公开(公告)号：US20110266225A1

公开(公告)日：2011-11-03

申请号：US13158780

申请日：2011-06-13

申请人： BO-LENNART JOHANSSON ,  JEAN-LUC MALOISEL ,  HANS J. JOHANSSON ,  ASA EK ,  MARIA HOLM ,  JAFAR SAIDZADEH

发明人： BO-LENNART JOHANSSON ,  JEAN-LUC MALOISEL ,  HANS J. JOHANSSON ,  ASA EK ,  MARIA HOLM ,  JAFAR SAIDZADEH

IPC分类号： B01D15/08 ,  B23P17/04

CPC分类号： B01D15/327 ,  B01D15/363 ,  B01D15/3804 ,  B01J20/28033 ,  B01J20/286 ,  B01J20/3219 ,  B01J20/3253 ,  B01J20/3285 ,  B01J41/20 ,  C07K1/16 ,  C07K1/18 ,  C07K1/36 ,  C07K16/00 ,  C07K16/065 ,  Y10T29/49826

摘要： The present invention relates to a chromatography ligand defined by the following formula R1—R2—N(R3)—R4—R5 wherein R1 is a substituted or non-substituted phenyl group; R2 is a hydrocarbon chain comprising 0-4 carbon atoms; R3 is a hydrocarbon chain comprising 1-3 carbon atoms; R4 is a hydrocarbon chain comprising 1-5 carbon atoms; and R5 is OH or H. The invention also comprises a separation matrix, comprising the described ligands coupled to a porous support, such as particles or a membrane. The ligand and matrix according to the invention is useful for purification of biomolecules or organic compounds, such as proteins, polypeptides, DNA etc. An advantageous use according to the invention is the purification of antibodies.

摘要翻译： 本发明涉及由下式R1-R2-N（R3）-R4-R5定义的色谱配体，其中R1是取代或未取代的苯基; R2是包含0-4个碳原子的烃链; R3是包含1-3个碳原子的烃链; R4是包含1-5个碳原子的烃链; 并且R 5是OH或H.本发明还包括分离基质，其包含与多孔载体如颗粒或膜耦合的所述配体。 根据本发明的配体和基质可用于纯化生物分子或有机化合物，例如蛋白质，多肽，DNA等。根据本发明的有利用途是抗体的纯化。