公开(公告)号：US10563196B2

公开(公告)日：2020-02-18

申请号：US15519530

申请日：2014-10-17

Applicant: BGI SHENZHEN

Inventor： Chunyu Geng ,  Hongyan Han ,  Guangying Guo ,  Wenwei Zhang ,  Hui Jiang ,  Yuan Jiang

IPC: C12N15/10 ,  C12Q1/6806 ,  C12Q1/68 ,  C12Q1/6853 ,  C12Q1/686 ,  C40B40/08 ,  C40B50/06

Abstract: The present invention provides a primer for nucleic acid random fragmentation and a nucleic acid random fragmentation method. The primer consists of a plurality of upstream random primers and downstream random primers. The sequence composition of the upstream random primers is 5′-X-Y-3′, and the sequence composition of the downstream random primers is 5′-P-Y′-X′-close-3′, wherein Y and Y′ are random sequences, X is all or part of sequences of a sequencing platform 5′ end adaptor, X′ is all or part of sequences of a sequencing platform 3′ end adaptor, P is phosphorylation modification, and close is close modification. The primer of the present invention adopts double random anchoring of both the upstream random primers and the downstream random primers, and a DNA sample can be randomly broken.

公开(公告)号：US10344317B2

公开(公告)日：2019-07-09

申请号：US15518760

申请日：2014-10-13

Applicant: BGI SHENZHEN CO., LIMITED

Inventor： Hongyan Han ,  Chunyu Geng ,  Guanying Guo ,  Wenwei Zhang ,  Hui Jiang ,  Yuan Jiang

IPC: C12P19/34 ,  C12Q1/6806 ,  C12Q1/6855 ,  C12Q1/686

Abstract: Disclosed are a nucleic acid fragmentation method and a sequence combination. The method comprises the following steps: subjecting a denatured nucleic acid to annealing and an extension reaction by using a single-stranded 5′-end extension primer, wherein the single-stranded 5′-end extension primer comprises a sequencing platform adaptor sequence of a 5′ end and a connected random sequence, and the random sequence is subjected to annealing on a random site of the denatured nucleic acid; and directionally connecting a double-stranded 3′-end adaptor sequence to the 3′ end of the nucleic acid generated in the extension reaction, and carrying out denaturalization and purification to obtain a fragmented single-stranded nucleic acid with adaptor sequences on two ends.

公开(公告)号：US20210340509A1

公开(公告)日：2021-11-04

申请号：US17358856

申请日：2021-06-25

Applicant: BGI SHENZHEN

Inventor： Huanhuan Liu ,  Na Guo ,  Huizhen Li ,  Zhougang Zhang ,  Hongyan Han ,  Miaomiao Guo ,  Yue Zheng ,  Yuliang Dong ,  Wenwei Zhang ,  Chongjun Xu

IPC: C12N9/12 ,  C12N15/10 ,  C12Q1/6869 ,  C12N15/70 ,  C12Q1/686 ,  C12Q1/6806

Abstract: The present disclosure relates to a reverse transcriptase and an application thereof. The reverse transcriptase has mutation sites such as R450H compared with the wild-type M-MLV reverse transcriptase. The reverse transcriptase has increased polymerase activity, improved thermal stability, and reduced RNase H activity.