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公开(公告)号：US20190024038A1
公开(公告)日：2019-01-24
申请号：US16060626
申请日：2016-12-08
Applicant: BASF SE
Inventor： Katrin Treier-Marxen , Petra Deckert , Andreas Schaedler , Vaidotas Navickas , Volker Wengert , Thomas Kaeding
Abstract: The present invention is directed to a method of purifying a protein of interest from the particulate matter of a fermentation broth comprising the step of purifying the protein of interest from the particulate matter of the fermentation broth, wherein the step of purifying the protein of interest from the particulate matter of the fermentation broth comprises one or more washing steps, which comprises contacting the particulate matter with a washing solution comprising one or more conditions that favor the solubilization of the protein of interest and/or the desorption of the protein of interest from the particulate matter.
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公开(公告)号：US20160237170A1
公开(公告)日：2016-08-18
申请号：US15024387
申请日：2014-10-02
Applicant: BASF SE
Inventor： Frank Rittig , Michael Koch , Vaidotas Navickas , Stefan Koch , Kindler Alois
CPC classification number: C08B1/00 , C08H8/00 , C12P7/10 , C12P7/16 , C12P7/46 , C12P19/02 , C12P19/14 , C12P2201/00 , Y02E50/10 , Y02E50/16
Abstract: Described are a method for processing cellulose-containing biomass and the use of methanesulfonic acid for processing cellulose-containing biomass, especially for the pretreatment of cellulose-containing biomass prior to saccharification.
Abstract translation: 描述了一种处理含纤维素生物质的方法，以及使用甲磺酸处理含纤维素的生物质，特别是用于在糖化之前预处理含纤维素的生物质。
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公开(公告)号：US20240191175A1
公开(公告)日：2024-06-13
申请号：US18443753
申请日：2024-02-16
Applicant: BASF SE
Inventor： Katrin Treier-Marxen , Petra Deckert , Andreas Schaedler , Vaidotas Navickas , Volker Wengert , Thomas Kaeding
CPC classification number: C12N1/02 , B01D61/147 , B01D61/16 , C07K1/145 , C07K1/30 , C12P21/02 , B01D2311/04 , B01D2311/18 , B01D2315/16
Abstract: The present invention is directed to a method of purifying a protein of interest from the particulate matter of a fermentation broth comprising the step of purifying the protein of interest from the particulate matter of the fermentation broth, wherein the step of purifying the protein of interest from the particulate matter of the fermentation broth comprises one or more washing steps, which comprises contacting the particulate matter with a washing solution comprising one or more conditions that favor the solubilization of the protein of interest and/or the desorption of the protein of interest from the particulate matter.
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公开(公告)号：US10533201B2
公开(公告)日：2020-01-14
申请号：US15509993
申请日：2015-06-30
Applicant: BASF SE
Inventor： Vaidotas Navickas , Kai-Uwe Baldenius , Michael Breuer , Patricia Wildberger , Martin Pfeiffer , Bernd Nidetzky
Abstract: The present invention relates to methods of producing sugar phosphates by enzymatic transphosphorylation, wherein the phosphoryl transfer from phosphate donor substrates to sugar substrates is catalyzed by an enzyme having alpha-glucose-1-phosphatase activity. Furthermore, the present invention relates to a biocatalyst having sucrose phosphorylase as well as alpha-glucose-1-phosphatase activity and its use. The production of an enzyme having alpha-glucose-1-phosphatase activity and its use in the transphosphorylation of sugar substrates is also presented herein.
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Patent Agency Ranking
公开(公告)号：US10428361B2
公开(公告)日：2019-10-01
申请号：US15559962
申请日：2016-02-19
Applicant: BASF SE
Inventor： Vaidotas Navickas , Michael Breuer , Michael Puhl , Melanie Weingarten , Kai-Uwe Baldenius , Wolfgang Siegel
Abstract: The present invention relates to biocatalytic methods, comprising purely enzymatic, mixed enzymatic-fermentative and purely fermentative methods, for the direct single-step conversion of L-fucitol to L-fucose, in order to easily obtain L-fucose at high amounts and levels of purity. Suitable recombinant microorganisms and fungi are further disclosed and also the use thereof in said method for the single-step conversion to L-fucose.
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公开(公告)号：US10337144B2
公开(公告)日：2019-07-02
申请号：US15552522
申请日：2016-02-18
Applicant: BASF SE
Inventor： Frank Rittig , Stefan Koch , Alois Kindler , Michael Koch , Ferdinand Leifeld , Vaidotas Navickas , Markus Gruen
Abstract: Described is a method for processing cellulose-containing biomass with sulfuric acid and certain additives, especially for the pretreatment of cellulose-containing biomass prior to saccharification.
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公开(公告)号：US10316106B2
公开(公告)日：2019-06-11
申请号：US15552521
申请日：2016-02-18
Applicant: BASF SE
Inventor： Frank Rittig , Stefan Koch , Alois Kindler , Michael Koch , Ferdinand Leifeld , Vaidotas Navickas , Markus Gruen
Abstract: Described is a method for processing cellulose-containing biomass with sulfuric acid and certain additives, especially for the pretreatment of cellulose-containing biomass prior to saccharification.
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公开(公告)号：US09822187B2
公开(公告)日：2017-11-21
申请号：US15024387
申请日：2014-10-02
Applicant: BASF SE
Inventor： Frank Rittig , Michael Koch , Vaidotas Navickas , Stefan Koch , Alois Kindler
CPC classification number: C08B1/00 , C08H8/00 , C12P7/10 , C12P7/16 , C12P7/46 , C12P19/02 , C12P19/14 , C12P2201/00 , Y02E50/10 , Y02E50/16
Abstract: Described are a method for processing cellulose-containing biomass and the use of methanesulfonic acid for processing cellulose-containing biomass, especially for the pretreatment of cellulose-containing biomass prior to saccharification.
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公开(公告)号：US20170145451A1
公开(公告)日：2017-05-25
申请号：US15323515
申请日：2015-07-02
Applicant: BASF SE
Inventor： Kai-Uwe Baldenius , Michael Breuer , Klaus Ditrich , Vaidotas Navickas , Francesco Mutti , Tanja Knaus , Nicholas Turner
CPC classification number: C12P13/001 , C12N9/0006 , C12N9/0016 , C12N9/0018 , C12Y101/01001 , C12Y104/01009 , C12Y104/0102
Abstract: The present invention relates to a novel biocatalytic method for the production of primary and secondary amines, comprising coupled enzymatic oxidation and reduction processes simultaneously regenerating the required cofactor; making use of two enzymes—i.e. an alcohol dehydrogenase and an amine dehydrogenase—operating simultaneously in a one pot process (i.e. biocatalytic cascade). Furthermore, the overall process is redox neutral, since the hydride generated from the first oxidative step is internally recycled in the second reductive step. The invention also relates to recombinant expression systems and microorganisms procuring the required enzyme activities; and bioreactors for performing such methods.
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