公开(公告)号：US20160107992A1

公开(公告)日：2016-04-21

申请号：US14894770

申请日：2014-06-18

申请人： CHEMELECTIVA S.R.L

发明人： Graziano CASTALDI ,  Giuseppe PALLANZA ,  Mauro GABOARDI ,  Marta CASTALDI

IPC分类号： C07D209/52 ,  C07D207/22

CPC分类号： C07D209/52 ,  C07D207/22 ,  C12P13/02 ,  C12P17/10 ,  C12Y104/0102

摘要： Intermediates useful for the synthesis of saxagliptin and their preparationare described.

摘要翻译： 描述了用于合成沙格列汀及其制备的中间体。

公开(公告)号：US20170145451A1

公开(公告)日：2017-05-25

申请号：US15323515

申请日：2015-07-02

申请人： BASF SE

发明人： Kai-Uwe Baldenius ,  Michael Breuer ,  Klaus Ditrich ,  Vaidotas Navickas ,  Francesco Mutti ,  Tanja Knaus ,  Nicholas Turner

IPC分类号： C12P13/00 ,  C12N9/04 ,  C12N9/06

CPC分类号： C12P13/001 ,  C12N9/0006 ,  C12N9/0016 ,  C12N9/0018 ,  C12Y101/01001 ,  C12Y104/01009 ,  C12Y104/0102

摘要： The present invention relates to a novel biocatalytic method for the production of primary and secondary amines, comprising coupled enzymatic oxidation and reduction processes simultaneously regenerating the required cofactor; making use of two enzymes—i.e. an alcohol dehydrogenase and an amine dehydrogenase—operating simultaneously in a one pot process (i.e. biocatalytic cascade). Furthermore, the overall process is redox neutral, since the hydride generated from the first oxidative step is internally recycled in the second reductive step. The invention also relates to recombinant expression systems and microorganisms procuring the required enzyme activities; and bioreactors for performing such methods.

公开(公告)号：US20160153015A1

公开(公告)日：2016-06-02

申请号：US14782586

申请日：2013-06-27

申请人： ASYMCHEM LABORATORIES (TIANJIN) CO., LTD ,  ASYMCHEM LIFE SCIENCE (TIANJIN) CO., LTD ,  TIANJIN ASYMCHEM PHARMACEUTICAL CO., LTD ,  ASYMCHEM LABORATORIES (FUXIN) CO., LTD ,  JILIN ASYMCHEM LABORATORIES CO., LTD

发明人： Hao HONG ,  Changsheng ZHENG ,  Lina GUO

IPC分类号： C12P17/12 ,  C12P17/00 ,  A61K31/4409 ,  A61K38/44 ,  A61K31/4406 ,  A61K31/415 ,  A61K31/422 ,  C12P17/10 ,  A61K31/4402

CPC分类号： C12P17/12 ,  A61K31/415 ,  A61K31/422 ,  A61K31/4402 ,  A61K31/4406 ,  A61K31/4409 ,  A61K38/44 ,  A61K48/00 ,  C07D213/55 ,  C07D231/12 ,  C07D333/24 ,  C12P13/04 ,  C12P13/22 ,  C12P17/00 ,  C12P17/10 ,  C12P41/006 ,  C12Y102/01002 ,  C12Y104/0102

摘要： A synthesis method for an L-heterocyclic amino acid and a pharmaceutical composition having the said amino acid are provided in the present disclosure. The synthesis method comprises: step A: preparing a heterocyclic keto acid, wherein the heterocycle in the heterocyclic keto acid is selected from any one of a five-membered heterocycle, a six-membered heterocycle, a seven-membered heterocycle, an alkyl-substituted five-membered heterocycle, an alkyl-substituted six-membered heterocycle, and an alkyl-substituted seven-membered heterocycle, and the keto acid group in the heterocyclic keto acid has a structural formula of and is located on any one of the carbon positions of the heterocycle, and step B: mixing the heterocyclic keto acid with ammonium formate, a phenylalanine dehydrogenase, a formate dehydrogenase and a coenzyme NAD+, and carrying out a reductive amination reaction to generate L-heterocyclic amino acid, wherein the amino acid sequence of the phenylalanine dehydrogenase is SEQ ID No. 1.

摘要翻译： 在本公开内容中提供了具有所述氨基酸的L-杂环氨基酸的合成方法和药物组合物。 合成方法包括：步骤A：制备杂环酮酸，其中杂环酮酸中的杂环选自五元杂环，六元杂环，七元杂环，烷基取代的 五元杂环，烷基取代的六元杂环和烷基取代的七元杂环，并且杂环酮酸中的酮酸基具有结构式，位于任何一个碳位上 杂环和步骤B：将杂环酮酸与甲酸铵，苯丙氨酸脱氢酶，甲酸脱氢酶和辅酶NAD +混合，并进行还原胺化反应以产生L-杂环氨基酸，其中氨基酸序列 苯丙氨酸脱氢酶是SEQ ID No.1。