公开(公告)号：US20230160884A1

公开(公告)日：2023-05-25

申请号：US18099224

申请日：2023-01-19

Applicant: BIOLEGEND, INC.

Inventor： DHANESH GOHEL ,  HONG ZHANG ,  JOHN RANSOM

IPC: G01N33/543 ,  G01N33/552

CPC classification number: G01N33/54326 ,  G01N33/552

Abstract: Processes and compositions are described for preparing new, colloidally stable, coated nanomagnetic particles useful for both in-vitro and in-vivo biomedical applications, including cell targeting and capturing cells, microorganisms, and cellular organelles or entities such as exosomes. These nanomagnetic particles can also be used as imaging contrast agents due to their small size and high magnetic moment. The nanomagnetic particles include a series of sequentially added, stabilizing surface coatings rendered onto nano-sized magnetic crystal clusters (e.g., magnetite particles) to impart colloidal stability in complex biological samples with minimal leaching of the coating materials, high binding capacity, and low non-specific binding. Another benefit of this invention is the ability to utilize both external and internal magnetic field-generating separation devices to effect separation of the magnetic nanoparticles.

公开(公告)号：US20230272344A1

公开(公告)日：2023-08-31

申请号：US18099233

申请日：2023-01-19

Applicant: BIOLEGEND, INC.

Inventor： DHANESH GOHEL ,  HONG ZHANG ,  JOHN RANSOM

IPC: C12N5/0789 ,  G01N33/543 ,  C12N15/10 ,  G01N33/552 ,  B01J20/06 ,  B01J20/10 ,  B01J20/24 ,  B01J20/28 ,  C07K16/28 ,  C12N5/0783 ,  A61K35/28 ,  B01J20/32 ,  B03C1/015 ,  B03C1/28

CPC classification number: C12N5/0647 ,  A61K35/28 ,  B01J20/06 ,  B01J20/10 ,  B01J20/103 ,  B01J20/24 ,  B01J20/28007 ,  B01J20/28009 ,  B01J20/28021 ,  B01J20/3204 ,  B01J20/3217 ,  B01J20/3236 ,  B01J20/328 ,  B01J20/3289 ,  B01J20/3293 ,  B03C1/015 ,  B03C1/28 ,  C07K16/2812 ,  C12N5/0637 ,  C12N15/1013 ,  G01N33/54326 ,  G01N33/54333 ,  G01N33/54346 ,  G01N33/552 ,  B03C2201/18 ,  B03C2201/26

Abstract: The methods of the invention employ targeted magnetic particles, preferably targeted nanomagnetic particles, and targeted buoyant particles such as buoyant microparticles and microbubbles. Among the benefits of the invention is the ability to combine targeted magnetic particles with differentially targeted buoyant particles to achieve separation of two or more specifically cell targeted populations during the same work flow.