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    METHODS AND COMPOSITIONS FOR BIOLUMINESCENCE-BASED SEQUENCING
    5.
    发明公开
    METHODS AND COMPOSITIONS FOR BIOLUMINESCENCE-BASED SEQUENCING 审中-公开

    公开(公告)号：US20230366023A1

    公开(公告)日：2023-11-16

    申请号：US18246959

    申请日：2021-10-09

    Applicant: BGI Shenzhen Co., Ltd.

    Inventor： Yan Chen ,  Handong Li ,  Yongwei Zhang

    IPC: C12Q1/6874

    CPC classification number: C12Q1/6874

    Abstract: Disclosed herein are methods and compositions for corresponding positions on an array with differently labeled affinity reagents immobilized at the positions. A first and a second affinity reagents are immobilized on at least some of the first and second positions, respectively. The first affinity reagent and the second affinity reagent are associated with a first luciferase polypeptide and a second luciferase polypeptide, respectively. The first luciferase polypeptide does not cross react with the second substrate and the second luciferase polypeptide does not cross react with the first substrate. The method further comprises contacting the array with the first substrate, which reacts with the first luciferase polypeptide and detecting the first luminescent signal and detecting the second luminescent signal at positions, thereby corresponding positions on an array with the first affinity reagent or the second affinity reagent immobilized at the positions.

    SEQUENCING CHIP AND PREPARATION METHOD THEREFOR
    6.
    发明申请
    SEQUENCING CHIP AND PREPARATION METHOD THEREFOR 有权

    公开(公告)号：US20230056313A1

    公开(公告)日：2023-02-23

    申请号：US18048671

    申请日：2022-10-21

    Applicant: BGI SHENZHEN

    Inventor： Zhaohui Wang ,  Handong Li ,  Yuan Li ,  Wenwei Zhang ,  Ao Chen

    IPC: C12Q1/6874 ,  H01L27/146 ,  B01L3/00

    Abstract: Provided is a sequencing chip. The sequencing chip includes: a chip main body, nucleic acids, and a phosphonic acid polymer film. The chip main body includes a plurality of chip particles arranged in a same layer, the chip particles are obtained by cutting a chip matrix along cutting lines of a wafer layer, and the chip matrix includes: the wafer layer having the cutting lines uniformly distributed thereon; a first silicon oxide layer made of silicon oxide and formed on an upper surface of the wafer layer; and a transition metal oxide layer made of a transition metal oxide and formed on an upper surface of the first silicon oxide layer. The nucleic acids are fixed on the transition metal oxide layer; and the phosphonic acid polymer film is made of a polyphosphonic acid polymer and formed on an upper surface of the transition metal oxide layer.

    SEQUENCING CHIP AND MANUFACTURING METHOD THEREFOR
    7.
    发明申请
    SEQUENCING CHIP AND MANUFACTURING METHOD THEREFOR 有权

    公开(公告)号：US20210384031A1

    公开(公告)日：2021-12-09

    申请号：US17377114

    申请日：2021-07-15

    Applicant: BGI SHENZHEN

    Inventor： Shifeng Li ,  Tengyue Li ,  Yuan Li ,  Zhaohui Wang ,  Xueqin Jiang ,  Jiacheng Chen ,  Aoli Wang ,  Fuxing Huang ,  Xiaogang Song ,  Lingling Peng ,  Handong Li ,  Wenwei Zhang

    IPC: H01L21/02 ,  H01L21/78

    Abstract: Provided are a chip matrix, a sequencing chip, and a manufacturing method thereof. The chip matrix includes: a wafer layer (111), the wafer layer (111) having cutting lines that are evenly distributed thereon; a first silicon oxide layer (112), the first silicon oxide layer (112) being made of silicon oxide and formed on an upper surface of the wafer layer (111); a transition metal oxide layer (113), the transition metal oxide layer (113) being made of transition metal oxide and formed on an upper surface of the first silicon oxide layer (112). The chip matrix has characteristics such as resistances against high temperature, high humidity and other harsh environments. Meanwhile, by changing pH, surfactant and other components of a solution containing sequences to be sequenced, a surface functional region of the chip matrix can specifically adsorb a sequence to be sequenced.

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