公开(公告)号：US20240361214A1

公开(公告)日：2024-10-31

申请号：US18754887

申请日：2024-06-26

Applicant: BD KIESTRA B.V.

Inventor： Timothy Roy Hansen ,  Martijn Kleefstra ,  Michael Bois ,  Timothy M. Wiles ,  Charles Chak Cheung Yu

IPC: G01N1/38 ,  C12Q1/18 ,  C12Q1/24 ,  G01N1/40

CPC classification number: G01N1/38 ,  C12Q1/18 ,  C12Q1/24 ,  G01N1/40 ,  G01N2001/386

Abstract: An automated method for preparing a sample suspension. The sample suspension can be used for both MALDI and antimicrobial susceptibility (AST). A suspension is prepared, and a portion of that suspension is removed for a first analysis (e.g. MALDI), leaving a remaining volume. The turbidity of the remaining volume is measured. If the turbidity is below a first threshold, the suspension is not used for a second analysis (e.g. AST) and is subjected to a concentration protocol to raise the turbidity of the suspension. If the turbidity is within a predetermined range, a volume of the suspension is calculated that will deliver a predetermined amount of sample to a vessel for the second analysis. If the turbidity of the suspension is above the predetermined range, and the suspension has not been diluted a predetermined number of times, the suspension is diluted according to a dilution protocol.

公开(公告)号：US12038359B2

公开(公告)日：2024-07-16

申请号：US17255299

申请日：2019-06-24

Applicant: BD KIESTRA B.V.

Inventor： Timothy Roy Hansen ,  Martijn Kleefstra ,  Michael Bois ,  Timothy M. Wiles ,  Charles Chak Cheung Yu

IPC: G01N1/38 ,  C12Q1/18 ,  C12Q1/24 ,  G01N1/40

CPC classification number: G01N1/38 ,  C12Q1/18 ,  C12Q1/24 ,  G01N1/40 ,  G01N2001/386

Abstract: An automated method for preparing a sample suspension. The sample suspension can be used for both MALDI and antimicrobial susceptibility (AST). A suspension is prepared, and a portion of that suspension is removed for a first analysis (e.g. MALDI), leaving a remaining volume. The turbidity of the remaining volume is measured. If the turbidity is below a first threshold, the suspension is not used for a second analysis (e.g. AST) and is subjected to a concentration protocol to raise the turbidity of the suspension. If the turbidity is within a predetermined range, a volume of the suspension is calculated that will deliver a predetermined amount of sample to a vessel for the second analysis. If the turbidity of the suspension is above the predetermined range, and the suspension has not been diluted a predetermined number of times, the suspension is diluted according to a dilution protocol.

公开(公告)号：US20210199546A1

公开(公告)日：2021-07-01

申请号：US17255299

申请日：2019-06-24

Applicant: BD KIESTRA B.V.

Inventor： Timothy Roy Hansen ,  Martijn Kleefstra ,  Michael Bois ,  Timothy M. Wiles ,  Charles Chak Cheung Yu

IPC: G01N1/38 ,  C12Q1/18 ,  C12Q1/24 ,  G01N1/40

Abstract: An automated method for preparing a sample suspension. The sample suspension can be used for both MALDI and antimicrobial susceptibility (AST). A suspension is prepared, and a portion of that suspension is removed for a first analysis (e.g. MALDI), leaving a remaining volume. The turbidity of the remaining volume is measured. If the turbidity is below a first threshold, the suspension is not used for a second analysis (e.g. AST) and is subjected to a concentration protocol to raise the turbidity of the suspension. If the turbidity is within a predetermined range, a volume of the suspension is calculated that will deliver a predetermined amount of sample to a vessel for the second analysis. If the turbidity of the suspension is above the predetermined range, and the suspension has not been diluted a predetermined number of times, the suspension is diluted according to a dilution protocol.