公开(公告)号：US20170145451A1

公开(公告)日：2017-05-25

申请号：US15323515

申请日：2015-07-02

Applicant: BASF SE

Inventor： Kai-Uwe Baldenius ,  Michael Breuer ,  Klaus Ditrich ,  Vaidotas Navickas ,  Francesco Mutti ,  Tanja Knaus ,  Nicholas Turner

IPC: C12P13/00 ,  C12N9/04 ,  C12N9/06

CPC classification number: C12P13/001 ,  C12N9/0006 ,  C12N9/0016 ,  C12N9/0018 ,  C12Y101/01001 ,  C12Y104/01009 ,  C12Y104/0102

Abstract: The present invention relates to a novel biocatalytic method for the production of primary and secondary amines, comprising coupled enzymatic oxidation and reduction processes simultaneously regenerating the required cofactor; making use of two enzymes—i.e. an alcohol dehydrogenase and an amine dehydrogenase—operating simultaneously in a one pot process (i.e. biocatalytic cascade). Furthermore, the overall process is redox neutral, since the hydride generated from the first oxidative step is internally recycled in the second reductive step. The invention also relates to recombinant expression systems and microorganisms procuring the required enzyme activities; and bioreactors for performing such methods.