公开(公告)号：US20230042456A1

公开(公告)日：2023-02-09

申请号：US17786864

申请日：2020-12-16

Applicant: BASF SE

Inventor： Hartwig SCHROEDER ,  Anne-Catrin HAILER ,  Birgit HOFF ,  Oskar ZELDER ,  Peter OEDMAN ,  Georg Benjamin WANDREY ,  Christian DIETZSCH ,  Mukesh KUMAR ,  Dominik SATORY ,  Matthew David BLANKSCHIEN ,  Jens Klaus PLASSMEIER

IPC: C12P19/04 ,  C12N9/88

Abstract: Increasing space-time-yield, carbon-conversion-efficiency and carbon substrate flexibility in the production of fine chemicals The inventors of the current invention have found a surprising positive effect of increased cAMP levels and/or manipulating the PTS system on the space-time-yield, carbon-conversion-efficiency and carbon substrate flexibility of fine chemical production of a host organism. This was achieved by de-regulating adenylate cyclase cyaa by deleting the C-terminal regulatory region leading to increased cAMP levels or deleting the Crr protein activity (carbohydrate repression resistance) which regulates the carbohydrate utilization system. Both lead to increased 2-fucosyllactoe and 6-sialyllactose production (human milk oligosaccharides) and increase carbohydrate usage.