公开(公告)号：US11649489B2

公开(公告)日：2023-05-16

申请号：US16757719

申请日：2018-10-12

Applicant: BGI SHENZHEN

Inventor： Erkai Liu ,  Wenwei Zhang ,  Ao Chen ,  Chongjun Xu

IPC: C12Q1/68 ,  C12Q1/6869 ,  C12Q1/6876

CPC classification number: C12Q1/6869 ,  C12Q1/6876

Abstract: Provided are a nucleic acid sequencing method and a nucleic acid sequencing kit. The kit comprises a nucleic acid probe, a ligase, dNTP having a blocking group attached to a 3′ end, a polymerase, a reagent 1 for excising the blocking group attached to the 3′ end of the dNTP, and a reagent 2 for excising the remaining nucleotides on the nucleic acid probe that are not bound to a to-be-tested base group.

公开(公告)号：US20210340509A1

公开(公告)日：2021-11-04

申请号：US17358856

申请日：2021-06-25

Applicant: BGI SHENZHEN

Inventor： Huanhuan Liu ,  Na Guo ,  Huizhen Li ,  Zhougang Zhang ,  Hongyan Han ,  Miaomiao Guo ,  Yue Zheng ,  Yuliang Dong ,  Wenwei Zhang ,  Chongjun Xu

IPC: C12N9/12 ,  C12N15/10 ,  C12Q1/6869 ,  C12N15/70 ,  C12Q1/686 ,  C12Q1/6806

Abstract: The present disclosure relates to a reverse transcriptase and an application thereof. The reverse transcriptase has mutation sites such as R450H compared with the wild-type M-MLV reverse transcriptase. The reverse transcriptase has increased polymerase activity, improved thermal stability, and reduced RNase H activity.