公开(公告)号：US20230056313A1

公开(公告)日：2023-02-23

申请号：US18048671

申请日：2022-10-21

Applicant: BGI SHENZHEN

Inventor： Zhaohui Wang ,  Handong Li ,  Yuan Li ,  Wenwei Zhang ,  Ao Chen

IPC: C12Q1/6874 ,  H01L27/146 ,  B01L3/00

Abstract: Provided is a sequencing chip. The sequencing chip includes: a chip main body, nucleic acids, and a phosphonic acid polymer film. The chip main body includes a plurality of chip particles arranged in a same layer, the chip particles are obtained by cutting a chip matrix along cutting lines of a wafer layer, and the chip matrix includes: the wafer layer having the cutting lines uniformly distributed thereon; a first silicon oxide layer made of silicon oxide and formed on an upper surface of the wafer layer; and a transition metal oxide layer made of a transition metal oxide and formed on an upper surface of the first silicon oxide layer. The nucleic acids are fixed on the transition metal oxide layer; and the phosphonic acid polymer film is made of a polyphosphonic acid polymer and formed on an upper surface of the transition metal oxide layer.

公开(公告)号：US20210384031A1

公开(公告)日：2021-12-09

申请号：US17377114

申请日：2021-07-15

Applicant: BGI SHENZHEN

Inventor： Shifeng Li ,  Tengyue Li ,  Yuan Li ,  Zhaohui Wang ,  Xueqin Jiang ,  Jiacheng Chen ,  Aoli Wang ,  Fuxing Huang ,  Xiaogang Song ,  Lingling Peng ,  Handong Li ,  Wenwei Zhang

IPC: H01L21/02 ,  H01L21/78

Abstract: Provided are a chip matrix, a sequencing chip, and a manufacturing method thereof. The chip matrix includes: a wafer layer (111), the wafer layer (111) having cutting lines that are evenly distributed thereon; a first silicon oxide layer (112), the first silicon oxide layer (112) being made of silicon oxide and formed on an upper surface of the wafer layer (111); a transition metal oxide layer (113), the transition metal oxide layer (113) being made of transition metal oxide and formed on an upper surface of the first silicon oxide layer (112). The chip matrix has characteristics such as resistances against high temperature, high humidity and other harsh environments. Meanwhile, by changing pH, surfactant and other components of a solution containing sequences to be sequenced, a surface functional region of the chip matrix can specifically adsorb a sequence to be sequenced.