公开(公告)号：US10563196B2

公开(公告)日：2020-02-18

申请号：US15519530

申请日：2014-10-17

Applicant: BGI SHENZHEN

Inventor： Chunyu Geng ,  Hongyan Han ,  Guangying Guo ,  Wenwei Zhang ,  Hui Jiang ,  Yuan Jiang

IPC: C12N15/10 ,  C12Q1/6806 ,  C12Q1/68 ,  C12Q1/6853 ,  C12Q1/686 ,  C40B40/08 ,  C40B50/06

Abstract: The present invention provides a primer for nucleic acid random fragmentation and a nucleic acid random fragmentation method. The primer consists of a plurality of upstream random primers and downstream random primers. The sequence composition of the upstream random primers is 5′-X-Y-3′, and the sequence composition of the downstream random primers is 5′-P-Y′-X′-close-3′, wherein Y and Y′ are random sequences, X is all or part of sequences of a sequencing platform 5′ end adaptor, X′ is all or part of sequences of a sequencing platform 3′ end adaptor, P is phosphorylation modification, and close is close modification. The primer of the present invention adopts double random anchoring of both the upstream random primers and the downstream random primers, and a DNA sample can be randomly broken.