公开(公告)号：US11649489B2

公开(公告)日：2023-05-16

申请号：US16757719

申请日：2018-10-12

Applicant: BGI SHENZHEN

Inventor： Erkai Liu ,  Wenwei Zhang ,  Ao Chen ,  Chongjun Xu

IPC: C12Q1/68 ,  C12Q1/6869 ,  C12Q1/6876

CPC classification number: C12Q1/6869 ,  C12Q1/6876

Abstract: Provided are a nucleic acid sequencing method and a nucleic acid sequencing kit. The kit comprises a nucleic acid probe, a ligase, dNTP having a blocking group attached to a 3′ end, a polymerase, a reagent 1 for excising the blocking group attached to the 3′ end of the dNTP, and a reagent 2 for excising the remaining nucleotides on the nucleic acid probe that are not bound to a to-be-tested base group.