日期1997年4月7日PCT 1996年8月7日PCT PCT。 出版物WO97 / 06423 日期1997年2月20日将测量对象的重复性重新测量投影到测量对象的测量部位，以便测量条件始终保持不变。 光学测量装置配备有光谱分析仪，其检测投射到人手上的测量光的反射光的光谱强度。 光谱分析仪通过移动机构沿X轴，Y轴和Z轴方向移动，Z轴周围移动，在人体测量部位的配准期间，CCD摄像机拍摄 人手，操作员从拍摄图像的图案中选择具有特征的部分，在光谱测定期间，CCD照相机再次拍摄人手的照片，然后光学测量装置检测当前拍摄的注册特征部分 图像，通过移动机构移动光谱分析仪，并将测量光投射到注册的特征部分上。
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